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Plant Anatomy: Test 1
University of Guelph BOT*3410
372
Plant Sciences
Undergraduate 3
10/11/2016

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Term
Abaxial
Definition
Facing away from the stem; the lower side of a dicot leaf.
Term
Accommodative growth
Definition
A type of growth in epidermal cells. Produces pavement cells. Growth in response to neighboring cells. Can have characteristics similar to diffuse or tip growth.
Term
Acidic dyes
Definition
Stain by infiltration, and wash out easily.
Term
Adaxial
Definition
Adjacent to the stem axis; the upper side of a dicot leaf.
Term
Albuminous cells
Definition

"Nourish"

A cell type of phloem tissue, found only in gymnosperms. A sieve element. Generally elongated in shape. Has primary cell wall. Living at maturity, associated with sieve cells, but generally not clonal with sieve cells. Rich in cytoplasm, and nourish adjacent cells. Has numerous plasmodesmata connections with sieve cells. Plays a role in delivery of substances to and from sieve cells, including informational molecules and ATP.

Term
Algae
Definition
Nonvascular plants. Can move around like animals, but contain chloroplasts; defies classification. Some secrete a cell wall, allowing them to stick together in a colony of up to thousands of individuals. When you leave water exposed, algae are some of the first organisms to appear.
Term
Amphicribal
Definition
A rare type of vascular bundle. Phloem entirely surrounds the xylem. Found in the rhizomes of Pteridium aquilinum and other basal plants.
Term
Amphivasal
Definition
A rare type of vascular bundle. Xylem entirely surrounds the phloem. Found in the rhizomes of Acorus sp., sweet flag.
Term
Amyloplast
Definition
A plastid containing starch.
Term
Anatomy
Definition

"To dissect"

Aspects of an organism that you cannot see; inner structures. Often associated with human anatomy.

Term
Angular collenchyma
Definition
Collenchyma cells with thickenings in corners at right angles, with no intercellular spaces.
Term
Aniline blue
Definition
A botanical stain used to cellulose or callose. Callose stains blue under white or UV light.
Term
Anisocytic
Definition
A stomatal pattern. Stomata are surrounded by three subsidiary cells, all of different sizes.
Term
Anomocytic
Definition
A stomatal pattern. Epidermal cells around the guard cells all look similar. There no well-defined pattern.
Term
Anticlinal division
Definition
Divisions that occur parallel to the radial plane of the plant tissue. Cell wall forms perpendicular to the epidermis. Increases the number of cells in pre-existing layers.
Term
Antony van Leeuwenhoek
Definition
In 1674, described green charophyte algae Spirogyra as "green streaks, spirally wound serpent-wise", and Vorticella ciliates as "little animals fashioned like a bell". Called his microscope an "aalkijeker" meaning "eel viewer". He was not a scientist; he merely had a hobby of grinding high-quality lenses and using them to view microorganisms. Wrote many descriptions and letters.
Term
Apical meristems
Definition
Meristems found at the apices of the shoot and root. Generates primary meristems. The shoot and root apical meristems are connected by ground tissue in higher plants.
Term
Apoplastic
Definition

"Outside plasma"

Movement of water and solutes outside of the plasma membrane and protoplasts, in cell walls.

Term
Astrosclereid
Definition
A star-shaped sclereid with branches or arms radiating from a central body.
Term
Atrichoblast
Definition
A cell which does not give rise to a root hair. Rows alternate between trichoblasts and atrichoblasts.
Term
Autofluorescence
Definition
The generation of fluorescence due to endogenous metabolites and fluorescent compounds present in specimens. In plant cells it derives principally from chlorophyll (red fluorescence) and lignin (green fluorescecne). Emission is greatest when live cells are examined with blue or UV excitation wavelengths. Many cellular components and matabolites in plants have autofluorescence, including the inner walls of guard cells, lignified secondary cell walls, suberin, and the cuticle.
Term
Ballooning effect
Definition
When you are trying to visualize something smaller than the limit of resolution, a halo of fluorescence forms that is larger than the actual object.
Term
Bandpass filter
Definition
A coloured filter. Transmits light only within a defined spectral band ranging from less than one to many nanometers wide. May be used to protect the eyes from certain wavelengths.
Term
Barrier filter
Definition
Longpass and shrotpass filters. Transmit only above or below certain "cut-on" or "cut-off" wavelengths, and continues to transmit a wide energy band.
Term
Basic dyes
Definition
Stain by chemical absorption. Higher pH leads to more intense staining. Adheres to acidic components. Includes TBO.
Term
Berberine hemisulphate (FeCL3)
Definition
A botanical stain used to stain suberinized cell walls. Secondary cell walls, suberin, and the cuticle stain blue.
Term
Bicollateral
Definition
A type of vascular bundle. There is primary phloem on both sides of the xylem; external phloem and internal phloem.
Term
Black light
Definition
Non-visible wavelengths of light hit an object, and reflect back in a visible wavelength, making it seem that the object is emitting light. Can give things an odd colour.
Term
Blurriness
Definition
Occurs when objects are out of focus. Gives the impression of depth.
Term
Bordered pit
Definition
A pit where secondary cell wall overarches the membrane, formign appearance of a border.
Term
Botanical microtechnique
Definition

1. Preparation of tissues.

2. Use of microscope and related equipment for critical study and interpretation of material.

3. Clear and concise description.

4. Recording and illustrating.

Term
Brachysclereid
Definition

Stone cell

A short, and roughly isodiametric sclereid.

Term
Bright field microscope
Definition
The specimen is illuminated from below. This is the most common form of microscopy.
Term
Bud primordia
Definition

Axillary bud

Found in leaf axils. Resemble the SAM, but are dormant due to SAM-induced apical dominance and auxin and strigolactone signalling. If the SAM is removed, the bud primordia are released from dormancy.

Term
Bulge formation
Definition
The second step of root hair devleopment. A bulge forms on the trichoblast.
Term
Bulliform cells
Definition

Motor cells

Thin-walled epidermal cells of grass that are larger and more inflated than neighboring cells. Create bands that run parallel to the long axis of the leaf. During drought, they allow grass leaves to close as the two sides of the blade fold up towards each other. Movement of the leaf blade is regulated by loss and uptake of water in bulliform cells. When rain comes, the leaves unfold and turn green again.

Term
Calcofluor
Definition
A botanical stain used to stain cellulose cell walls.
Term
Callose
Definition
A component of cell walls. A carbohydrate that hydrolyzes to glucose residues. A linear glucan deposited between plasma membrane and existing cellulosic cell walls. Deposited very rapidly in response to wounding, sealing plasmodesmata and isolating cells from the phloem. Found on sieve plates, in phloem, in pollen tubes, and sometimes in parenchyma cells. In winter phloem fills with callose plugs, which dissolve in the springtime. May be stained with aniline blue, which stains it blue under white or UV light.
Term
Callus
Definition
Undifferentiated plant cells that form from de-differentiation in medium.
Term
Cambium
Definition
Meristems that carry out periclinal divisions so that cells are arranged in radial files, and contribute in two directions. Located between the phloem and xylem in primary tissue. In older cells, especially at the base of the stem, the cambium can expand.
Term
Cashew
Definition
In the same family as poison ivy. Poisonous, but the poison is removed with processing.
Term
Casparian strip
Definition

Casparian band

A thickened layer in the endodermis surrounding the cortex and endodermal cells. Breaks the apoplastic flow of water towards the xylem, stopping water and photosynthates from leaking out of the vascular system.

