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OCTC-Micro-Chapter 19
OCTC-McElroy's class Summer 2013
197
Microbiology
Undergraduate 1
06/17/2013

Additional Microbiology Flashcards

 


 

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Term
Pathogenic Gram-Positive Cocci and Bacilli
Definition
Stain purple when gram-stained
Term
Staphylococcus is a _____ member of every human’s microbiota.
Definition
Normal
Term
Staphylococcus is located:
Definition
In nasopharynx
On skin
Colonizes the infant w/in hours
Term
Staphylococcus found in hospitals in
Definition
Nurseries and surgical wards.
Term
Staphylococci live and reproduce on
Definition
almost every square inch of human skin
Term
Staphpylococcus can be ______ pathogens causing anywhere from minor to life threatening diseases
Definition
opportunistic
Term
Staphylococcus genus name comes from Greek term staphle meaning ______
Definition
“bunches of grapes”
Term
Coccus (pl.cocci) =
Definition
spherical–shaped bacterial cell
Term
staph =
Definition
staff or group (Cluster like grapes)
Term
Staphylococcus colonies appear
Definition
cream colored, white to light gold, and “buttery-looking”
Term
Staphylococcus is Gram _____
Definition
positive cocci, nonmotile, facultative anaerobes
Term
Staphylococcus grows best when _____ present, but can continue to grow under ____ conditions.
Definition
O2, anaerobic
Term
Staphylococcus is _____-tolerant
Definition
salt
Term
Staphylococcus tolerates the ____ present on human skin
Definition
salt
Term
Staphylococcus is tolerant of desiccation (capsule). What does this mean?
Definition
allows survival on environmental surfaces such as fomites
Object inadvertently used to transfer pathogen to new host: needle or coin
Term
Staphylococci are catalase positive or negative?
Definition
positive
Term
Catalase Converts H202, (hydrogen peroxide) Into_____ and ____?
Definition
H20 and 02
Term
Staphylococci can disarm neutrophil’s and macrophage’s H2O2 with ______?
Definition
catalase
Term
_______ is secreted by neutrophils and macrophages to kill bacteria
Definition
Hydrogen peroxide
Term
To test for catalase:
Definition
Inoculating loop is rubbed across a colony of Staphylococcus and mixed with H202 on a slide.
If bubbles appear, the enzyme catalase must be present.
So Staphylococcus is catalyst positive
Term
Staphylococcal infections range from the
Definition
trivial to the rapidly fatal
Term
Are Staphylococcal infections easy to treat?
Definition
No, They can be difficult to treat
Term
Why are Staphylococcal infections difficult to treat?
Definition
Because staphylococci can acquire antibiotic resistance
Term
“Staph” infections result when staphylococci
Definition
breach the body’s physical barriers.
Term
Entry of only a few hundred bacteria (ID) can result in
Definition
disease
Term
Staphylococci are ______ in nature with about a _____ species part of our human flora
Definition
ubiquitous, dozen
Term
How many species are commonly associated with staphylococcal diseases in humans?
Definition
2
Term
What are the 2 species commonly associated with staphylococcal diseases in humans?
Definition
1.Staphylococcus aureus is one of the most common causes of infections
2.Less virulent,opportunist Staphylococcus epidermis may cause prosthetic implant infections
Term
Staphylococcus aureus is one of the most common causes of
Definition
infections
Term
Less virulent, opportunist Staphylococcus epidermis may cause
Definition
prosthetic implant infections
Term
Staphylococcus epidermidis is the______ of human skin
Definition
Normal microbiota
Term
Staphylococcus epidermidis is the normal microbiota of human skin that can cause ?
Definition
opportunistic infections to immunocompromised patients or when introduced into parts of body
Term
Staphylococcus aureus is located on
Definition
skin, nares, mucous membranes (pharynx and vagina)
Term
What pigment is Staphylococcus aureus on sheep blood agar?
