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NBDE Flash! - Biochemistry
NBDE Flash! Part 1 Review Series. Home Page: http://www.flashcardmachine.com/p/nbdeflash
357
Dentistry
Professional
02/10/2012

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Term
What is the type of mutation that changes the reading frame?
Definition
Frameshift
Term
What is the function of a gap junction?
Definition
Allows adjacent cells to communicate for electric and metabolic functions.
Term
What is the primary source of energy for the Liver?
Definition
Amino acids, lipids, glucose, fructose, and lactate
Term
What disease lack aldolase B causing hypoglycemia, jaundice, and cirrhosis?
Definition
Fructose intolerance
Term
What toxin inhibits lipoic acid?
Definition
Arsenic (Lipoic Acid is a cofactor for Pyruvate Dehyd.)
Term
What are the net reactants and products in the reaction that Pyruvate Dehydrogenase catalyzes?
Definition
Reactants:1. Pyruvate 2. CoA 3. NAD Products:1. Acetyl CoA 2. CO2 3. NADH
Term
Pyruvate dehydrogenase deficiency: Congenital or Acquired
Definition
Both. Acquired cases happen in cases of B1 deficiency (such as in alcoholics.)
Term
What is the rate-limiting step of the TCA?
Definition
Isocitrate to alpha-ketoglutarate by isocitrate dehydrogenase
Term
Which tissues and organs primarily convert pyruvate into lactate?
Definition
1. RBCs and WBCs 2. Lens and cornea 3. Renal medulla 4. Testes
Term
What stimulates and inhibits isocitrate dehydrogenase?
Definition
Stimulate: ADP; Inhibit: 1. ATP 2. NADH
Term
Which reagent sequentially removes C-terminal residues from a polypeptide?
Definition
Carboxypeptidase
Term
Where in the cell does the following occur: Fatty acid oxidation (beta-oxidation)
Definition
Mitochondria
Term
What activated carriers carry: 1-carbon units
Definition
1. Tetrahydrofolates (originally as formyl then methyl) 2. Biotin (as CO2) 3. S-adenosyl-methionine (as CH3)
Term
What is the mechanism of oligomycin?
Definition
ATPase inhibition
Term
Cathode: What does it attract?
Definition
Cations
Term
What kind of inheritance and mutation is the alpha-1-antitrypsin deficiency?
Definition
Autosomal recessive, single purine substitution (GAG to AAG)
Term
What is this molecule an activated carrier of?: S-adenosyl-methionine
Definition
Methyl groups
Term
What is the initial RNA transcript called?
Definition
heterogeneous nuclear RNA (hnRNA)
Term
What is the mechanism of electron transport inhibitors?
Definition
1. Directly inhibit electron transport causing: 2. Decreased protein gradient and decrease in O2 consumption, thereby: 3. Blocking ATP synthesis
Term
Where is thermogenin found?
Definition
Brown adipose tissue
Term
What stimulates and inhibits alpha-ketoglutarate dehydrogenase?
Definition
Stimulate: Nothing; Inhibit: 1. ATP 2. NADH 3. Succinyl CoA
Term
Pyruvate dehydrogenase: What inhibits it?
Definition
NADH (You produce NADH, soon there'll be more of me.), Acetyl-CoA (Enough of me, save your pyrvuate.)
Term
Where does the Cori Cycle happen?
Definition
In the liver and muscle/RBCs Liver: Pyruvate converts to glucose Muscle/RBCs: Glucose converts to Pyruvate
Term
What enzymes and cofactors are used in conversion of pyruvate to alanine?
Definition
Enzyme: Alanine Transaminase (ALT) Cofactors: None
Term
What is the rate-limiting enzyme in the Pentose phosphate pathway?
Definition
Glucose-6-Phosphate Dehydrogenase
Term
Where in the electron transport chain is O2 reduced to 2H2O?
Definition
Complex IV
Term
Pyruvate dehydrogenase deficiency: Treatment
Definition
Increased intake of ketogenic nutrients (such as high fat content or increased lysine and leucine)
Term
What stimulates and inhibits citrate synthase?
