Term
What is the size of the E. coli genome? |
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Definition
The E. Coli genome is about 5MB long |
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Term
What is the average size of an E. coli protein? |
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Definition
The average E. coli protein consists of about 300 amino acids |
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Term
What do you call a pladmis that can integrate into the chromosome? |
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Definition
A plasmid that can integrate itself into the chromosome is an episome |
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Term
What is a selectable mutation? |
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Definition
A selectable mutation is any sort of mutation which you can artificially affect the further division of parental colonies |
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Term
What is a nonsense mutation? |
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Definition
A nonsense mutation is a mutation that results in a stop codon where a normal codon should be |
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Term
What is a true revertant? |
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Definition
A true revertant is an organism whose original mutation was actually mutated completely back by chance. In "true" revertants the first mutation is completely undone by the second and the cell (and any resulting colonies on a plate) will result in normal phenotypes provided there are no other mutations |
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Term
What is the LexA protein? |
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Definition
The LexA protein represses SOS genes needed for DNA repair for things like DNA polymerases |
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Term
What is homologous recombination? |
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Definition
Homologous recombination happens when recombination results in the transfer of homologous genes |
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Term
What are competent cells? |
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Definition
Competent cells are cells that can take up DNA and transform themselves according to that new DNA |
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Term
What is the difference between generalized and specialized transduction? |
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Definition
In generalized tranduction, any old gene can be involved whereas in specialized there is a very specific kind of gene transfer that takes place |
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Term
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Definition
An auxotroph is an organism that has developed a nutritional requirement, often as a result of mutation |
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Term
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Definition
A cistron is a gene as defined by the cis-trans test; a segment of DNA (or RNA in some cases) that encodes a single polypeptide chain |
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Term
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Definition
A heteroduplex is a DNA double helix composed of single strands rom two different DNA molecules |
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Term
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Definition
An hfr cell is a cell with the F plasmid integrated into the chromosome |
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Term
What is an induced mutation? |
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Definition
An induced mutation is a mutation caused by external agents such as mutagenic chemicals or radiation |
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Term
What is an insertion sequence (IS)? |
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Definition
An insertion sequence is the simplest type of transposable element which carries only genes involved in transposition |
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Term
What is a mutator strain? |
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Definition
A mutator strain is a mutant strain in which the rate of mutation is increased |
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Term
What is rolling circle replication? |
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Definition
Rolling circle replication is a mechanism of replicating double-stranded circular DNA that starts by nicking and unrolling one strand and using the other (still circular) strand as a template for DNA synthesis |
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Term
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Definition
Screening is a procedure that permits the identification of organisms by phenotype or genotype but does not inhibit or enhance the growth of particular phenotypes or genotypes |
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Term
What is a transition mutation? |
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Definition
A transition mutation is one in which a purine is replaced with another purine (guanine to adenine or vice versa) or a pyrimidine is replaced with another pyrimidine (thymine to cytosine or vice versa) |
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Term
What is a transposable element? |
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Definition
A transposable element is a genetic element with the ability to move (transpose) from one site to another |
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Term
What is a transversion mutation? |
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Definition
A transversion mutation occurs when a purine replaces a pyrimidine or vice versa |
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Term
What is a wildtype strain? |
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Definition
A wildtype strain is a bacterial strain that is isolated from nature |
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