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NEU Microbiology Spring 2011 Lec 9
These flashcards cover all review material from the book at the end of the chapter and from the review question slide for lecture 9
23
Microbiology
Undergraduate 1
02/15/2011

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Cards

Term
What is the size of the E. coli genome?
Definition

The E. Coli genome is about 5MB long

Term
What is the average size of an E. coli protein?
Definition

The average E. coli protein consists of about 300 amino acids

Term
What do you call a pladmis that can integrate into the chromosome?
Definition

A plasmid that can integrate itself into the chromosome is an episome

Term
What is a selectable mutation?
Definition

A selectable mutation is any sort of mutation which you can artificially affect the further division of parental colonies

Term
What is a nonsense mutation?
Definition

A nonsense mutation is a mutation that results in a stop codon where a normal codon should be

Term
What is a true revertant?
Definition

A true revertant is an organism whose original mutation was actually mutated completely back by chance. In "true" revertants the first mutation is completely undone by the second and the cell (and any resulting colonies on a plate) will result in normal phenotypes provided there are no other mutations

Term
What is the LexA protein?
Definition

The LexA protein represses SOS genes needed for DNA repair for things like DNA polymerases

Term
What is homologous recombination?
Definition

Homologous recombination happens when recombination results in the transfer of homologous genes

Term
What are competent cells?
Definition

Competent cells are cells that can take up DNA and transform themselves according to that new DNA

Term
What is the difference between generalized and specialized transduction?
Definition

In generalized tranduction, any old gene can be involved whereas in specialized there is a very specific kind of gene transfer that takes place

Term
What is an auxotroph?
Definition

An auxotroph is an organism that has developed a nutritional requirement, often as a result of mutation

Term
What is a cistron?
Definition

A cistron is a gene as defined by the cis-trans test; a segment of DNA (or RNA in some cases) that encodes a single polypeptide chain

Term
What is a heteroduplex?
Definition

A heteroduplex is a DNA double helix composed of single strands rom two different DNA molecules

Term
What is an hfr cell?
Definition

An hfr cell is a cell with the F plasmid integrated into the chromosome

Term
What is an induced mutation?
Definition

An induced mutation is a mutation caused by external agents such as mutagenic chemicals or radiation

Term
What is an insertion sequence (IS)?
Definition

An insertion sequence is the simplest type of transposable element which carries only genes involved in transposition

Term
What is a mutator strain?
Definition

A mutator strain is a mutant strain in which the rate of mutation is increased

Term
What is rolling circle replication?
Definition

Rolling circle replication is a mechanism of replicating double-stranded circular DNA that starts by nicking and unrolling one strand and using the other (still circular) strand as a template for DNA synthesis

Term
What is screening?
Definition

Screening is a procedure that permits the identification of organisms by phenotype or genotype but does not inhibit or enhance the growth of particular phenotypes or genotypes

Term
What is a transition mutation?
Definition

A transition mutation is one in which a purine is replaced with another purine (guanine to adenine or vice versa) or a pyrimidine is replaced with another pyrimidine (thymine to cytosine or vice versa)

Term
What is a transposable element?
Definition

A transposable element is a genetic element with the ability to move (transpose) from one site to another

Term
What is a transversion mutation?
Definition

A transversion mutation occurs when a purine replaces a pyrimidine or vice versa

Term
What is a wildtype strain?
Definition

A wildtype strain is a bacterial strain that is isolated from nature

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