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Oral Biofilms chapter 6
periodontology
57
Dentistry
Undergraduate 3
06/02/2014

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Term
inocuous
Definition
species of bacteria not harmful
Term
pathogenic
Definition
capability of causing disease in humans
Term
replication
Definition
replication happens quickly
Term
characteristics of gram +
Definition

thick, single cell wall

 

stains purple

Term
characteristics of gram -
Definition

double cell walls

 

does not stain

 

plays a role in tissue destruction  (anaerobic)

Term
aerobic
Definition
needs oxygen
Term
anaerobic
Definition
cannot live in oxygen
Term
obligate anaerobic
Definition
cannot tolerate free oxygen
Term
facultative anaerobic
Definition
can exist with or without oxygen
Term

non motile

 

facultative

 

cocci and rods

Definition
gram +
Term
perio pathogens
Definition

T-forcythes (gram -)

 

A A  (gram -)

 

P. Ginivalis (gram -)

 

fusobacterium nucleatum (gram -)

 

all are non motile

Term
what is the purpose of fluid channels
Definition
move nutrients and bacterial products throughout biofilm structure
Term
quorum sensing
Definition
communication between bacterial colonies
Term
plaque to bone is never less than ___mm. and never greater than_____mm.
Definition

never less than .5mm

nor more than 2.7mm

Term
what are bacterial virulence factors?
Definition

invasion of body tissue

 

endotoxin peptides

 

enzymes

 

exotoxin, leukotoxins

Term
peptides
Definition
a short chain of amino acids found in the cell membrane that controls the transport of molecules out of the cells
Term
what do T cells do?
Definition
lock onto peptides and allert immune system of invasion
Term
in health what are the % of gram + and gram -?
Definition

75% gram + in health

 

25%gram -

Term
lifecyle of biofilm
Definition

attachment

 

growth

 

detachment

Term
what is the form of bacteria that shows up after 6 hours when supragingival plaque is formed?
Definition
actinomyces streptococci
Term
what are the 1st layer of nonpathogenic gram + that are normal flora
Definition
S. mitis, s. oralis, and A. vicosus
Term
how are enzymes produced
Definition
by peptides
Term
what are the enzymes in mouth
Definition

protease, collagenase, and elastase

 

help with invasion of tissues

 

increase permeability,breakdown collagen, promote apical migration, widen intercellular spaces, diminish ability of immunoglobins to defend host.

Term
exotoxins
Definition

proteins released from the bacterial cell that cause tissue damage.

 

 

IE)  leukotoxin is released from pathogen AA to destroy leukocytes.!!!!!

Term
dormant bacteria
Definition
inactive in order to survive adverse conditions
Term
how to control plaque biofilm
Definition

physical disruption

 

toothbrushing and flossing

 

instrumentation

Term

AA

 

 

motile or nonmotile?

 

gram + or -?

 

what does it look like?

 

what tissue is it found in?

Definition

Actinobacillus actinomycetemocomtians

 

GRAM -

 

non motile

 

small short rods, straight or curved with round ends

 

in connective tissue!!!!

Term
AA is associated with what types of perio?
Definition
aggressive perio and generalized perio
Term
what is the lipopolysachharide / endotoxin formed by AA that forms pores in pmns (which die due to osmotic pressure)?
Definition
leukotoxins form pores in pmns.
Term
NUP-trenchmouth
Definition
found in soldiers, bad diet, bad Oral hygiene
Term
Provotella intermedia
Definition

a gram - nonmotile pathogen

 

round ended rods

 

associated with NUP, pregnancy gingivitis, and perio abcess.

 

less virulent that P. gingivalis

Term

fusobacterium nucleatum a gram - nonmotile pathogen

 

 

tell me about it...

Definition

cigar shaped bacillus with pointed ends.

 

triggers release of cytokines and O2 from leukocytes.

 

can co-aggregate with most other Micro organisms of oral cavity so it is thought to be the "bridge" in plaque formation between early and late

Term

Prophyromonas gingivalis

 

 

or p. gingivalis

Definition

gram - non motile

 

obligate anarobic rods

 

black from IRON

 

lives in connective tissue and in JE and alveolar bone

Term
what mediates adhesion and capsule and defends against phagocytosis in P. gingivalis?
Definition

fimbriae

 

 

it produces proteases and degradation of host cells collagenase inhibitors.