Definition
Gold pigment
Term
Pathogenicity of Staphylococus (3 things)
Definition
1.Cell wall structures that prevent phagocytosis
2. Enzymes
3. Production of toxins
Term
Cell wall structures that prevent phagocytosis (3 things)
Definition
a. Protein A
b. Bound coagulase
c. Capsule/slime layer
Term
2. Enzymes (5 things)
Definition
a. Coagulase
b. Staphylokinase
c. Hyaluronidase
d. Lipase
e. B-lactamses
Term
3. Toxins (4 things)
Definition
a. Cytolytic toxins
b. Exfoliative toxins
c. Toxic-shock-syndrome (TSS) toxin
d. Enterotoxins
Term
Staphylococcus aureus has Protein A which causes_____to bind upside-down _____ antigen. This _____ opponization and ________.
Definition
antibody stems,S. aureus
Inhibits, phagocytosis
Term
Staphylococcus aureus has ________ that converts fibrinogen into ______ that form clots around pathogen.
Definition
enzyme coagulase, fibrin
Term
Fibrin clots ______ Staphylococcus aureus from phagocytic cells
Definition
hide
Term
Staphylococcus aureus forms slime layer/capsule that prevents:
Definition
Phagocytosis and Dessiccation
Term
Slime layer facilitates attachment of
Staphylococcus to artificial surfaces such as (5 things)
Definition
catheters, shunts, artificial heart valves and joints
Term
Coagulase triggers fibrin clotting, which hides the _______
Definition
Staphylococcus aureus
Term
Staphylokinase
Definition
Dissolves fibrin threads in clots, allowing S.aureus to free itself from clots and spread to new location
Term
Hyaluronidase
Definition
Breaks down hyaluronic acid, enabling S. aureus spread deeper between cells
Term
Lipases =
Definition
digest lipids allowing Staphylococcus aureus to grow on surface of skin and sebaceous glands
Term
B-lactamase =
Definition
penicillinase now in 90% of S. aureus strains and thus resistant to penicillin and cephalosporins
Term
Cytolytic toxins Disrupts the ______ ______ ______of a variety of cells such as
Definition
mammalian cytoplasmic membrane
Heart, skeletal muscle, renal cells, platelets
Term
Hemolysins damage
Definition
RBCs
Term
Leukocidin lyses
Definition
Leukocytes, Macrophages, and neutrophils
Term
Leukocidin provides S. aureus ______ from _____.
Definition
protection from phagocytosis
Term
Exfoliative toxins = ET causes
Definition
Exofoliation
Term
Exfoliative toxin dissolves
Definition
desmosomes
The patient’s epidermal layer of skin to slough off
Term
Toxic-shock-syndrome toxin symptoms
Definition
High fever, rash, peeling of skin, vasodilation, drop in BP
Term
Staphylococcus aureus produces enterotoxin proteins which in turn stimulate which 4 things?
Definition
Intestinal muscle contractions
Nausea
Intense vomiting
Associated with staphylococcal food poisoning
Term
Staphylococcus aureus Enterotoxins are ____ stable
Definition
heat
Term
Heat does not denature_____ protein
Definition
enterotoxin
Term
cover dish dinner; take home leftovers; reheat = zap in microwave. This process Does not _______the Enterotoxin
Definition
denature
Term
Staphylococcal Diseases categorized as which 3 things?
Definition
1. Noninvasive Disease
2. Cutaneous Disease
3. Systemic Disease
Term
1. Noninvasive Disease
Definition
Food poisoning
Ingestion of Staphylococcus aureus contaminated food
Term
Ingestion of Staphylococcus aureus contaminated food Bacteria grow on food and secretes
Definition
Enterotoxin that is heat stable
Term
Common cause of food poisoning?
Definition
Staphylococcus aureus
Term
Food poisoning is caused by ______ rather than by ______ of the bacteria
Definition
enterotoxin, invasion
Term
Food must remain at ________for several hours for bacteria to grow, reproduce, and produce toxin
Definition
room temperature
Term
Enterotoxin causes ____, ____, ____ for how long?
Definition
nausea, vomiting, diarrhea for 24 hours
Term
Reheating may kill ______ bacteria but does not inactivate _______.
