Term
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Definition
Gram neg diplococci. Inhabit the mucous membranes of warm-blooded hosts. non-motile and non-spore forming. Grows in CO2 |
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Term
Neisseriaceae are catalse what? Oxidase what? |
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Definition
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Term
Neisseria gonorrhoeae causes what in males? |
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Definition
Urethritis Dyuria Urethral discharge |
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Term
Neisseria gonorrhoeae causes what in females? |
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Definition
Endocervix infection, symptoms include: cervicovaginal discharge, lower ab pain, abnormal periods |
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Term
What are some populations with a high prevalence of neisseria gonorrhoeae? |
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Definition
Low socioeconimic class Residence in urban areas Drug abuse Prostitute |
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Term
Lab identification of Neisseria gonorrhoeae? |
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Definition
Grows in translucent, raised, moist, grayish-white colinies on CHOC or MTN agar. Produces bubbles to superoxol Can be identified usingMolecular probe technology |
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Term
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Definition
Selective media for Neisseria Contains: Vancomycin- Inhibits gram pos Colistin- Inhibits gram neg Nystatin- Inhibits yeasts Trimethylprym- Inhibits flagellar action of proteus. (Prevents from swarming) |
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Term
Neisseria gonorrhoeae carbohydrate test and enzyme test |
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Definition
Carb test- Only glucose pos Enzyme test- Hydroxypropyl aminopeptidase positive |
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Term
What is the standard for diagnosis of Neisseria gonorrhoeae? |
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Definition
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Term
Neisseria meningiditis symptoms: |
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Definition
Confusion, headache, fever. Causes petechiae which are small hemorrhagic skin lesions |
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Term
Neisseria meningiditis acute form |
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Definition
Slight fever, respiratory tract symptoms |
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Term
Neisseria meningiditis chronic form |
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Definition
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Term
Spread of Neisseria meningiditis |
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Definition
Spread by respiratory droplets or direct contact with respiratory secretions. Humans are the only host. |
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Term
Lab identification of Neisseria meningiditis |
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Definition
The presence of inflammatory cells has prognostic value (with fulminant, rapidly fatal disease, many organisms are seem with few inflammatory cells) Colony on CHOC agar and MTM agar is similar to N. gonorrhoeae. Will grow on blood agar. |
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Term
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Definition
Test used to identify Neisseria meningiditis. Performed to identify meningococcal capsular polysaccharides. Pos test is helpful for early diagnosis. Neg test does not rule out meningitis caused by other organisms. |
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Term
Neisseria meningiditis carbohydrate and enzyme test results |
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Definition
Carb- Glucose and maltose positive Enzyme- Gamma glutamylaminopeptidase positive |
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Term
Neisseria species are normal flora of the |
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Definition
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Term
N. subflava, N. mucosa, N. sicca and N. flavescens may cause what? |
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Definition
Endocarditis in patients with prosthetic heart valves or IVDA |
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Term
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Definition
Can grow on selective media. Isolated from genital sites, CSF and blood |
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Term
Lab results for other Neisseria spp. |
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Definition
Carb test- Glucose, lactose and maltose pos Enzyme test- beta-D-galactosidase ONPG-hydrolyzed |
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Term
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Definition
Recovered from patients with AIDS as a cause of nosocomial pneumonia. Grows on BAP and CHOC |
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Term
N. cinera carb test and enzyme test |
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Definition
Carb- No production of acid from carbs in the CTA-based or rapid carb tests. Weakly pos glucose has been reported for some automated systems. Enzyme test- Pos for hydroxypropyl aminopeptidase |
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Term
Colistin disk susceptibility test |
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Definition
Can differentiate N. cinera from N. gonorrhoeae. N cinera= S. N gonorrhoae= R |
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Term
Only rod-shaped member of the genus Neisseria? |
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Definition
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Term
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Definition
May cause lower respiratory tract infection in older people or those with chronic disease. Also causes otitis media and acute sinitis in children |
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Term
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Definition
Acquired through the acquisition of Moraxella catarrhalis at birth from the mother's colonized genital tract. |
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Term
Lab identification of Moraxella catarrhalis |
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Definition
Grows on BAP and CHOC. Slides on agar. Carb test: assacharolytic Enzyme test: Neg for all enzymes tested. DNase: positive Chromogenic cephalosporin test: posive (Production of an inducible, cell-associated beta-lactamase) |
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Term
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Definition
Quick spot test used to identify Moraxella catarrhalis. The organism hydrolyzes the ester-linked butyrate in disk to produce a color change. |
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Term
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Definition
The minimum amount of antibiotics that can be used to inhibit the spread of an organism. |
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Term
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Definition
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Term
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Definition
Prevents organism from growing. |
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Term
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Definition
Set up serial dilution with antimicrobial agent to determine which of the concentrations is the least concentration which prevents the organism from growing. To find the correct tube. Find the first tube in which the organism appears not to be growing |
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Term
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Definition
The minimum concentration of anitbiotic that will kill a specific microorganism. |
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Term
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Definition
After performing a serial dilution with a specific organism and antibioticm, quantitatively subculture all clear wells to determine what the minimum concentration of antibiotic is that will kill the organism |
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Term
Which is larger: The bacteriocidal concentration or the bacteriostatic concentration? |
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Definition
The bacteriocidal concentration |
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Term
Is it possible to perform an MBC on a cidal agent? |
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Definition
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Term
Is it possible to perform an MBC on a static agent? |
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Definition
No because the agent will only prevent the organism from growing, not kill it |
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Term
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Definition
Involves using patient serum which is tested against a certain bacteria to see if it will kill it. |
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Term
What are some things that are important to standardize in antimicrobial testing? |
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Definition
Conc of agent Conc of organism Standardized McFarland inoculation Size of disk Plate thickness How far apart discs are Incubation time Standardized zone of clearing Incubation temp Manufacture consistency |
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Term
Kirby-Bauer Susceptibility Test |
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Definition
A method to determine the sensitivity of microorganisms to specific antimicrobial drugs; greater drug efficacy yields larger microbe-free zones surrounding drug-containing disks after overnight growth on solid media. (MIC Test) |
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Term
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Definition
Quantitative technique for determining the antimicrobial susceptibility of microorganisms (MIC Test) |
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Term
How can you get a falsely resistant MIC? |
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Definition
Not enough antibiotic Too much organism |
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Term
How can you get a falsely susceptible MIC? |
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Definition
Too few organisms in tubes Too much antibiotics in tubes Dilution is off |
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Term
Set up a normal MIC using serial dilution |
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Definition
1. Make serial dilution by dividing each preceeding tube concentration by 2. (Take tube 1 conc and divide it in half and repeat) 2. Add TSB or Mueller-Hinton broth to each test tube 3. Add necessary amount of antibiotic to the first tube. Transfer the same amount of solution into the next tube and repeat for all tubes. 4. Need a growth control (no antibiotic) 5. Need a sterility control (no organism) |
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Term
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Definition
1. Inoculate a plate for each clear tube that was present. Streak to obtain a colony count. 2. Incubate plates 3. Examine the plates for presence of growth of test organism. |
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