Term
| mRNA circularization occurs as the result of interaction between which two proteins? |
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Definition
| cap-binding protein and poly-A tail binding protein |
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Term
| mRNA circularize facilitates ____ recycling and makes the process of __________ more efficient. |
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Definition
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Term
| What are three modifications of the histone tail? |
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Definition
| acetylation, methylation, ubiquitination |
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Term
| When the DNA in the nucleosome is treated with DNase I and then run on a gel, there are 10 base pair gaps that appear. Why do these gaps appear? |
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Definition
| These are the regions of DNA where the minor groove is facing the nucleosome |
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Term
| How many times does the DNA wrap around a nucleosome? |
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Definition
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Term
| The basic repeating structural unit of chromatin is the _nucleosome_, which contains ____ proteins, each present at _____ copies. |
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Definition
| 4 proteins: H2A, H2B, H3, H4 each present at 2 copies |
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Term
| How many base pairs are associated with a nucleosome? |
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Definition
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Term
| The ____ enzyme can be used to determine the rotational phasing of the DNA on a nucleosome, whereas the __________ enzyme is frequently used to determine the translational phasing. |
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Definition
| DNAse I; Micrococcal nuclease |
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Term
| Where does the acetyl group for histone acetylation come from? |
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Definition
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Term
| Where does the methyl group for histone methylation come from? |
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Definition
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Term
What is the correct order of the steps in mapping the nucleosome?
a. Expose briefly to DNAseI/MN
b. Run on southern blot
c. Isolate DNA and cut with restriction enzyme
d. Isolate nuclei |
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Definition
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Term
| What was the first HAT to be identified/ cloned and what organisms ? |
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Definition
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Term
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Definition
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Term
| CpG islands are usually found near what? |
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Definition
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Term
| What is the full name of SILAC? |
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Definition
| Stable isotope labelling with amino acids in cell culture |
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Term
| In the procedure SILAC, two pools of proteins are distinguished by differences in _________ due to the incorporation of ___________. |
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Definition
| mass, heavy isotopes of carbon in amino acids |
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Term
| Class II HDACs can shuttle between: |
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Definition
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Term
| which of these are class I HDACS? 1, 2, 3, 4, 5, 6, 7, 8 |
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Definition
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Term
| Methylation at lysine ____ of histone H3 is associated with gene silencing, whereas methylation at lysine ___ is associated with gene expression. |
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Definition
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Term
| The ____ mutants do not allow haploid yeast to switch mating types. Mutations in the ____ genes can suppress these mating switch mutations. |
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Definition
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Term
| True or False. SWI mutations are specific for mating type switching and have no other defects. |
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Definition
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Term
| What domain is found in many proteins that are histone methyl transferases? |
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Definition
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Term
| SWI mutants cannot switch their mating type because they can't activate expression of ____? |
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Definition
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Term
| MLL/SET I acetylates histone H3 at lysine ___ and associates with __ |
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Definition
| H3K4, Elongating RNA polymerase II complex. |
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