Definition
S. aureus, enterotoxin
Term
2. Cutaneous Disease
Various skin conditions: name 6
Definition
Scalded skin syndrome
Impetigo
Folliculitis
Sty
Furuncles
Carbuncle
Term
Staphylococcus aureus causes localized _______(pus filled lesions)lesions
Definition
pyogenic
Term
Pyogenic =
Definition
pus filled lesions
Term
Scalded skin syndrome =
Exfoliative toxin causes: name 4 things
Definition
Reddening of skin
Large blisters
Epidermis peels off in two days
Subject to secondary bacterial infection
Term
Scalded skin syndrome = Exfoliative toxin Usually affects ______ of severed umbilicus & Older children with ______.
Definition
neonates, skin infections
Term
Staphylococcus aureus causes 80% of
Definition
impetigo
Term
impetigo is what and occurs on who?
Definition
Small, flattened, red patches on face, limbs of children whose immune system is not fully developed
Term
impetigo mostly occurs on _____ as pus filled vesicles that crust over and become honey colored, wet, flaky. The pus is filled with ____ and ____
Definition
face, S. aureus and WBC
Term
Folliculitis =
Definition
Infection of hair follicle
Term
Folliculitis Becomes red, swollen, and pus filled. When it occurs at base of eye in eyelash follicle it is called a
Definition
Sty
Term
Furuncle = boil
Penetrates into the ______?
Definition
Large, painful, raised nodular extension of folliculitis to surrounding tissue.
Penetrates into the subcutaneous layer
Term
Carbuncle =
Definition
several furuncles coalesce
Term
Carbuncle
Definition
Larger, deeper, more painful
May have to be surgically drained
Can be the size of baseball
Term
Carbuncle May cause____ and _____ as S. aureus spreads into underlying tissues
may need ______.
Definition
fever, chills, antibiotics
Term
S. aureus can cause a variety of potentially fatal _____ _____ When introduced to deeper tissues of the body such as ___, ____, ____, and ____.
Definition
systemic infections
Blood, heart, lungs, and bones
Term
3. Systemic Disease 6 things
Definition
TSS
Pneumonia
Bacteremia
Endocarditis
Osteomyelitis
Empyema
Term
Staphylococcus aureus produce _____ toxin
Definition
TSS
Term
Staphylococcus aureus can grow in a _____ or _____ vagina and produce the toxin and cause______.
Definition
wound,abraded
Toxic shock syndrome
Term
can TSS occur in both males and females?
Definition
Yes
Term
Toxic Shock Syndrome Toxin causes:
Definition
Fever
Vomiting and Diarrhea
Red rash
Loss of sheets of skin
Low BP, shock
Multi-organ failure due to shock
Term
TSS fatal in ____% cases. Why?
Definition
5%, When BP drops so low O2 cannot be carried to vital organs and causes shock, death
Term
Staphylococcus aureus in blood is ______ and Accounts for half of all ______ infections
Definition
Bacteremia , noscomial
Term
Staphylococcus aureus Bacteremia Causes:
Definition
Furuncles
Vaginal infections
Infected surgical wounds
Term
Hospitalized patients with chronic disease that contract S. aureus bacteremia have a
Definition
high mortality rate
Term
Staphylococcus aureus = Endocarditis
Definition
Attacks lining of heart and valves
Term
Endocarditis is one of the consequences of _______.
Definition
staphylococcal bacteremia
Term
Staphylococcus aureus = Endocarditis
Symptoms are _____ and ______.
Definition
nonspecific, flulike
Term
Staphylococcus aureus = Endocarditis
Blood pumped from heart ______ which in turn makes the ____ drop.
50% patients do not
Definition
drops, BP, survive
Term
Staphylococcus aureus in blood invades _____ causing______.
Definition
lungs, pneumonia
Term
Mortality rate for staphylococcal pneumonia is _____%.
Definition
50%.
Term
Staphylococcal pneumonia known to occur as secondary infection to
Definition
influenza
Term
Staphylococcal pneumonia Particularly occurs in _____ and_______ patients
Definition
infants,cystic fibrosis
Term
In 10% of patients with Staphylococcal pneumonia… The fluid filling the alveoli is _____, this condition is called ______.
