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Plant Phys II
photosynthesis
76
Biology
Undergraduate 3
04/15/2007

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Term
what cell structure is essential to photosynthesis?
Definition
membranes
Term
what stage of photosynthesis reduces CO2 to CH2O?
Definition
Calvin cycle
Term
what stage of photosynth. oxidizes H2O?
Definition
Light rxtns
Term
what kingdoms perform photosythesis?
Definition
Plantae
Protista
Bacteria
Term
what are the 3 main events of the light rxtns?
Definition
1. O2 evolved from H2O
2. ATP produced
3. NADPH produced
Term
what powers the Calvin Cycle?
Definition
ATP and NADPH from the light rxtns.
Term
what organelle are chloroplasts derived from?
Definition
proplastids
Term
explain the Emerson Enhancement Effect and what it suggested?
Definition
plants exposed to light >690 nm and <690 nm have a higher photsynthetic rate than the sum of either one on its own.
suggested presene of 2 photosystems
Term
what is the absorption peak of photosystem I? II?
Definition
I - 700 nm
II - 680 nm
Term
which photosysytem is involved in cyclic phosphorylation?
Definition
PSI
Term
when is the rate of photosynthesis the highest?
Definition
when both cyclic and non-cyclic photophosphorylation are occurring
Term
what happens when isolated chlorophyll is exposed to light?
Definition
energy level of electrons boosted
red light and heat given off as they fall back down (fluorescence)
Term
what happens to chlorophyll exposed to light in a chlorplast?
Definition
electrons boosted to higher energy levels and transferred to electron carriers in the membrane
Term
what is a granum?
Definition
a stack of thylakoids (stacks of membranes)
Term
what are thylakoids?
Definition
pigment-containing 'sacs'
Term
what is the stroma?
Definition
the area between thylakoids
Term
where do the light rxtns take place?
Definition
in membranes
Term
where does the calvin cycle take place?
Definition
in the stroma
Term
what is the difference between grana thylakoids and stroma thylakoids?
Definition
grana thylakoids make up granum
-stroma thylakoids connect granum
Term
what strong reductant is produced by the light rxtns?
Definition
NADPH
Term
what is the name for the regions of the grana thylakoids that are in contact with the stroma? with each other?
Definition
contact w/stroma - non-appressed regions
each other - appressed regions
Term
what area of the thylakoid is photosystem II found?
Definition
the appressed region
Term
what are the events that occur in photosystem II?
Definition
1.light causes chlorophyll in rxtn center P680 to lose an electron
2.electron passed to pheophytin
3.photooxidized, moves to QA acceptor on D2 protein
4.moves to plastoquinone
5.moves to QB acceptor on D1 protein
6.reduced to plastoquinol
Term
where is the QA binding region located?
Definition
the D2 reaction protein in photsystem II
Term
where is the QB binding site located?
Definition
the D1 reaction protein of photosystem II
Term
what is the overall reaction for PSII?
Definition
2H2O + 4 photons + 2PQ + 4H+ ==> O2 + 4H+ + 2PQH2
Term
where do the 4 input hydrogen ions come from in the PSII reaction?
Definition
from the stroma
Term
where do the 4 output hydrogen ions come from in the PSII reaction?
Definition
from H2O
Term
what is reduced in the PSII reaction?
Definition
plastoquinone
Term
what structure transfers electrons from PSII to PSI?
Definition
cytochrome B6 - cytochrome F complex
Term
what 2 movements does the cytB6-cytF complex facilitate?
Definition
1.movement of electrons from PSII to PSI
2.movement of H+ from the stroma to the lumen
Term
where is PSII active?
Definition
appressed regions of grana thylakoids
Term
what are the 3 general structures at work in PSII?
Definition
1. intrinsic proteins
2. extrinsic proteins
3. Chlorophyll-Protein Complexes
Term
what parts of PSII are coded by chloroplast DNA? by nuclear DNA?
Definition
Chloro DNA - intrinsic proteins
Nuclear DNA - extrinsic proteins
Term
what are the main intrisic proteins at work in PSII? how many total?
Definition
D1 and D2
-6 total
Term
how many extrisic proteins at work in PSII?
Definition
3
Term
what are the 3 main Chlorophyl-Protein Complexes at work in PSII? what type and how many chlorophyll molecules a piece?
Definition
LHCII, CP47 and CP43
antennae chlorophyll
20-25 chlorophyll molecules a piece
Term
what parts of the cytochrome complex coded with chloroplast DNA? nuclear?
Definition
chloroplast - CytB6, CytF
nuclear - Fe-S protein
Term
where are CytB6-CytF complexes located?
Definition
equally in the grana and stroma thylakoids
Term
what are the 4 main parts of the cytochrome complex?