Term
Cavitation
Definition
Cavities in the vessel column, resulting in its breaking.
Term
Cell biology
Definition
Deals with the structure and functioning at the cellular and sub-cellular level.
Term
Cell plate assembly matrix (CPAM)
Definition
Microtubules of the phragmoplast terminate in the CPAM, and direct Golgi-derived vesicles to teh cell plate during telophase.
Term
Cell specification
Definition
The first step of root hair development. Cells differentiate into trichoblasts and atrichoblasts.
Term
Cell wall (CW)
Definition
A defining characteristic of plant cells. Surrounds and anchors the protoplast. Has complexity and architecture which dictates and reflects on cell function. Found in plant cells, bacteria cells, fungi cells, and in some animal cells (choral polyps). It is an extracellular matrix secreted by the cell. Components include cellulose, hemicellulose, pectin, callose, lignin, cutin, and suberin.
Term
Cellufluor
Definition

Calcofluor white M2R

A dye which makes cellulosic components such as primary cell walls fluoresce blue-white under UV light. Enhances the property of autofluorescene.

Term
Cellulose
Definition

(C6H10O5)n

The primary component of cell walls. A polysaccharide of beta (1→4) linked glucose monomers. A linear chain whcih forms microfibrils with spaces between them ranging from 5 - 30 nm. The most common compounds in plants. May be stained with fast green, aniline blue, or calcofluor.

Term
Centrioles
Definition
Not found in higher plants, but found in animal cells.
Term
Charles Darwin
Definition
Believed that plants could think, and that their brains were located in the roots.
Term
Chernobyl
Definition
After the nuclear disaster, people evacuated the area to avoid radiation. Plants remained behind and were able to survive.
Term
Chlamydomonas
Definition
An algae which resembles chloroplasts, and may share a common ancestor with them.
Term
Chlorenchyma
Definition
Parenchyma cells with chloroplasts. Green in colour.
Term
Chloroplast
Definition
The "green slave" of a plant. A green plastid that can move around inside the cell in response to light. Pleomorphic, and can have stromules. Have characteristic grana. Not all plant cells have chloroplasts. They can have binary fission, but they never fuse together. All chloroplasts are slightly different.
Term
Chromoplast
Definition
A plastid with vibrant colour. Found in flowers.
Term
Chromosomes
Definition
Found in both plant and animal cells.
Term
Cilia
Definition
Found in some reproductive plant cells, as well as in complex animal cells.
Term
Circumnutation
Definition
As seedlings emerge, growth in the stem is not equal, and causes circling, or waving motion of the seedling.
Term
Clonal
Definition
Originating from the same parent cell. Companion cells are clonal with sieve-tube cells, but albuminous cells are not clonal with sieve cells.
Term
Cohesion-tension theory
Definition
Drives movement in xylem. Water in transported along a water potential gradient, assisted by strong cohesion of water molecules due to hydrogen bonding, and tension in tracheary elements.
Term
Collateral
Definition
The most common type of vascular bundle. The eustele has xylem towards the inner side, and phloem towards the outer side. Typical of dicots.
Term
Collenchyma
Definition
A cell type of ground tissue. Located at the periphery of some aerial organs; in young elongating stems, and in ribs along veins of some leaves. Provide mechanical support to elongating organs. Elongated shape, with unevenly thickened cell walls, lacking lignin but rich in pectin. Living when functional. Types include angular, lacunar, and lamellar.
Term
Collet
Definition

Collar

The junction where the shoot meets the root.

Term
Collet hair
Definition

Collar hair

A root hair that grows on the collet. Grow synchronously with each other. There is no differentiation between hair files. The collet cells of a seedling expand, bringing their nuclei with them, anchoring the plant in the soil.

Term
Colleter
Definition
A multicellular appendage that produces sticky secretions. Common on buds and young leaves. Sundews have them.
Term
Companion cell
Definition
A cell type of phloem tissue, found only in angiosperms. A sieve element. Variable shape, generally elongated. Has primary cell wall. Living at maturity. Closely associated and clonal with sieve-tube elements, connected with numerous branched pore-plasmodesmata. Metabolically active, nucleate parenchyma cells. Movement of organic material relies on physiological interaction between sieve-tube elements and companion cells. Plays a role in delivery of substances to the sieve-tube element, including informatinal molecules and ATP. Loads sugars into the sieve tube element in source tissues, and unloads sugars in sink tissues. Some have transfer cells.
Term
Confocal laser scanning microscope (CLSM)
Definition
A microscope that uses lasers, and allows for optical sectioning. With electronic additions, it allows digial image acquisition. A pinhole is conjugate to the focal point of the pens:a confocal pinhole. The focal point of the objective lens forms an image where the pinhole is, and these two points are known as conjugate points. Removes out of focus light. Does not provide a complete 3D image at once, but can provide a stack of images that can then be collapsed to reconstruct the complete scanned object, built into a 3D projection. It can read a book without opening it, scanning layer by layer!
Term
Contrast
Definition
The ability to see something which is different from its background.
Term
Corpus
Definition
An inner layer of the SAM. Divides periclinally, adding new layers to the corpus.
Term
Cortex
Definition
The layer of cells between the cambium and epidermis. Usually parenchyma cells containing chloroplasts. Peripheral zones can be collenchyma, in strands or layers Inner zones may have a starch sheath.
Term
Counterstain
Definition
A stain that enhances the contrast of an image by colouring the background rather than the specimen. Makes structures more visible when not adequately highlighted by the primary stain.
Term
Crooked Forest
Definition
A forest in Poland where the trees are all bent in the same direction. Someone might have pulled the trees down into that shape. Sows how plant cell walls have both plastic and elastic properties; the trees can bend, and then harden into the shape they are bent into. Or it might be proof of aliens!
Term
Cryotome
Definition

"Freezing-cutting"

An instrument which cuts very thin slices of materials in a liquid nitrogen environment. Produces a snapshot of what is happening, however ice crystals can damage cell membranes. Sugar improves plant structures.

Term
Crystal
Definition
Crystals of substances can form inside plant cells, especially in large vacuoles. May be salt crystals.
Term
Crystal violet
Definition
A botanical stain used to stain lignified cell walls or cutinized cell walls.
Term
Cuticle
Definition
A thin, waxy layer of non-cellular material, coating the exterior surface of the epidermis. Covers the outer surface of the primary shoot. Appears as a transparent, colourless, or pale grey layer. Separated from the epidermal cells with a pectinaceous layer. Composed of cutin and epicuticular waxes. An essential feature of aerial portions of plants, limiting water loss, protecting the plant from environmental stresses including insects, and reflecting harmful radiation. May be stained with Sudan III/IV.
Term
Cutin
Definition
A component of the cell wall. Insoluble, hydrophobic lipid polymer. Synthsized by hydroxylation and esterification of fatty acids. Forms a matrix in which waxes are embedded to prevent loss of water. A component of the cuticle. May be stained with erythrosine or crystal violet.
Term
Cutting
Definition
A plant cutting will produce adventitious roots which grow into the soil.
Term
Cystolith
Definition
A knobby, stalked concretion of minerals, usually calcium carbonate, that occurs in lithocysts. Often is attached to the edge of the cell by a cellulosic peg, and projects into the lumen of the cell. May completely fill the cell.
Term
Cytoplasm
Definition
May be stained with eosin-Y, erythrosine-B, or fluorescein isothiocyanate.
Term
Cytoplasmic streaming
Definition
Cyclic flow of the cytosol while the cell undergoes physical processes. Carries organelles and vesicles around the cell. Helps in dispersal of nutrients. Prevents local build-up of harmful cellular by-products. Responsive to light, heat, pH, and ionic gradients. Involves the cytoskeleton. Leads to exudation of secretions. Can be readily observed in many epidermal cells.
Term
DAPI
Definition

4,6-diamidino-2-phenylindole

A botanical stain used to stain nuclei.

Term
De-differentiation
Definition
Plant cells are placed on medium, and proliferate into a callus.
Term
De-etiolation
Definition
Caused by light. Cotyledons open, expand, and turn green. Leaves develop, and hypocotyl growth rate is reduced. Etioplasts turn into chloroplasts.
Term
Defenestration
Definition
A fancy word for throwing something or someone out of a window!
Term
Depth of field
Definition
The distance between the nearest and farthest object that appears sharp in the image.
Term
Desert saltbush
Definition

Atriplex sp.