 

 

P. gingivalis does not allow pmns for healing

Term
Treponema Denticola
Definition

spirochetes, anaerobic

 

associated with early onset perio, NUG, acute pericornitis

 

 

can travel through viscous fluid through connective tissue!!

Invades epithelial and connective tissue!!!!

 

produces enzymes that kill IgA, IgG, and IgM

Term
Bacteroides forsythus
Definition

gram - non motile

 

obligate anaerobic

 

deep pockets and aggressive perio

 

produces enzymes which cause death to immunoglobulins and cell death

Term
what type of microbes might you find in gingiva in health?
Definition

gram positive rods and short rods

 

in health

Term
what types of biofilm might you find in gingivits?
Definition

fusobacterium nucleatum

 

veillonella parvula

 

actinomyces viscous

 

P. intermedium

in gingivitis
Term
what types of biofilm might you find in adult periodontitis?
Definition

-p. gingivalis

 

-A. A.

 

-P. intermedia

 

-b. forsythis

 

-f. nucleatum

 

-eikinella corrodens

 

-c. rectus

 

-treponema, sp

in adult perio
Term
what biofilm might be present in refractory adult perio?
Definition

-p. gingivalis

-A.A.

-P. intermedia

-b. forsythus

-F. nucleatum

-C. rectus

-P. micros

in refractory perio
Term
what biofilms might be present in generalized and localized juvenile perio?
Definition

P. gingivalis

 

A. A.

 

Prevotella. sp

juvenile perio
Term
what biofilm may be present in prepubertal gingivitis?
Definition

b. forsythus

 

f. nucleatum

 

c. rectus

prepubertal gingivitis
Term
what biofilm may be present in NUG
Definition

p. intermedia

 

F. nucleatum

 

borrelia vincentii

 

spirochetes

in NUG
Term
what biofilms may be present in HIV?
Definition

P. gingivalis

 

A.A.

 

P. intermedia

 

f. nucleatum

 

C. rectus

 

Candida albicans

HIV patients
Term
what microorganisms may be found in rapid progressive perio?
Definition

P. gingivalis

 

A.A.

 

B. forsythis

 

p. intermedia

 

C. rectus

rapid progressive perio
Term
what may be found in pregnancy gingivitis as far as microorganisms?
Definition
prevotella intermedia
for pregnancy
Term
what are the most frequently implicated microorganisms?
Definition

p. gingivalis

 

A.A.

 

P. intermedia

Term
what color of socranskys complex is the worst
Definition

red is worst

 

then orange is next worst

Term
what MO are involved in socranskys red complex?
Definition
P. gingivalis, T. Forsythia and T. denticola
Term
what  MO are involved in socranskys orange complex?
Definition

p. intermedia,

p. nigrescens,

p. micros,

f. nucleatum,

borrellia vincentii,

polymorphum,

f. peridonticum

Term
what organisms are found to have the ability to invade oral tissues?
Definition

A.A.

 

P. gingivalis

 

F. nucleatum

 

Treponema 

Term
what is the bacteria first to be deposited on teh tooth in bacterial plaque formation?
Definition
gram positive cocci
Term
what is the order of organisms in undisturbed plaque biofilm?
Definition

s. mutans (1-2 days)

 

filamentous rods and fusobacterium (4-7 days)

 

vibrios and spirochetes (days 7-14)

Term
puberty gingivalsi and pregnancy gingivitis have an increase in what bacteria?
Definition
p. intermedia
Term
what is a primary microbe often found in NUG?
Definition
p. intermedia
Term
what is a gram - non motile pathogen found in small numbers in healthy peridontium and in large numbers in recurrent disease sites with deep periodontal pockets?
Definition
porphyromonas gingivalis.
Term
Some micro organisms with less significance are
Definition

b. forsythus

 

fusobacterium sp

 

peptostreptococcus micros

campylobacter rectus

treponmena denticola

enteric rods

streptococcus sp

actinomyces sp

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