# in order: first

test: blood cultures specimen:whole blood

additives: SPS to prevent phagocytosis invert/notes: n/a

# in order: second

test: coagulation

specimen: plasma

additives: sodium citrate invert/notes: 3-4x

# in order: third

test:chemistry,blood bank, serology

specimen: serum

additives: clot activators, none in glass

invert/notes: 5x, takes 60 minutes to clot, serum seperated by centrigugation after clotting

# in order: fourth

test: most chemistry tests

specimen: serum

additives: clot activators, polymer gel

invert/notes: 5x, also called tiger-speckled, jungle top, stat tubes. Clotting requires minimum 30 mins.

# in order: fifth

test: stat chemistry, ammonia, electrolytes, ABGs (depends on the heparin additives)

specimen:plasma

additives:soduim heparin, lithium heparin or ammonium heparin.

invert/notes: 8x

# in order: sixth

test: CBCs, sedimentation rate

specimen: whole blood

additives: EDTA 

invert/notes: 8x

# in order: seventh

test: glucose, lactic acid, FBS, blood alcohol levels

specimen:plasma

additives: antiglycolytic agent (iodoacetate or soduim fluoride) 

invert/notes: 8x, iodoacetate preserves glucose for 24 hours, whereas soduim fluoride 3 days.