Definition
pus, empyema
Term
When Staphpylococcus aureusInvades a bone, it causes
Definition
Staphylococcal osteomyelitis
Term
Staphylococcal osteomyelitis Signs and symptoms are: Inflammation of
Definition
Bone marrow, diaphysis and periosteum
Fever, Chills, Swelling and pain
(Brake hip and get stahpylococcus aureus infection in bone)
Term
Diagnosis of Staphylococcus Infections
Definition
Detection of Gram-positive bacteria
Isolated from pus, blood, or other fluids
Term
Treatment: ______ is the drug of choice to treat staphylococcal infections.
Definition
Methicillin
Term
Treatment:Since (1962) 1987 have:
MRSA =
Definition
methicillin-resistant Staphylococcus aureus
Term
Treatment: Since 2002 have:
VRSA =
Definition
vancomycin resistant S. aureus
Term
Prevention = some humans are carriers
Definition
Hand antisepsis
Proper cleansing of wounds and surgical openings
Aseptic use of catheters or indwelling needles
Appropriate use of antiseptics
Term
The Genus Streptococcus Is a diverse group of
Definition
Gram positive cocci
Term
Streptococcus can be arranged in ____ or ______
Definition
Pairs or chains
Term
Streptococcus are arranged in pairs called
Definition
diplococci
Term
Streptococcus are Arranged in chains like a strip of button
candy called
Definition
streptococci
Term
Streptococcus is Catalase _____
Definition
negative
Term
Most Streptococcus are _______ ________.
Definition
facultative anaerobes
Term
Serological classification of Streptococcus is useful in _______.
Definition
identification
Term
The Lancefield classification Developed by Rebecca Lancefield in 1938 was
Based on ______ ______.
Definition
serological classification
Term
The Lancefield classification puts various strains of Streptococcus into _____ different groups.
Definition
19.
Term
Lancefield groups go from _____ to ____.
Definition
A – S
Term
Lancefield groups A and B are ______ and ______.
Definition
GAS and GBS
Term
GAS and GBS Include the significant streptococcal pathogens of _____.
Definition
humans
Term
There are _____ species of Streptococci
only a few (5) are ______ _______.
Definition
Thirty, human pathogens
Term
Group A Streptococcus =
Definition
Streptococcus pyogenes
Term
What do Group A (GAS) Streptococcus pyogenes look like?
Definition
Looks like chain of purple pearls on slide
Term
Group A Streptococcus: GAS
On blood agar plate, Streptococcus pyogenes forms ______ colonies surrounded by _____ of beta-hemolysis on blood agar plates.
Definition
white, zone
Term
Streptococcus pyogenes lyse _____ leaving a ____ zone around ____ colony.
Definition
RBC, clear, white
Term
What is the main representative of group A Streptococcus (GAS)?
Definition
Streptococcus pyogenes
Term
What is the most serious streptococcal pathogen?
Definition
Streptococcus pyogenes
Term
Streptococcus pyogenes has a number of
Structures, Enzymes and Toxins that enable it to survive as a ______.
Definition
pathogen
Term
Pathogenic strains of Streptococcus pyogenes form _____
Definition
capsules
Term
What are the structural components and enzymes of Streptococcus pyogenes? 5 things?
Definition
1.Protein M (protein A in S. aureus)
2.Hyaluronic acid capsule
3.Streptokinase Enzymes
4.Hyaluronidase = spreading factor
Term
Protein M ________ complement. Thereby interfering with opsonization, phagocytosis, and lysis of bacteria.
Definition
destabilizes,
Term
Hyaluronic acid capsule acts to ______ the bacteria
Definition
camouflage(WBC cannot recognize)
Term
Streptokinase Enzymes ______ blood clots and _______ spread of Streptococcus pyogenes into ______ and _____tissue
Definition
dissolves, facilitates, damaged and infected
Term
Hyaluronidase called the ______ ______, dissolves hyaluronic acid and facilitates spread of Streptococcus pyogenes into deeper tissue
Definition
spreading factor
Term
What are Streptococcus pyogenes major Extracellular Toxins? 3 things
Definition
Exotoxins
Pyrogenic toxins/ Erythrogenic toxins
Streptolysins toxins
Term
Streptococcus pyogenes :Pyrogenic toxins Stimulate______ and ______ cells to release cytokines/pyrogenes
Definition
macrophages and helper T cells
Term
Streptococcus pyogenes :Pyrogenic toxins Stimulate ______ , causes ____ , and _____ toxic shock
Definition
fever,rash and streptococcal
Term
Because Pyrogenic toxins cause blood capillaries near surface of skin to dilate producing a red rash known as ____ _____, Some scientist call the toxins _________ toxins
Definition
(scarlet fever), Erythrogenic
Term
Streptolysins toxins Cause _____ of sheep blood agar. Clear zone around colony
Definition
hemolysis
Term
Streptolysins toxins Lyses _____, _____ and _______Which interfere with O2 carrying capacity, Immunity, Blood clotting.
