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NEU Microbiology Spring 2011 Lec 4
These flashcards cover all review material at the end of lecture 4
37
Microbiology
Undergraduate 1
01/27/2011

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Term
How does movement occur in peritrichously flagellated prokaryotes?
Definition

Bundled flagella unbundle and then "tumble" to reorient to the desired direction. Once they're in place, the flagella will once again begin to rotate and keep the cell moving

Term
What is the name of the filament protein of flagella?
Definition

The filement protein in flagella is simply flagellin

Term
How many rings does a flagella of a Gram+ bacteria have?
Definition
There are a series of 4 rings which all help to control cell motility
Term
What are catabolic reactions?
Definition

Catabolic reactions are reactions that release energy. Examples include burning wood and consuming polysaccharides to produce energy

Term
What is the name of iron chelating molecules produced by bacteria?
Definition

The iron chelating agents are known as siderophores; they're used to obtain iron from mineral iron sources

Term
What is reduction potential?
Definition

The reduction potential is the tendency of a compound to donate electrons. The lower the reduction potential, the more likely the substance will be the reduction agent and donate electrons

Term
What is activation energy?
Definition

Activation energy is the energy required to bring the substrate of an enzyme to the reactive state

Term
What is adenosine triphosphate (ATP)?
Definition

ATP is a nucleotide that is the primary form in which chemical energy is conserved and utilized in cells

Term
What is an allosteric enzyme?
Definition

An allosteric enzyme is an enzyme containing an active site plus an allosteric site for binding an effector molecule

Term
What is an anabolic reaction?
Definition

An anabolic reaction is the sum total of all biosynthetic reactions in the cell

Term
What is anaerobic respiration?
Definition

Anaerobic respiration is a form of respiration in which oxygen is absent and alternative electron acceptors are reduced

Term
What is the aseptic technique?
Definition

The aseptic technique are the manipulations to prevent contaminations of sterile objects or microbial cultures during handling

Term
What is ATPase (ATP synthase)?
Definition

ATPase (ATP synthase) is a multiprotein enzyme complex embedded in the cytoplasmic membrane that catalyzes the synthesis of ATP coupled to dissipation of the proton motive force

Term
What are catabolic reactions?
Definition

Catabolic reactions are biochemical reactions that lead to enrgy conservation (usually as ATP) by the cell

Term
What are chemolithotrophs?
Definition

Chemolithotrophs are organisms that can grow with inorganic compounds as electron donors in energy metabolism

Term
What is the citric acid cycle?
Definition

The citric acid cycle is a cyclical series of reactions resulting in the conversion of acetate into two molecules of carbon dioxide along with some other products (including 2 ATP!)

Term
What is a coenzyme?
Definition

A coenzyme is a small and loosely bound nonprotein moelcule that participates in a reaction as part of an enzyme

Term
What is a complex medium?
Definition

A complex medium is a culture medium composed of digests of chemically unidentified substances such as yeast and meat extracts

Term
What is a culture medium?
Definition

A culture medium is an aqueous solution of various nutrients suitable for the growth of microorganisms

Term
What is a defined medium?
Definition

A defined medium is a medium in which the precise chemical composition is already known

Term
What is an electron acceptor?
Definition

An electron acceptor is a substance that can accept electrons from an electron donor; the acceptor then becomes reduced in the process

Term
What is an electron donor?
Definition

An electron donor is a substance that can donate electrons to an electron acceptor; the donor then becomes oxidized through the process

Term
If an reaction is endergonic, what does that mean about the reaction?
Definition

The reaction requires energy to take place. Without the input of energy, the reaction wouldn't take place

Term
When a reaction is said to be exergonic, what does that mean?
Definition

It means that the reaction releases energy. A good example would be gasoline combining with air to fuel various things like cars and trucks

Term
What is feedback inhibition?
Definition

Feedback inhibition is a process in which an excess of the end product of a multistep pathway inhibits activity of the first enzyme in the pathway

Term
What is fermentation?
Definition

Fermentation is an anaerobic catabolism in which an organic compound is both an electron donor and an electron acceptor and ATP is produced by substrate-level phosphorylation

Term
What is free energy (G)?
Definition

Free energy is the energy available to do work; G0' is the free energy under standard conditions

Term
What is glycolysis?
Definition

Glycolysis is a biochemical pathway in which glucose is fermented yielding ATP and various fermentations products; also called the Embden-Mayerhof pathway

Term
What is metabolism?
Definition

Metabolism is the sum total of all the chemical reactions that occur in a cell

Term
What is oxidative phosphorylation?
Definition

Oxidative phosphorylation is the production of ATP from a proton motive force formed by electron transport of electrons from organic or inorganic electron donors

Term
What is photophosphorylation?
Definition

Photophosphorylation is the production of ATP from a proton motive force formed from light-driven electron transport

Term
What is the proton motive force?
Definition

The proton motive force is a source of potential energy resulting from the separation of charge and protons from hydroxyl ions across the cytoplasmic membrane

Term
What is a pure culture?
Definition

A pure culture is a culture that contains a single kind of microorganism

Term
What is respiration?
Definition

Respiration is the process in which a compound is oxidized with oxygen gas as the terminal electron acceptor, usually accompanied by ATP production by oxidative phosphorylation

Term
What is a siderophore?
Definition

A siderophore is an iron chelator that can bind iron present at very low concentrations

Term
What is substrate-level phosphorylation?
Definition

Substrate-leve phosphorylation is the production of ATP by the direct transfer of an energy-rich phosphate molecule from a phosphorylated organic compound to ADP

Term
What is the definition of sterile?
Definition

When something is sterile that means that it is absent of all microorganisms (including viruses)

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