Halophytes which secrete salt from their salt glands.

Term
Diacytic
Definition
A stomatal pattern. There are two subsidiary cells at a right angle to the pore axis.
Term
Dichroic beamsplitter
Definition
A mirror that reflects light shorter than a certain wavelength, and passes light longer than that wavelength.
Term
Dicot
Definition

Eudicot

A type of flowering plant that has branchign leaf veins, two cotyledons, and vascualr bundles arranged in a ring around the pith. Walls near the stoma pore of guard cells are thicker.

Term
Dictysome
Definition

Golgi body

Disappear from the sieve element protoplast.

Term
Differential interference contrast (DIC)
Definition
A form of microscopy which uses interference of light to produce contrast.
Term
Differentiation
Definition
A cell develops structures different from other cells. After full differentiation, plant cells cannot divide.
Term
Diffuse growth
Definition
A type of growth in epidermal cells. Equal growth on all sides of the cell, expanding into a sphere. Can also include elongation of cells.
Term
Division
Definition
A cell divides and becomes two. Includes periclinal and anticlinal divisions.
Term
Dye
Definition
Increases contrast, and can label specific components. Unlike a stain it is permanent. May be used to create a small stain. Includes basic and acidic dyes.
Term
Elasticity
Definition
A property of the primary cell wall. Returns to its original size after deformation. Necessary for the plant to grow the right way. Decreases as the cell wall thickens.
Term
Embolism
Definition
Blockage of the conduit with air and vapour or ice, making a vessel dysfunctional. May be caused by sudden change in temperature leading to change of state of the water within the vessel: freezing or evaporation.
Term
Endarch
Definition
Protoxylem cells are found towards the centre of the tissue in comparison to metaxylem. There is centrifugal maturation; adding from the inner side. Smaller cells are seen in the centre. Typical of stems.
Term
Endodermis
Definition

"Inner skin"

A layer of cells which surrounds the stele in the root.

Term
Endoplasmic reticulum
Definition
Found in both plant and animal cells.
Term
Endosomes
Definition
May be stained with FM 4-65.
Term
Eosin-Y
Definition
A botanical stain used to stain cytoplasm.
Term
Epicuticular wax
Definition
Very long chain fatty acid derivatives. A component of the cuticle. Synthesized from fatty caids, and secreted by epidermal cells. Appears as a glaucous layer in some plants. Can form as cracked wax crystals, wax chimneys around stomata, wax tubules, or wax platelets.
Term
Epidermal cells
Definition
Cells of the epidermis. Produce and secrete epicuticular waxes to form the cuticle. Undergo division and differentiation. Exhibit diffuse, tip, and accommodative growth. Large cells in which cytoplasmic streaming can readily be observed. Many lack chloroplasts.
Term
Epidermis
Definition

"Upon skin"

Dermal tissue system. A primary tissue. The outermost layer of cells on the primary plant body. Arises from the outermost cell layer of the apical meristem; the protoderm. Usually it is one cell layer thick, but sometimes multiseriate. Coated by the cuticle.

Term
Epifluorescence
Definition
A type of microscopy where the image comprises of rays from multiple points on the object. Some rays are in sharp focus, and others less so. A certain degree of blurriness is always associated.
Term
Epithem
Definition
Open, arenchymatous parenchyma which form the bulk of the glandular tissue of a hydathode.
Term
Ergastic
Definition
Non-living materials within or around the cell.
Term
Erythrosine
Definition
A botanical stain used to stain cutinized cell walls.
Term
Erythrosine-B
Definition
A botanical stain used to stain cytoplasm.
Term
Etioplast
Definition
A plastid. It is whitish in colour. Occurs when the plant is elongating, and is etiolated. Becomes a chloroplast when the seedling de-etiolates.
Term
Eustele
Definition
The earliest form of stele. The primary phloem and primary xylem arranged along the same radius. Has one area of phloem, and one area of xylem. May be collateral or bicollateral.
Term
Evans blue
Definition
A vital stain excluded by living cells.
Term
Exarch
Definition
Metaxylem cells are found towads the centre of the tissue in comparison to protoxylem. There is centripetal maturation; adding to the outer side. Larger cells are seen in the centre. Typical of roots and seed plants.
Term
Excitation maximum (EXM)
Definition
The amount of energy needed to excite a molecule.
Term
Extension growth
Definition

Growth

The third step of root hair development. Includes a stage of slow extension, followed by a stage of rapid extension.

Term
F-actin
Definition
In plants, most organell movement occurs along F-actin. Organelle motility appears erratic.
Term
Fast green
Definition
A botanical stain used to stain cellulose cell walls.
Term
Fibre
Definition
Sclerenchyma cells that are long and narrow with a thick secondary cell wall. Not always dead when functional. Located in the cortex of stems, often associated with xylem and phloem, and in the leaves of monocots. Function in support.
Term
Fibre (phloem)
Definition
A cell type of phloem tissue. Generally elongated. Has primary and secondary cell wall; lignified and pitted. Dead when functional. Functions in mechanical support.
Term
Fibre (xylem)
Definition
A cell type of xylem tissue. Elongated and tapering in shape. Has thick primary and secondary cell wall, lignified, and with bordered pits. Dead when functional. Functions in mechanical support. The main woody cells of making up xylem are fibres.
Term
Fixed
Definition
Dead tissues which do not respond. Usually the tissue is soaked in chemicals to kill the cells.
Term
Flagella
Definition
Found in some reproductive plant cells, as well as in complex animal cells.
Term
Fluorescein isothiocyanate (FITC)
Definition
A fluorescent probe used to stain cytoplasm.
Term
Fluorescence
Definition
Emission of light after absorption of light or other forms of radiation. Allows you to see different things in microscopy. A short wavelength of light is absorbed, and a quickly re-emitted. Energy is lost, and a lower wavelength is emitted.
Term
Fluorescence microscopy
Definition
Relies on excitation of fluorescent molecules with a specific wavelength region to produce an image generated by the secondary fluorescence emission at longer wavelengths.
Term
Fluorescent probe (FB)
Definition
Produces high signal to noise ratio. Includes organic dyes, biological fluorophores, and quantum dots. New fluorescent probes are produced as needed. They remain stable after induction, and maintain clear imaging parameters. Can be incorporated into existing infrastructure. Includes GFP.
Term
FM 4-64
Definition

N-(3-triethylammoniumpropyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide

A membrane-selective permeant fluorescent probe dye used to stain plamsa membrane, endosomes, and tonoplasts.

