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Practical 2
Labs 6-9
15
Microbiology
Undergraduate 4
12/04/2016

Additional Microbiology Flashcards

 


 

Cards

Term
Glucose/Lactose
Definition
Ferments glucose to produce an acid
Turns from red to yellow, and produces a gas bubble
Liquid test
Term
MR (Methyl Red)
Definition
Uses the fermented glucose to to produce organic acids
Turns red for positive and colorless for negative
Liquid test
Term
VP (Voges-proskauer)
Definition
Uses fermented glucose to produce neutral end-products
Turns bright pink if positive
Liquid test
Term
Indole
Definition
Tests for the breakdown of tryptophan to indole
Turns from red to yellow in presents of indole
Liquid test
Term
Mannitol Salts Agar (MSA)
Definition
Utilizes the mannitol as a source of carbon source
Positive turns yellow, negative stays red
Term
Blood Agar Plate (BAP)
Definition
Can destroy the red blood cells in the media
Beta-hemolysis--> colorless
Alpha-hemolysis--> greenish discoloration
Gamma-hemolysis--> no effect of red
Term
Oxidase
Definition
Presence of enzyme involved in respiration where oxygen is the terminal transporter
Term
Catalase
Definition
Enzyme that degrades hydrogen peroxide into pxygen and water
Bubbles form when bacteria added
Term
Motility Stab
Definition
The bacteria can move through the media
Term
Citrate Slant
Definition
Bacteria can use citrate as the carbon source and that also requires it use ammonium salts as a nitrogen source
Nitrogen salts releases ammonius black into media raising pH
Turns from dark green to persian blue
Term
Urease Slant
Definition
The bacteria can break down the urea into carbon dioxide and ammonia
Raise in pH causes media to go from yellow to bright pink
Term
Kligler Iron Slant: Aerobic
Definition
Red--> yellow : lactose fermentation and a change in pH from basic to acidic
Surface of tube
Term
Kligler Iron Slant: Anaerobic
Definition
Red-->yellow : glucose fermentation, pH drops from basic to acidic
Butt of tube
Term
Kligler Iron Slant: H2S
Definition
Reduces thiosulfate to H2S and causes a black parcipitate
Term
Kligler Iron Slant: Gas
Definition
Glucose and/or lactose fermentation
Bubbles visible in the media
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