Term
Which steps in the central dogma of DNA are only found in virus? |
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Definition
RNA can be made into more RNA and RNA can be made into DNA using reverse transcriptase |
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Term
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Definition
Supercoiled DNA is DNA that is compressed to save space within the cell. Negatively supercoiled DNA is DNA which is somewhat unwound whereas positively supercoiled DNA is overwound |
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Term
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Definition
Relaxed DNA is a segment of DNA that has the amount of turns in it that is predicted by the number of base pairs in the sequence |
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Term
What is the difference between chromosomes and plasmids? |
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Definition
Chromosomes and plasmids can both encode relevent genetic information but plasmids are typically smaller and encode for things like resistance to various environmental factors like antibiotics |
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Term
What does DNA helicase do? |
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Definition
DNA helicase unwinds the DNA and enables better access for things like replication |
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Term
Why is there a need for an RNA primer during replication? |
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Definition
RNA primers cap the lagging strans present on the 3'-5' strand. Replication lags somewhat and Okazaki fragments are left behind which need to be capped. DNA polymerase and then DNA ligase then come in and fix these gaps |
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Term
What part of the RNA polymerase recognizes the promoter? |
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Definition
The sigma factor of RNA polymerase recognizes the promoter |
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Term
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Definition
The pribnow box is the area located about 10 base pairs away from the start of translation |
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Term
What is the reading frame? |
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Definition
The reading frame means that a triplet codon is needed for translation to begin at the correct site |
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Term
What is aminoacyl-tRNA synthase? |
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Definition
Aminoacyl-tRNA synthase is an enzyme that catalyzes attachment of an amino acid to its cognate tRNA |
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Term
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Definition
An anticodon is a sequence of three bases in a tRNA molecule that base pairs with a codon during protein synthesis |
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Term
What is chaperonin (or simply a molecular chaperone)? |
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Definition
Chaperonin is a protein that helps other proteins fold or refold from a partly denatured state |
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Term
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Definition
Codon bias is the nonrandom usage of multiple codons encoding the same amino acid |
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Term
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Definition
DNA gyrase is an enzyme found in most prokaryotes that introduces negative supercoils in DNA |
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Term
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Definition
DNA polymerase is an enzyme that synthesizes a new strand of DNA in the 5'-3' direction using an antiparallel DNA strand as a template |
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Term
What is a genetic element? |
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Definition
A genetic element is a structure that carries genetic information such as a chromosome, a plasmid or a viral genome |
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Term
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Definition
A genome is the total complement of genes contained in a cell or virus |
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Term
What is an informational macromolecule? |
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Definition
An informational macromolecule is any large polmeric molecule that carries or comes from genetic information such as DNA, RNA and proteins |
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Term
What is a lagging strand? |
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Definition
A lagging strand is the new strand of DNA that is synthesized in short pieces during DNA replciation and then joined together later |
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Term
What is the leading strand? |
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Definition
The leading strand is the strand of DNA that is synthesized continuously during DNA replication |
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Term
What is a nonsense codon? |
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Definition
A nonsense codon is just another name for a stop codon. A nonsense mutation is a mutation where a stop codon is put in place of another codon but a nonsense codon simply refers to any of the 3 stop codons |
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Term
What is an open reading frame (ORF)? |
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Definition
And open reading frame is a sequence of DNA or RNA that could be translated to give a polypeptide |
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Term
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Definition
An operon is a cluster of genes that are co-transcribed to give a single messenger RNA |
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Term
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Definition
A primer is an oligonucleotide to which DNA polymerase can attach the first deoxyribonucleotide during DNA replication |
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Term
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Definition
A promoter is a site on DNA to which RNA polymerase binds to commence transcription |
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Term
What is a replication fork? |
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Definition
A replication fork is the site on the chromosome where DNA replication occurs and where the enzymes replicating the DNA are bound to untwisted ssDNA |
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Term
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Definition
RNA polymerase is an enzyme that synthesizes RNA in the 5'-3' direction using a complementary and antiparallel DNA strand as a template |
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Term
What is semiconservative replication? |
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Definition
Semiconservative replication is DNA which yields new double helicases each consisting of one parental and one progeny strand of DNA |
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Term
What is a signal sequence? |
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Definition
A signal sequence is a special N-terminal sequence of approximately 20 amino acids that signals that a protein should be exported across the cytoplasmic membrane |
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Term
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Definition
Wobble is a less rigid form of base pairing allowed only in codon-anticodon pairing |
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