Term
Gap junction
Definition
Small junctions between animal cells. Analogous to plasmodesmata.
Term
Genetically modified organisms (GMO)
Definition
Can be used in research to learn about plants. Structures in living cells can be seen in fluorescent protein transgenic organisms.
Term
Glandular trichome
Definition
Trichomes with unicellular or multicellular heads. The heads may be filled with secondary metabolites harmful to insects. Found on cannabis and tobacco leaves. In tobacco, the heads are filled with nicotine.
Term
Glaucous
Definition
A waxy cuticle secretion which is shiny from certain angles. Appears as a blue-purple covering on the skin of fruit such as plums and grapes.
Term
Glyoxysomes
Definition
Found in plant cells, but not animal cells.
Term
Golgi complex
Definition
Found in both plant and animal cells. In plant cells there are many Golgi bodies; they are needed to make material to create the cell wall. In animal cells there is normally just one Golgi body.
Term
Grana
Definition
Stacks of thylakoids found in chloroplasts. The first part of photosynthesis occurs here.
Term
Green fluorescent protein (GFP)
Definition
A fluorescent probe originally isolated from a jellyfish; this work won a Nobel Prize. The gene can be coded and inserted into other organisms.
Term
Ground meristem
Definition
A primary meristem. Produces ground tissues.
Term
Ground tissue
Definition
A primary tissue. Includes parenchyma, collenchyma, and sclerenchyma. Produced by the ground meristem.
Term
Guard cell
Definition
Part of the stomatal complex. Swelling is regulated by turgor pressure generated by K+ ions, for opening and closing of stomata. Symplastically isolated, with thicker inner cell wall. Fluoresce yellow-orange under UV or blue light. May be at the same level as epidermal cells, or sunken in stomatal crypts. Has chloroplasts.
Term
Growth
Definition
Irreversible increase in cell size.
Term
Guttation
Definition
The process of secreting excess water. Normal in some plants. Cells of the epithem absorb mineral nutrients from xylem sap, and transfer them to the leaf tissues. Excess water accumulates in intercellular spaces, and is forced out through the hydathode water pores. Often occurs in the morning, especially in indoor plants. Usually occurs in plants which have leavs with tapers. Microbes can enter the plant from the water droplets. Common in over-watered houseplants.
Term
Half-plasmodesmata
Definition
Plasmodesmata which form on the epidermal layer, where there are no neighboring cells to connect with. The channel is half-formed, and is plugged with callose.
Term
Halophyte
Definition
A plant that grows in a saline habitat. Includes the desert saltbush. Has salt glands.
Term
Hechtian strands
Definition
A portion of the plasma membrane which stretches between protoplast and cell wall when a cell is plasmolyzed.
Term
Hemicllulose
Definition
A component of cell walls. Composed of various sugar monomers. A heterogenous group of non-crystalline glycans including xyloglycans, glucuronoarabinoxylans, xylans, and glucomannans.
Term
Hydathode
Definition

"Water hole"

A structure which discharges water and dissolved substances to the leaf surface during guttatioin. The bulk of glandular tissue is epithem.

Term
Hypocotyl
Definition
The region below the cotyledon(s) and above the root. Primary walls are able to stretch considerably with no extra wall formation. Stretches to find light before de-etiolation.
Term
Hypodermis
Definition

"Below skin"

The layer of cells just below the epidermis. Thickenings in this layer may give off yellowish autofluorescence.

Term
Idioblast
Definition
A cell in the cortex or pith that differs markedly in form, size, or content from surrounding cells. May be a sclereid, or have crystals or silica grains.
Term
Image
Definition
Every point of light has a sharp focal point, and they all dissolve together to form an image.
Term
Imperforate
Definition
Has pits in the lateral cell wall. Tracheids have imperforate cell walls.
Term
Incident light
Definition
Light from above which the specimen absorbs or reflects.
Term
Indumentum
Definition
A coat, or covering of fine hair. Types include velvety, pubescent, silky, felted, tomentose, woolly, villous, pilose, ciliate, and fibreate.
Term
Insect galls
Definition
When an insect damages plant materials, the wounds fill with callose. This can prevent some of the cells from communicating with the rest of the plant, and the cells start to grow independently, and can form a gall. Many pathogens can cause this.
Term
Intercalary meristems
Definition
Meristems derived from apical meristems, and inserted between tissues that are no longer meristematic.
Term
Intercellular spaces
Definition
They can be large; there are thin cell walls, surrounding air spaces. Can be useful for a floating hydrophyte.
Term
Iodine-potassium iodide (I2KI)
Definition
A botanical stain used to stain starch grains. Stains starch grains purple or bluish-black.
Term
Iron hematoxylin
Definition
A botanical stain used to stain middle lamella.
Term
Jellyfish
Definition
Certain species can be fluorescent under blue or green light. GFP was originally isolated from a jellyfish.
Term
Jewel weed
Definition
Used to relieve pain from scorpion stings and poison ivy.
Term
Lacunar collenchyma
Definition
Collenchyma cells with walls thickened in the corners of cells, with intercellular air spaces in the tissue.
Term
Lamellar collenchyma
Definition
Collenchyma cells with a thin layer along tangential walls. Inner and outer tangential walls are thickened.
Term
Lateral meristems
Definition
Meristems parallel within an axis. Includes the vascular and cork cambium. Meristematic cells are deposited at intervals within the roots, and can develop into lateral roots.
Term
Leaf primordia
Definition
Develop into leaves with proliferation of epidermal and ground tissue cells, as well as branching of the stem's procambium into each leaf.
Term
Light
Definition
Usually refers to visual light, but is actually a very wide spectrum of wavelengths, of which visual light is a small fraction. White light is a mixture of all visible colours. With visual light, the limit of resolution is 200 nm.
Term
Lignin
Definition
A component of cell walls. A phenolic polymer. A binding agent with compressive strength. Lignification is an irreversible process; a hydrophobic filler replaces the cell wall water. Found in cells with secondary cell wall thickenings, such as mature sclerenchyma fibres, and xylem tracheary elements, such as in wood. May be stained with safranin, crystal violet, TBO, or phloroglucinol. TBO stains it blue or green.
Term
Limit of resolution
Definition
Depends on the wavelength of light. If you decrease wavelength, you increase the limit of resolution. The limit of resolution is generally about half the wavelength of light. If you try to look at something smaller than the limit of resolution, there is ballooning effect.
Term
Lithocyst
Definition

"Stone cell"

A cell which contains a cystolith. The epidermal cells of certain plants. Sequesters calcium carbonate. Often found in plants growing in alkaline soils high in calcium salts. Causes the grittiness of ficus leaves.

Term
Lotus
Definition
A floating hydrophyte which sinks below the water at night, and rises up and opens on the surface of the day.
Term
Lysosomes
Definition
Found in plant cells as a type of vacuole, and are common in animal cells.
Term
Maceration
Definition
To tear apart. A way to prepare tissues. Used for succulent tissue; it is crushed or smeared into a thin layer.
Term
Macrofibrils
Definition
Coils of microfibrils. 0.5 μm in diameter, and 4 - 7 μm in length.
Term
Macrosclereid
Definition
An elongated, columnar or rod-shaped sclereid with uneven secondary wall thickenings.
Term
Magnification
Definition
Making an image appear larger. Achieved through use of a lens.
Term
Maple syrup
Definition
When tapping for maple syrup, a tap is drilled about an inch deep into a maple tree, into the xylem of the tree, not the phloem as is commonly thought. Sugary water is brought up from the roots in the spring through the xylem, for energy for growing leaves. Warm temperatures melt the fluid in the xylem, and sugar prevents damage from freeze-thaw fluctuations.
Term
Marram grass
Definition

Ammophila arenaria

A plant with bulliform cells.

