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Photosynthesis 2
The Calvin Cycle
12
Biochemistry
Undergraduate 4
04/13/2011

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Term
RuBisCo
Definition
Ribulose 1,5 Bisphosphate carboxylase oxidase
Term
RuBisCo facts
Definition
most prevalent protein on Earth. very inefficient enzyme b/c of its slow turnover rate and competing oxidase reaction. Uses Mg ion.
Term
RuBisCo reaction
Definition
RuBP(5C) + CO2(1C) -> (6C intermed.) -> 2 x 3-Phosphoglycerate(3C)
Term
RuBisCo location
Definition
stroma of the chloroplast/outer thylakoid membrane
Term
3 stages of the calvin cycle
Definition
fixation, reduction, regeneration
Term
magnesium
Definition
divalent cation stabilizes the enediolate intermediate.
Term
oxidase reaction
Definition
photorespiration. O2 forms hyperperoxide intermediate. end up with one 3-phosphoglycerate and one phosphoglycolate. phosphoglycolate is not easily metabolised.
Term
glyceraldehyde-3-phosphate dehydrogenase
Definition
2 forms: one in cytosol (us NADH;gluconeogenesis) and one in chloroplast (use NADPH; calvin cycle).
Term
carbon fixation enzymes using ATP and NADPH
Definition

ATP: phosphoglycerate kinase.

NADPH: GAP dehydrogenase

Term
2 fates for fixed carbohydrate
Definition

6 GAP produced: 5 go to stage 3 of calvin cycle, regeneration - make ribulose 1,5 BP.

1 goes to sugar synthesis (starch)

Term
percent of light energy on leaf used to fix carbon
Definition
5%
Term
cost to fix one molecule of CO2
Definition
3 ATP 2 NADPH. takes 6 turns to make a 6C molecule
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