Definition
RBCs, WBCs and Platelets.
Term
Streptolysins toxins also lyse other tissue cells such as
Definition
Liver cells
Cardiac muscle cells
Kidney cells
Term
Humans are only significant reservoir of ___ to ____ % of population are carriers of virulent strains of Streptococcus pyogenes
Definition
5-15%
Term
Streptococcus pyogenes
Typically causes disease when:
Definition
Competing normal flora is depleted
Immunity is impaired
When large inoculum enables it to get a foot hold (infectious dose) before antibodies form against it
Term
Streptococcus pyogenes
Causes following diseases: KNOW these 7
Definition
Strep throat
Erysipelas
Streptococcal toxic shock syndrome
Necrotizing fasciitis
Scarlet fever
Rheumatic fever
Post streptococcal glomerulonephritis
Term
What is the Streptococcus pyogenes strain for Sore throat, strep throat, or tonsillitis ?
Definition
Streptococcal pharyngitis
Term
Bacteriological or serological testare needed for a sure diagnosis of ______ _____
Definition
Streptococcal pharyngitis
Term
What is Streptococcal pharyngitis treated with?
Definition
penicillin
Term
What is a Sequela?
Definition
a condition following and resulting from a disease
Term
_____ is a morbid complication that follows a disease
Definition
Sequela
Term
Some diseases leave sequela In the form of long-term or permanent
Definition
Damage to tissues or organs
Term
Examples of Sequelas:
Meningitis can result in _____
Definition
deafness
Term
Examples of Sequelas:
Strep throat can result in _____ _____
Definition
scarlet fever
Term
Examples of Sequelas:
Strep throat can result in _____ ______ disease
Definition
rheumatic heart
Term
What is Pyoderma?
Definition
streptococcal impetigo
Term
streptococcal impetigo is a localized _____ ______.
Definition
skin disease
Term
streptococcal impetigo Begins as small _____producing lesions that itch, break open and form contagious yellow crust on skin
Definition
pus
Term
Pyoderma = ____ producing lesion on skin of face, arms, and legs
Definition
pus
Term
Erisipelas =Slightly more invasive form of ______ infection spreads to _____ and _____ tissues.
Definition
skin, dermis and subcutaneous
Term
Streptococcus pyogenes: Erisipelas
is called Erysipelas when this infection involved ____ _____.
Triggers pain and inflammation
Definition
lymph nodes
Term
Hogs can get ______ and infected hogs can infect______
Definition
Erysipelas, humans
Term
Streptococcus pyogenes can also cause
Streptococcal _____ _____ ______.
Definition
toxic shock syndrome
Term
Patients are bacteremic with severe multi-system infections and failure if they contract ______?
Definition
TSS
Term
Necrotizing fasciitis =
Definition
Flesh eating strep
Term
Necrotizing fasciitis Life threatening with mortality rate of ____% if left untreated
Definition
70%
Term
Flesh eating Streptococcus pyogenes
follows the path of _____ between subcutaneous tissue and muscle and CT surrounding the muscles
Definition
fascia
Term
Flesh eating Strep Streptococcus pyogenes first symptoms
Definition
Swelling, heat, redness
Skin changes from red to purple to blue to large blisters
Term
Flesh eating Streptococcus pyogenes
Skin dies and muscle becomes infected. Need rapid therapy of which drug?
Definition
penicillin G
Term
What are used to remove the dead tissue sometimes?
Definition
maggots
Term
Rheumatic Fever is a sequila. which disease does it follow? What does it result in?
Definition
strep throat
Results in damage of heart valves and heart muscle.