Term
Mega mitochondria
Definition
Large mitochondria present when the body has no oxygen.
Term
mEosFP
Definition
Produces differential colouring to allow behaviour of similar organelles to be investigated. Probes for plants include plasma membrane, cytosol, cytoskeleton, nucleus, mitochondria, plastids, endoplasmic reticulum, Golgi bodies, vacuoles, peroxisomes, autophagosomes, and endosomes. Specific gene promoters of fluorescent proteins can be expressed in precisely defined locations, and anatomical details become fluorescently highlighted in stable transgenic plants.
Term
Meristem
Definition
Areas in the body of a plant where there is addition of new cells. Localized regions of actively dividing cells. Produce new cells and organs. Can be apical or lateral. Growing leaves have some meristematic activity. Meristems can form in tissue culture, due to a plant disease, or for wound healing.
Term
Meristematic cells
Definition
Small, nearly isodiametric cells. Cytoplasmically rich, with small vacuoles. Have thin walls, little storage material, and pro-plastids. The nucleus takes up most of the space inside the cell.
Term
Mesophyll cells
Definition
In an experiment, mesophyll cells from a non-woody plant were turned into woody tracheary cells by alternating hormone balance; this is transdifferentiation.
Term
Metachromatic dye
Definition
A dye which produces more than one colour, depending on the properties of the specimen. Includes TBO.
Term
Metaxylem
Definition
Primary xylem that develops after the protoxylem. Forms wider vessels and tracheids. Tracheids are generally longer than protoxylem tracheids.
Term
Microfibrils
Definition
Chains of cellulose. Coil around one another to form macrofibrils. Embedded in a cross-linked matrix of non-cellulosic molecules.
Term
Microtome
Definition
An instrument that cuts very thin slices of plant materials embedded in paraffin wax. The razor must be sharpened regularly.
Term
Middle lamella (ML)
Definition
The first part of the true cell wall layer to develop. Very thin, located between adjoining cells. Consists of hydrated pectinaceous substances. Optically isotropic; under a light microscope it is usually at the limits of resolution, and appears as a black line. May be stained with ruthenium red or iron hematoxylin.
Term
Mitochondria
Definition
Found in both plant and animal cells. Organelles that create and manage energy gradients. 0.1 - 1.0 μm in diameter. They are smaller when the organism has sugars, and larger and longer when the organism is in a state of low energy such as when it is hungry or sleeping; size and number are controlled by fusion and fission. Canf form mega mitochondria May be stained with rhodamine 1,2,3 or mitotracker.
Term
Mitotracker
Definition
A fluorescent probe used to stain mitochondria.
Term
Molecular cellular biology
Definition
Attempts to understand details at the molecular level.
Term
Monocot
Definition
A type of flowering plant that has parallel leaf veins, one cotyledon, and vascular bundles distributed randomly throughout the ground tissue. End walls of guard cells are thicker, with a relatively thinner middle region. End portions of guard cells are bulbous, giving them a dumbell shape. Stomata open when bulbous ends are turgid, and guard cells are connected to each other by a pore in the bulbous polar regions.
Term
Morphology
Definition
Aspects of an organism that you can see.
Term
Multiseriate
Definition
Consisting of multiple series, or layers of cells.
Term
Nacreous
Definition
Shiny, changing colour when you move it, like mother-of-pearl. Sieve elements appear nacreous in fresh sections.
Term
Nectary
Definition
A multicellular glandular structure that secretes sugary fluid. Includes floral and extrafloral nectaries.
Term
Neutral density filter
Definition
A grey filter. Lowers the amount of light. Uniformly attentuates the intensity of light over a broad spectral range.
Term
Noah's Ark
Definition
It is not mentioned in this story if Noah brought plants along with him. Plants do not need to be saved; they can survive almost anything.
Term
Node
Definition
An area where one or more leaves are attached to the stem. Leaf and bud primordia are located here.
Term
Nonvascular plants
Definition

Lower plants

Plants that lack a vascular system. "Lower plant" is inaccurate, because they co-exist with vascular plants, and have been evolving for as long. Includes algae and moss.

Term
Nucleus
Definition
Found in both plant and animal cells. May be stained with DAPI, propidium iodide, SYTO dyes, or TBO. TBO stains it blue-green.
Term
Numerical aperture (NA)
Definition

Defines the information-collecting capacity of any lens system. The upper limit of NA is set by the refractive index of the medium in which the optical system is operating.

NA = n sin u

n = the refractive index of the medium in which the optical system is operating (air, n = 1.0; water, n = 1.33)

u = the half-angle subtended by the aperture of the optical system

Term
Optical highligher
Definition
Fluorescent proteins that are photo-convertible, photo-inducible, or photo-switchable.
Term
Optical sectioning
Definition
Sectioning using light in a confocal laser scanning microscoe. Uses light rather than a razor.
Term
Optically isotropic
Definition

Transparent

A substance with the same optical properties along all axes.

Term
Osteosclereid
Definition
A bone-shaped sclereid with enlargements at the ends.
Term
P-protein
Definition

Slime plug

A proteinaceous substance formed by the sieve element protoplast. Forms tubular filaments that helps in plugging.

Term
Paracytic
Definition
A stomatal pattern. There are two subsidiary cells parallel to the pore axis.
Term
Paradermal section
Definition
A section which removes only the outermost cell layer from a plant organ.
Term
Paraffin wax
Definition
The water in plant cells is replaced with paraffin wax before being sectioned in a microtome; this takes a few days. The wax will melt and stick to the slide, and a series of alcohol washes removes the wax.
Term
Parenchyma
Definition
A cell type of ground tissue. Located in all plant organs: pith, xylem, and phloem. Performs functions including storage, secretion, conduction, and wound healing and regeneration. Totipotent; have living protoplast and can regenerate the entire plant in suitable conditions. Living when functional. Can assume various shapes depending on location; commonly polyhedral. Cells have uniformly thin primary cell walls, usually lacking lignin. Some deposit a secondary cell wall which may become lignified, such as parenchyma cells in the pith and cortex of old roots and stems of monocots, and in xylem. Cell wall may contain lignin, suberin, or cutin.
Term
Parenchyma (phloem)
Definition
A cell type of phloem tissue. Variable in shape, usually polyhedral. Has thin primary cell wall. Functions in storage and transfer.
Term
Parenchyma (xylem)
Definition
A cell type of xylem tissue. Variable in shape, usually polyhedral. Cell wall is often lignified. Can become a tylose. Functions in storage and sealing of tracheary elements.
Term
Pavement cells
Definition
Epidermal cells which form most of the surface of a leaf or stem.
Term
Pectin
Definition
A major component of cell walls. Forms a gel in which the cellulose-hemicellulose network is embedded. A diverse collection of polysaccharides secreted by the Golgi apparatus. Contains several structural proteins including glycoproteins, extensins, and arabinogalactan proteins. Rich in galacturonic acid. Found in primary cell walls of parenchyma, collenchyma, phloem sieve tube cells, and phloem companion cells. May be stained with TBO; it stains pink.
Term
Peltate trichome
Definition
A scale-like trichome. Helps the plant conserve water. Found on the leaves of olive trees.
Term
Olive
Definition

Olea europeae

A plant with peltate trichomes on its leaves.

Term
Perforation plate
Definition
An area on the ends of vessel members. May be simple, with one large opening, or perforated.
Term
Peiclinal division
Definition
Divisions that occur parallel to the tangential plane of the plant tissue. A cell wall forms parallel to the epidermis. Produces new series of cells, creating new layers of cells.
Term
Pericycle
Definition
A layer of the endodermis in roots.
Term
Peripheral growth zone (PGZ)
Definition
The cell plate assembly matrix directs Golgi-derived vesicles to the cell plate. Vesicle fusion creates tubule-vesicular membrane network which expands outwards and fuses with parent cell walls during telophase.
Term
Peripheral zone
Definition
A zone of the SAM where cells divide frequently.
Term
Peroxisomes
Definition
Common in plant and animal cells.
Term
Phloem
Definition
A tissue of the vascular system. Transports sugars generated by photosynthesis from source tissues to sink tissues. Movement is driven by pressure-flow hypothesis. Except for fibres, all cells have thickened primary cell wall and no secondary cell wall, and are alive at maturity. Cell types include sieve cell, albuminous cells, sieve-tube elements, companion cells, fibres, and parenchyma.
Term
Phloroglucinol
Definition
A botanical stain which contains HCl, and is useful as a positive indicator of lignin. Stains lignin red or yellowish-red. Secondary cell walls stain red.
Term
Photo-convertible
Definition
An optical highlighter. Fluorescent, but changes colour permanently when exposed to light of a particular wavelength.
Term
Photo-inducible
Definition

Photo-activable

An optical highlighter. Not fluorescent until it is exposed to light of a particular wavelength. Without the light, you cannot know if the protein is present.

Term
Photo-switchable
Definition
An optical highligher. Fluorescent, but changes colour when exposed to light of a particular wavelength, and changes back to its original colour when exposed to light of another particular wavelength.
Term
Photobleaching
Definition

Fading

The permanent loss of fluorescence by a molecule due to photon-induced chemical damage or covalent modification. Occurs when the speciment is exposed to too much light. To avoid, do imaging as quickly as possible, and let the sample recover from brief periods of light exposure.

Term
Phragmoplast
Definition

"Fragmented"

A scaffold for cell plate assembly that leads to the separation of daughter cells during telophase. Initials arise within clusters of polar microtubules and actin filaments during late anaphase.

