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PMG exam 1 lecture 1
PMG exam 1
18
Science
Graduate
01/31/2009

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Term
1 linear chromosome and 1 circular
Definition
A.tumafaciens
Term
2 circular chromosomes
Definition
V.cholerae
Term
3 circular chromosomes
Definition
P.denitrificans
Term
Microbial Pan-Genome
Definition
all share core DNA but are differientiated by the added plasmids,pathogenicity islands, etc.
Term
average bacteria size
average chromosome size
Definition
1 um
1mm
Term
oriC consists of
Definition
AT-rich, 10-mers, DnaA box
Term
DnaA
Definition
binds DNA first to its boxes (multiple), DNA is wrapped around it to create a bubble at the AT region
Term
DnaC
Definition
binds bubble and enlarges it, loader protein (loads DnaB) converts ATP
Term
DnaB
Definition
helicase, keeps bubble open
Term
ssb
Definition
many bind and stabelizes
Term
E.coli termination protein
Definition
Tus, termination utilization substance
Term
B.subtilis termination protein
Definition
RTP, replication terminator protein
Term
termination sites
Definition
terA and ter B (face opposite directions
Term
recombination causes ---- which is resolved by------
Definition
dimers and 1 big chromo, each has 1 dif site, XerC and D bind each other and a dif site to resolve
Term
XerCD regulation
Definition
XerCD can only bind if FtsK is bound at the septum
Term
enzyme that resolves interwined chromosomes
Definition
Topisomerase IV
Term
condensins
Definition
bind and pull chromo's apart so Top IV can resolve
Term
Kleisins
Definition
bind to condensins and stabilize
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