Term
Rheumatic Fever is an _______ response to Streptococcus pyogenes.
Definition
Autoimmune
Term
Rheumatic Fever is ______ mediated. Antigens in heart are similar to
Antigens on Streptococcus pyogenes, and our_____attach to heart muscle, attack it and causes ____which can damage the heart valves.
Definition
antibody, antibodies, myocarditis
Term
Acute Glomerulonephritis = AGN
Antibody-antigen complexes of ____ ____. Accumulate in ______.
Obstructs blood flow through _____.
Definition
Streptococcus pyogenes, glomeruli, kidneys
Term
Acute Glomerulonephritis = AGN
Sequela of ______ ______.
Definition
Streptococcus pyogenes
Term
Acute Glomerulonephritis = AGN
Leads to ______ and ______ urine output
Face may be puffy, urine is _____ colored
Definition
hypertension, low, tea
Term
Acute Glomerulonephritis = AGN may cause _____kidney damage in adults.
Definition
Irreversible
Term
Diagnosis of Streptococcus pyogenes
Definition
Observation of Gram-positive bacteria in short chains or pairs
Term
Prevention of Streptococcus pyogenes:
Antibodies against M protein provide long-term protection against future infection of S. pyogenes ONLY it it is _____?
Definition
the same strain of Streptococcus
(can get strep throat many times from different strains)
Term
GBS = Group B Streptococcus:
Definition
Streptococcus agalactiae
Term
Not until Lancefield classification scheme was Streptococcus agalactiae(GBS)Recognized in _____
Definition
humans
Term
Streptococcus agalactiae
Newborns with no antibodies are at risk of infection if born to previously uninfected mothers with no antibodies. Why is this?
Definition
No antibodies for S. agalactiae were passed to fetus during late gestation
Term
Streptococcus agalactiae normally colonizes which 3 things?
Definition
GI, Genitals, and urinary tract
Term
Newborns can pick up _____ ______ in birth canal during birth
Definition
S. agalactiae
Term
CDC recommends prophylactic administration of ______ at birth to neonates whose mother’s urinary/genital tract is colonized with S. agalactiae
Definition
penicillin
Term
Streptococcus _____Causes serious infections in newborns and immunocompromised people
Definition
agalactiae
Term
think Baby for GBS-
Streptococcus _____ causes Causes of neonatal pneumonia, sepsis, and meningitis
Definition
agalactiae
Term
Streptococcus agalactiae resides in human ____, ____, _____, and ______.
Definition
pharynx, vagina, urinary tract, and large intestine.
Term
Viridans Streptococci
(verdant, viridis L. = green)
Definition
Many produce green pigment when grown on blood agar
Term
Viridans Streptococci is the normal flora in ____, ____, _____, ____ and _____.
Definition
Oral cavity, pharynx, GI tract, Urinary and genital tracts
Term
Ex. Of Viridans Streptococci
Streptococcus mutans causes _____ _____.
Definition
dental caries.
Term
During a dental procedures (routine cleaning teeth) there may be showers of Streptococcus mutans into the ______
Definition
bloodstream
Term
Streptococcus mutans may cause
Definition
endocarditis.
Term
People with heart conditions or heart murmurs are given _____ antibiotics before visit to dentist.
Definition
Prophylactic
Term
Streptococcus pneumoniae Gram ____ cocci
Definition
positive
Term
Streptococcus pneumoniae most commonly forms pairs called ______, formally called _______ _______
Definition
diplococci
Diplococcus pneumoniae
Term
There are ____ strains of Streptococcus pneumoniae discovered by ______. There are vaccines against ____ strains.
Definition
92,Louis Pasteur, 23
Term
_____ % of all people carry Streptococcus pneumoniae as normal flora in mouth and pharynx without causing harm BUT if it moves to lungs causes
Definition
75%, pneumonia
Term
Steptococcus pneumoniae most common cause of which 3 things?
Definition
Pneumonococcal pneumonia
Sinusitis
Otitis media
Term
Streptococcus pneumoniae important cause of _______ ________ (since we now have a vaccine for Hemophilus influenzae, HiB vaccine), _____ and _____.
Definition
Pneumococcal meningitis
Bacteremia/sepsis
Endocarditis
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