Term
Pit
Definition
A cavity where primary cell wall is not covered by secondary cell wall. Gives a porous surface to sieve elements. Includes bordered pits.
Term
Pit membrane
Definition
The portion of the compouond middle lamella that traverses a pit. Permits intercellular transport of water and soluble materials.
Term
Pith
Definition

Medulla

The tissue in the centre of the stem, surrounded by the cambium. Parenchyma cells; ground tissue of the stem. The internodes may be crushed with prominent intercellular spaces.

Term
Pith meristem zone
Definition
A zone of the SAM where cells divide frequently.
Term
Plant anatomy
Definition

Phytotomy

Involves preparation of tissues, observing, and describing the internal structures of plant organs. Linked to survival of the plant. Deals with the internal structure of the plant body.

Term
Plant morphology
Definition
Involves observing and describing the external structures of a plant. Deals with whole plants, or intact organs. Linked to survival of the plant.
Term
Plasma membrane
Definition

Plasmalemma

Found in both plant and animal cells. May be stained with FM 4- 64.

Term
Plasmodesmata
Definition
Intercellular cytoplasmic channels that traverse cell walls. 30 - 50 nm thick. Spaces in the primary cell wall formed from site where strands of endoplasmic reticulum became trapped in the developing cell plate after mitosis. Allow for symplastic transport. May be distributed in primary cell walls uniformly or aggregated. Depending on cell type, may be found in thickened parts of the wlal, or in walls of farily uniform thickness.
Term
Plasmolysis
Definition
When a plant cell has lost water, and the protoplast shrinks. Hechtian strands connect the protoplast to the cell wall.
Term
Plasticity
Definition
A property of the primary cell wall. Becomes permanently deformed when subjected to change in shape or size.
Term
Plastid
Definition
A defining characteristic of plant cells. Also found in bacterial cells, but not in animal cells. Plant plastids have two membranes. Organelles with various shapes, often relating to function and maturity of the cell, including chloroplasts, amyloplasts, etioplasts, chromoplasts, and statoliths.
Term
Pleomorphic
Definition
Having more than one shape. Chloroplasts are pleomorphic.
Term
Pluripotent
Definition
Restricted in the number of cell types that it can produce; usually region or mother tissue specific.
Term
Point source of light
Definition
Appears as airy diffraction patterns or airy disks. Dissolve to form the image.
Term
Poison ivy
Definition
Has poisonous sap in stinging hairs, which gives a burning sensation. In the same family as cashew nut and mangoes. People who have sensitivity to poison ivy often also have allergic sensitivity to mango and cashew.
Term
Pollen tubes
Definition
Appear as thin threads. May have callose plugs.
Term
Post-mitotic growth zone
Definition
A zone of a root apex.
Term
Preparation of tissues
Definition
The first step of botanical microtechnique. Includes whole mounts, maceration, and sections.
Term
Pressure-flow hypothesis
Definition
Drives movement in the phloem. Dissolved photosynthates move passively along gradients of pressure and water potential.
Term
Primary cell wall (PCW)
Definition
The first visible cell wall layer to develop. Composed of a continuously interconnected fortifying system of aggregated cellulosic microfibrils. Results from simultaneous polymerization and crystallization of cellulose molecules. A dynamic, thin structure that can grow, stretch and change with the cell and its needs. It has plasticity, elasticity, and tensile strength. 25% cellulose, 25% hemicellulose, 35% pectin, 5% protein.
Term
Primary growth
Definition
Produces primary tissues, and relates to the development of a plant as a whole.
Term
Primary meristem
Definition
Meristems with initials that are direct descendants of embryonic cells. Produced by apical meristems. Form the basic structure of the plant: the primary tissues. Includes protoderm, ground meristem, and procambium.
Term
Primary phloem
Definition
A primary tissue of the vascular system. Produced by the procambium.
Term
Primary plant body
Definition
The outermost layer is the epidermis. However, secondary tissue may be added on top of the epidermis.
Term
Primary tissues
Definition
Produced by the primary meristem. Includes the epidermis, ground tissue, primary xylem, and primary phloem.
Term
Primary xylem
Definition
A primary tissue of the vascular system. Produced by the procambium. Includes protoxylem and metaxylem.
Term
Procambium
Definition
A primary meristem. A thin layer in the embryo and SAM. Forms primary xylem and primary phloem.
Term
Promeristems
Definition
Partly differentiated, but meristematic cells. Found in the central zone of the SAM, dividing infrequently; analogous to the quiescent centre. Includes the protoderm, procambium, and ground meristems.
Term
Propidium iodide
Definition
A vital stain used to stain nuclei. Excluded by living cells. Only enters dead cells, and can be used to indicate which cells are dead and which are living.
Term
Protoderm
Definition
A primary meristem. The first epidermal cells, found in the plant embryo. Produces the epidermis.
Term
Protoplast
Definition
The portion of the plant cell within the plasma membrane. The entire living plant cell, without the cell wall. When separated from the cell wall, it takes the shape of a sphere. The protoplast swells to fill the shape of the cell wall.
Term
Protoxylem
Definition
Primary xylem that develops before the metaxylem, usually before and during elongation of the axis. Forms narrower vessels and tracheids. Tracheids are generally smaller in diameter than metaxylem.
Term
Quiescent centre
Definition
Part of a root apex, where cell division is relatively infrequent.
Term
Radial longitudinal section
Definition
A section made parallel to the longitudinal axis, and through the radius of a plant organ.
Term
Rayleigh limig
Definition
The theoretical limit of resolution at a given wavelength of light.
Term
Resolution
Definition
The ability to resolve detail, distinguishing between two points in an image.
Term
Respiratory arrest
Definition
When a mitochondrion's energy is too high or too low. It can fuse with another mitochondrion to re-establish an energy gradient.
Term
Rhodamine 1,2,3
Definition
A fluorescent probe used to stain mitochondria.
Term
Ribosomes
Definition
Found in both plant and animal cells.
Term
Robert Hooke
Definition
Was a chair of the Royal Society of London for Improving Natural Knowledge. Was a teacher and an observer. First saw cells of cork tissue, and they reminded him of small cramped quarters, so he called them "cells".
Term
Root apical meristem (RAM)
Definition
The most active part of a seedling. If it cannot grow downwards, it will circle until it overcomes obstacles.
Term
Root cap columella
Definition
Part of a root apex.
Term
Root hair
Definition
Development includes cell specification, bulge formation, and extension.
Term
Ruthenium red
Definition
A botanical stain used to stain middle lamella.
Term
Sacred fig
Definition

Ficus religiosa

The leaves of this tree turn white, and leave hard tissue behind, forming a "skeletonized" leaf.

Term
Safranin
Definition
A botanical stain used to stain lignified cell walls.
Term
Salt gland
Definition
Found in halophytes. Regulates salt content of shoots. Have a large bladder containing a vacuole into which ions are secreted.
Term
Scanning electron microscope (SEM)
Definition
A microscope which uses electrons instead of light. Produces greyscale images. Creates a vacuum in the chamber; plant tissues can become dried out.
Term
Sclereids
Definition

Stone cells

Sclerenchyma cells that are thick, shorter than fibres, and variable in shape. Secondary cell wall is thick. May be living or dead when functional. Located throughout the plant. Provide mechanical support and protection. Includes astrosclereids, brachysclereids, macrosclereids, osteosclereids, and trichosclereids.