Definition
Cytochrome B6
Cytochrome F
Fe-S Protein
Compnent IV
Term
what is the electron path thru cytochrome complex?
Definition
1.platoquinol
2.Fe-S
3.CytF
4.Plastocyanin
Term
what are the main oxidation-reduction reactions of the light reactions?
Definition
PSII - H2O oxidized, plastoquinone reduced to plastoquinol
Cyt.Complex - plastoquinol oxidized/platocyanin reduced
PSI - plastocyain oxidized/ferredoxin reduced
Stroma - ferredoxin oxidized/NADP reduced to NADPH
Term
what ion carries the electron in plastocyanin?
Definition
Copper
Term
what ion carries the electron in PSI?
Definition
Iron
Term
what is the electon path in PSI?
Definition
1. plastocyanin
2. A
3. A1
4. Fe-S
5. Ferredoxin
6. NADPH
Term
what is the initial molecule oxidized in the light rxtns? final molecule reduced?
Definition
initial - H2O
final - NADPH
Term
where on the thylakoid is PSI active?
Definition
in the non-appressed regions of the grana and stroma thylakoid
Term
where on the thylakoid is PSII active?
Definition
on the appressed region of the grana thylakoid
Term
where on the thylakoid is ATPase active?
Definition
on the non-appressed regions of the grana and stroma thylakoid
Term
where on the thylakoid is the cytochrome complex active?
Definition
on the entire thylakoid (appressed and non-appressed regions of grana and stroma thylakoid)
Term
what is the equation for the cytochrome complex reaction?
Definition
2PQH2 + 4PC ==> 2PQ + 4PC + 4H+
Term
what ion involved in reduction of plastocyanin?
Definition
Cu2+
Term
what is the reaction for PSI?
Definition
light + 4PC + 4Fd ==>4PC + 4Fd
Term
what enzyme in the stroma catalyses the final OR reaction of the light reactions?
Definition
NADP reductase
Term
what 4 ions involved in PSII?
Definition
manganese
calcium
iron
chloride
Term
what replaces magnesium in pheophytin?
Definition
2H+
Term
what is the chlorophyll makeup of the LHCII?
Definition
a and b in 1:2 ratio (250 total molecules?)
Term
which LHC is more abundant in low light?
Definition
LHCII
Term
what is the chlorophyll makeup of the LHCI?
Definition
a and b in 1:4 ratio (100 total molecules)
Term
what does ATP synthase consist of?
Definition
9 polypeptides
Term
what is the head region of ATP synthase called? the stalk?
Definition
head - CF1
stalk - CF0
Term
what is the minimal #of photons needed to reduce 1 CO2 to 1 simpe sugar?
Definition
12 - 8 from non-cyclic, 4 from cyclic
Term
what is the output of non-cyclic photophosphorylation w/4photons?
Definition
8 H+ and 2NADPH
Term
what is the output of cyclic photophosphorylation alone with 4 photons?
Definition
4 H+
Term
what is the minimal ATP and NADPH needed to produce a simple carb?
Definition
3 ATP
2NADPH
Term
where do the 8 H+ ions from non-cyclic photophosphorylation come from?
Definition
4 from H2O (PSII)
4 from plastoquinol (originally from stroma)
Term
what is the PCR cycle?
Definition
Photosynthetic Carbon Reduction (Calvin Cycle)
Term
what are the compounds involved in the Calvin cycle?
Definition
1. Ribulose bisphosphate
2. Phosphoglyceric acid (PGA)
3. Phosphoglyceraldehyde (PGAL)
4. Ribulose 6-phosphate
Term
what is the precursor to sucrose/starch in the calvin cycle?
Definition
Fructose 6-phosphate
Term
what is the chain of reactions of the PCR cycle?
Definition
6RBP(5C) + 6CO2(1C) ==> 12PGA(3C) ==> 12 PGAL(3C) ==> 1 F6P(6C) + 10R6P(5C)
Term
what is known as the 'herbicide binding protein'? what site binds with herbicides?
Definition
D1 protein
QB site
Term
what crops are naturally resistant to herbicides that normally bind to the QB site? how are they resistant?
Definition
corn and cotton
sequester the herbicides in glands
Term
where step in the PCR cycle does Rbisco catalyse?
Definition
5RBP + 6CO2 ==> 12PGA
Term
how much ATP and NADPH needed to reduce 6CO2 to 1 glucose molecule?
Definition
18 ATP, 12 NADPH
Term
what steps in the PCR cycle are ATP and NADPH needed?
Definition
ATP - PGA to PGAL AND R6P to RBP
NADPH - PGA to PGAL
Term
what 4 specific events in photosynthesis require light?
Definition
1.ATP and NADPH production
2. Photolysis of H2O at OEC
3. activation of 5 enzymes in PCR including Rubisco
4. Coupling factor (ATPase) activation
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