Term
Sclerenchyma
Definition
A cell type of ground tissue. Located in all plant organs. Provide mechanical support. Have a secondary cell wall, which is lignified and pitted. Narrow and elongated cells. Normally dead at maturity. Includes fibres and sclereids.
Term
Secondary cell wall (SCW)
Definition
Forms on the inside of the primary cell wall, after the cell stops growing. Stronger than primary cell wall. Some cells have multiple secondary cell wall layers, each with distinct characteristics. Usually develop in structural cells, and cells which transport water or sugars. Contributes to wood. It consists of cellulose, non-cellulosic polysaccharides, hemicellulose, and lignin. Secondary cell wall thickenings may be annular, annular/helical, double helical opposite curl or same curl, or pitted.
Term
Secondary growth
Definition
Additional growth that thickens the stem and root. Involves activity of the vascular cambium.
Term
Secondary meristems
Definition
Meristems with initials that originated from cells that had differentiated and then resumed meristematic activity. Add tissues to the plant body after the primary meristem. Includes cork cambium.
Term
Secondary xylem
Definition
Xylem which develops after the metaxylem.
Term
Sections
Definition
A way to prepare tissues. Used for complex, massive tissues. May be sectioned by hand using razor blades, or using embedded materials such as paraffin and a microtome, or freezing and a cryotome. Sections for ultrastructure use resin embedding. Includes transverse, radial longitudinal, tangential, and paradermal section. Can also include optical sectioning.
Term
Shoot apical meristem (SAM)
Definition
The apical meristem at the apex of the stem. A collection of stem cells just above the youngest leaf primordium. All primary tissues in the stem arise from the SAM. Can have tunica-corpus organization prior to flowering.
Term
Sieve area
Definition
Areas at the ends of sieve cells where pits are concentrated. Walls with pores of variable diameter, through which protoplasts of vertically or laterally adjoining sieve elements are connected. Found in sieve elements. Sieve cell sare adjoined to each other at their sieve areas.
Term
Sieve cell
Definition
A cell type of phloem tissue, found only in gymnosperms. A sieve element. Elongated and tapering in shape. Has primary cell wall in most species with pitted sieve areas, and callose often associated with walls and pores. Living at maturity. Lacks or has remnants of a nucleus at maturity. Lacks distinction between vacuole and cytoplasm; contains large amounts of tubular endoplasmic reticulum. Lacks P-protein. Functions in long distance transport of photosynthates and molecules.
Term
Sieve element (SE)
Definition
Refers to photosynthate-conducting cells and their modified parenchyma cells of the phloem. Lined with callose, which decreases when the phloem is active, re-establishing protoplast continuity. Vary in thickness, but are thicker than parenchyma. In fresh sections they appear nacreous. Connected by sieve areas or sieve plates. Includes sieve cells, albuminous cells, sieve-tube elements, and companion cells.
Term
Sieve element protoplast (SEP)
Definition
Has sequential changes during development; nuclear degeneration, stacking of endoplasmic reticulum, protein and enzyme accumulation, and disappearance of dictysomes and ribosomes. Starch and protein-accumulating plastids and mitochondria remain. Plasma membrane persists, but the tonoplast breaks down. Forms P-protein.
Term
Sieve plate
Definition
Areas at the ends of sieve-tube elements where there are noticeably larger pores than on radial or tangential walls. The dividing barriers between sieve tube elements.
Term
Sieve tube
Definition
Several sieve-tube elements in a vertical series. A photosynthate-conduction pipe.
Term
Sieve-tube element
Definition

Sieve tube member

A cell type of phloem tissue, found only in angiosperms. A sieve element. Elongated shape. Has primary cell wall with sieve areas on lateral walls and sieve plates on end walls. Callose is often associated with walls and pores. Living at maturity. Lacks or has remnants of a nucleus at maturity. Contains P-protein, except for some monocots. Line up to form the sieve tube. Functions in long distance transport of photosynthates and signalling molecules.

Term
Sink tissue
Definition
Tissue which requires sugars. Companion cells unload sugar from the phloem. In the summer storage organs such as roots are sink tissues, and in the spring growing leaf buds are sink tissues.
Term
Sleeping stoma
Definition
A closed stoma.
Term
Solanaceae
Definition
A family of plants which have chemicals harmful to insects, produced in glandular trichomes. Includes tobacco, jimsonweed, and potatoes.
Term
Source-sink relationship
Definition
Any cell can be a sink or a soruce, depending on the relative sugar concentration. Even individual chloroplasts within a cell can be sources or sinks, due to unequal light incidence.
Term
Source tissue
Definition
Tissue which produces sugars. Companion cells load sugar into the phloem. In the summer leaves are source tissues, and in the spring storage organs such as roots are source tissues.
Term
Sphere
Definition
The smallest surface area among all surfaces enclosing a given volume. A protoplast without a cell wall takes on the shape of a sphere because surface tension minimizes surface area.
Term
Stain
Definition
Increases contrast, and can label specific components. Unlike a dye, it is temporary.
Term
Starch
Definition
A polysaccharide. 30% of sugar produced by a plant is converted into starch. Includes transient starch and storage starch. May be stained with iodine-potassium iodide.
Term
Starch sheath
Definition
An inner layer of the cortex that is high in starch.
Term
Statolith
Definition
A plastid found at the tip of roots. Govern direction of growth by sensing gravity. Found in statocyte cells.
Term
Stele
Definition
The central part of the root of vascular plants. A rigid structure enclosed by endodermis.
Term
Stem
Definition
Important for the structure and physiological support of above-ground organs including leaves, flowers, and fruits. Contain structural ground tissues that add girth and strength, as well as vascular tissues.
Term
Stinging hairs
Definition
A defense mechanism. Emergences of epidermal and sub-epidermal layers. Consists of a spherical tip, a neck, a shaft, and a base containing poisonous fluid. The tip gets broken off, and the broken neck acts as a needle that injects poison into the animal. Found on poison ivy and stinging nettle.
Term
Stinging nettle
Definition
Has stinging hairs, and pink flowers.
Term
Stoma
Definition

"Mouth"

The pore between two guard cells, through which gas exchange occurs.

Term
Stomatal complex
Definition
Two guard cells and the stoma.
Term
Stomatal crypt
Definition
A depression in the epidermis where stomatal complexes may be found. Protected by hairs. Common in plants in dry areas. Found on oleander leaves.
Term
Stomatal spacing
Definition
Patterns of stomatal spacing can change in response to environmental conditions. Controlled by signalling.
Term
Stomatal wax chimney
Definition
A formation of epicuticular wax found in some xerophytes and succulents.
Term
Storage starch
Definition
Starch that is stored in tubers, or other storage organs. The plant may store energy in the form of starch for winter.
Term
Stromules
Definition
Stroma filled tubules that emerge from plastids, including chloroplasts.
Term
Suberin
Definition
A component of the cell wall. Insoluble lipid polymers. A waxy, waterproof substance that forms a protective surface matrix in which waxes are embedded to prevent loss of water. A major component of secondary protective tissues such as cork and endodermis. Restricts apoplastic water movement. May be stained with berberine hemisulphate or Sudan red. Found in the Casparian strip.
Term
Succulent
Definition
Spongy, fat, turgid tissue with juicy flesh. Macerations are used to visualize succulent structures.
Term
Sudan III/IV
Definition
A botanical stain used to stain cuticle. Stain lipids red or orange-red. Suberin and the cuticle stain orange-red.
Term
Sudan red
Definition
A botanical stain used to stain suberinized cell walls.
Term
Sudan red 7B
Definition
A botanical stain which stains lipids red or orange-red.
Term
Sugar
Definition
A natural antifreeze; lowers the freezing point of water. Sugary water is present in the xylem of trees during the winter, to protect against the cold. Sugar always travels down its concentration gradient in the phloem, from source to sink.
Term
Sundew
Definition
A plant with sticky colleters it uses to catch insects. The droplets look radiant in the sun. Includes Drosera rotundifolia and D. anglica.
Term
Surface tension
Definition
An effect within the surface layer of a liquid which causes the layer to behave as an elastic sheet.
Term
Symplastic
Definition

"Inside plasma"

Transport of water and solutes from cell to cell through plasmodesmata and protoplasts.

Term
SYTO dyes
Definition

SYTO 13/25

A nucleic acid stain used to stain the nucleus. This type of dye can be expensive, but it produces many colours.

Term
Tangential section
Definition
A longitudinal section made at a right angle to the radius of a plant organ.
Term
Telophase
Definition
A new cell wall forms at the former metaphase plate, aided by the phragmoplast.
Term
Tensile strength
Definition
A property of the primary cell wall. The force require to pull something to the point where it breaks. Has practical implications, and is important for woody or climbing plants.
Term
Thallus
Definition
A sac-like structure that is flat and has pigment, or is flat and close to the ground. Whole mounts may be used to visualize thallic structures.
Term
Time lapse imaging
Definition
Provides a record of cellular activities during development. Extends anatomical studies to an understanding of plant development.
Term
Tip growth
Definition
A type of growth in epidermal cells. Produces collet and root hairs. Growth from one point on the surface of the cell, forming a hair. In some cells hairs can be forked.
Term
Tissue
Definition
Composed of multiple cell types.
Term
Toluidine blue O (TBO)
Definition
A general basic stain, water soluble, which is metachromatic. Produces different colours depending on the binding site in the tissue, and pH of the solution. Unmodified cell walls stains purple. Pectin stains pink to reddish purple. Lignin stains blue to green. At high pH nuclei stain green-blue.
Term
Tomogram
Definition
Creates a 3D image by putting together several layers of scans.
Term
Tonoplast
Definition
The membrane of the large vacuole. May be stained with FM 4-64.
Term
Totipotent
Definition

"Total potential"

Having the ability to produce the entire spectrum of cell types, even a complete plant.

Term
Tracheary elements
Definition
Water-conducting cells of the xylem. Elongated, thick-walled and lignified, dead at maturity. Can have pitted or helical secondary cell walls. Able to withstand functional tensions to aid in cohesion-tension theory of water movement. Includes tracheids and vessel members.
Term
Tracheid
Definition
A cell type of xylem tissue. Found in gymnosperms and seedless vascular plants, and some angiosperms. A tracheary element. Elongated and tapering in shape, pointed at both ends. Has primary and secondary cell wall, lignified with pits, but not perforations. Lack end walls, and are arranged for staggered sideways water transfer. Water passes through pit membranes. Similar-looking to fibres. Tracheids of conifers usually have bordered pits with a porous margo and thickened torus in the pit membrane, important for isolating air embolisms. Dead when functional. Function in water conduction.
Term
Transdifferentiation
Definition
Differentiation of a plant cell without division.
Term
Transfer cells
Definition
Cells that are wall ingrowths of some companion cells.
Term
Transient starch
Definition
Starch that is made throughout the day. There is more transient starch in a plant in the daytime than there is at nighttime.
Term
Transmission electron microscopy (TEM)
Definition
A microscope with which you can see the inner structures of the cell.
Term
Transmitted light
Definition
Light from below which the specimen blocks or allows through.
Term
Transpiration
Definition
Evaporative loss of water through stomata. Drives transpirational pull.
Term
Transpirational pull
Definition
One of the main forces that drives water movement in the xylem. Results from transpiration of water from leaves.
Term
Transverse section
Definition

Cross section

A section made at a right angle to the longitudinal axis of a plant organ.

Term
Trichoblast
Definition
A cell which gives rise to a root hair. Rows alternate between trichoblasts and atrichoblasts.
Term
Trichome
Definition
A hair on an aerial part of the plant. Produced by diffuse growth of epidermal cells. A single leaf may have trichomes at different stages of development. Protect the plant from insects. In Arabidopsis, trichomes are single-celled and three-pronged. Incldues glandular trichomes and peltate trichomes.
Term
Trichosclereid
Definition
A branched sclereid with slender hair-like projections into intercellular spaces, some reaching several milimeters in length.
Term
Trypan blue
Definition
A vital stain excluded by living cells.
Term
Tunica
Definition
The two outer layers of the SAM. Divide anticlinally, maintaining the surface area of the SAM as the stem elongates.
Term
Tylose
Definition
Induced outgrowths from paratracheal parenchyma cells. Block xylem vessels as a step in wound healing. Parenchyma cells that invade the lumen of tracheary elements through a pit. A special wall layer is placed on teh side of the parenchyma cell, forming the tylose. Occurs when the plant is wounded or has a fungal infection.
Term
University
Definition
Comes from the word for "expand". At university you do not just learn, you expand existing knowledge and produce new content.
Term
UV light
Definition
It is harmful to living cells, but can be used on dead cells when doing fluorescence microscopy.
Term
Vacuole
Definition
A defining characteristic of plant cells. Very rarely found in animal cells. Highly variable in size, shape, and function. A large vesicle that can fill over 95% of plant cell volume, surrounded by the tonoplast. Filled with metabolites and other substances. Can occur in patches of membrane and tubules in living cells.
Term
Valley of flowers
Definition
A valley in the Himalayas. In May and June, there are so many flowers that they cover the hills; there is a high level of plant diversity. Shows that vascular plants can live high above sea level.
Term
Vascular bundle
Definition

Fascicle

Discrete strands of phloem and xylem, located in shoot organs. Sheathed by one or more layers of bundle sheath cells made of parenchyma and/or sclerenchyma. Types of vascular bundles include collateral, bicollateral, amphicribal, and amphivasal.

Term
Vascular plants
Definition

Higher plants

Plants that have a vascular system. The vascular system allows them to rise above the water level and live on land.

Term
Vascular system
Definition
A system of cells and tissues dedicated to transporting water and dissolved minerals. Vascular differentiation starts in the embryo. New vasculature develops continuously from derivatives of meristems as the plant grows. Tissues include phloem and xylem.
Term
Velamen
Definition
A multiseriate layer of the epidermis of aerophyte orchids. Allows aerial roots to absorb water from the air or rain. The tips of roots may be green.
Term
Vessel
Definition
Vessel member cells in a vertical series. More efficient water conduits than tracheids. End walls are perforate. The wider and longer a vessel is, the higher its hydraulic conductivity. Water columns are susceptible to cavitation and embolisms.
Term
Vessel member
Definition

Vessel element

A cell type of xylem tissue. Found only in angiosperms. A tracheary element. Elongated in shape, but shorter and wider than a tracheid. Constitute a vessel. Has primary and secondary cell wall, lignified with pits and perforations, and a perforation plate on end walls. Has longitudinal files connected through pores; forming chains of cells. Dead when functional. Functions in water conduction.

Term
Visible light
Definition
Light between the wavelengths 400 nm and 700 nm.
Term
Vital stain
Definition
A stain used on living cells. Must be able to permeate the living cell membrane.
Term
Volvox
Definition
A colony of algae that forms a sphere. New volvox colonies form inside and are released when the parent colony breaks apart.
Term
Water cohesion
Definition
The sticking together of water molecules.
Term
Wavelength
Definition
The wavelength of light used to visualize a specimen is a major factor in the degree of resolution of the microscope. Shorter wavelengths can resolve details to a greater degree than longer wavelengths.
Term
Wax
Definition
Synthesized from elongation and modification of fatty acids. Hydrophobic. Some waxes are deposited as continuous films or crusts, others as filaments, ribbons, dendritic structures, and platelets. Includes epicuticular wax.
Term
Whole mount
Definition
A way to prepare tissues. Used for unicellular, filamentous, or thalloid forms.
Term
Wood
Definition
Xylem of trees. It can last for centuries; there are very old buildings made of wood, and some trees are up to 1,500 years old.
Term
Wound-induced meristem
Definition
A mechanism by which differentiated plant cells can be made into meristems.
Term
Xylem
Definition

"Wood"

A tissue of the vascular system. Transports water throughout the plant, according to cohesion-tension theory. Cells have thin primary cell walls and thick, lignified secondary cell walls, making tissue hydrophilic and mechanically strong. Except for parenchyma, cells are dead when functioning, lacking protoplasts. Cell types include tracheids, vessel members, fibres, and parenchyma.

Term
Zonation
Definition
Zones form in the tip of growing root hairs. Tip growth is occurring here. There is a clear zone, a vesicle rich zone, in an apical region and subapical region.
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