Definition
Stimulate: Nothing; Inhibit: ATP
Term
What is the enzyme involved in processing tRNA
Definition
Aminoacyl tRNA synthetase (uses 1 ATP)
Term
What is the purpose of the Cori cycle?
Definition
Transfers excess reducing equivalents from RBCs and the muscle to liver so they can function anaerobically
Term
Pyruvate dehydrogenase: What stimulates it?
Definition
excess pyruvate?
Term
Pyruvate dehydrogenase: What does it do?
Definition
Pyruvate dehydrogenase converts pyruvate to acetyl-coA, and produces NADH and CO2.
Term
Lipoamide or lipoate: Which is a cofactor for pyruvate dehydrogenase?
Definition
Lipoate (Lipoic acid)
Term
What is antimycin A?
Definition
An electron transport inhibitor.
Term
Name three classes of oxidative phosphorylation poisons.
Definition
1. Electron transport inhibitors 2. ATPase inhibitors 3. Uncoupling agents
Term
What enzymes and cofactors are used in conversion of pyruvate to oxaloacetate?
Definition
Enzyme: Pyruvate Carboxylase (contains biotin and magnesium) Cofactors: CO2 and ATP
Term
# of Aas in one turn of alpha-helix?
Definition
3.60
Term
What disease state is glycolytic enzyme deficiency generally associated with?
Definition
Hemolytic anemia
Term
What stimulates pyruvate dehydrogenase?
Definition
ADP (need more ATP. Inhibits PD kinase and stimulates PD phosphatase.)
Term
What are the reactants and products in the reaction catalyzed by pyruvate carboxylase?
Definition
Reactant: Pyruvate (with CO2 and ATP) Product: Oxaloacetate
Term
Energy malnutrition leads to what disease?
Definition
Marasmus (as opposed to Kwashiorkor from protein malnutrition)
Term
Which major metabolic reactions require Thiamine as a cofactor?
Definition
TCA: Pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, HMP shunt: Transketolase
Term
Why is FAD used to oxidize succinate?
Definition
Succinate is not powerful enough to reduce NAD.
Term
Role of ATP in protein synthesis
Definition
ATP does tRNA Activation (charging)
Term
Why is fructose metabolism faster than glucose metabolism?
Definition
Bypasses PFK, major regulatory step of glycolysis.
Term
Where is fructokinase found?
Definition
Liver (processes most dietary fructose), kidney, small intestine
Term
Amino acids that disrupt alpha-helix?
Definition
proline, many charged aas, bulky side chains
Term
Where in glycolysis and TCA does CO2 come off?
Definition
3 places: Pyruvate to Acetyl CoA, Isocitrate to alpha-ketoglutarate, and alpha-ketoglutarate to Succinyl CoA
Term
Enzyme function: DNA helicase
Definition
Separates the two strands of DNA into single strands allowing for replication to occur.
Term
What enzyme converts Galactose-1-Phosphate to Glucose-1-Phosphate?
Definition
Uridyl transferase
Term
What enzyme converts Galactose to galactose-1-phosphate?
Definition
Galactokinase
Term
Sources of Succinyl CoA?
Definition
TCA intermediate, and from odd chained fatty acids, and from propionyl coA from metabolism of branched-chain amino acids.
Term
What is the clinical presentation of essential fructosuria?
Definition
Fructose appears in the blood and urine
Term
What is the treatment for fructose intolerance?
Definition
Decreased intake of both fructose and sucrose.
Term
What does sorbitol dehydrogenase do?
Definition
Oxidizes sorbitol to fructose.
Term
Name the purines.
Definition
Adenine and Guanine
Term
Which post-translational modification involves hydroxylation?
Definition
post-translational covalent alteration
Term
Hunter's Syndrome vs Hurler's Syndrome: Mental retardation?
Definition
Both (Hunter's ranges from mild to severe)
Term
An acid with a pKa of x serves as a buffer best at x + what?
Definition
positive or negative 1 (equal amounts of charged and uncharged acid)
Term
Which base pair bond has 2 Hydrogen bonds?
Definition
Adenine to Thymine
Term
Which base pair bond has 3 Hydrogen bonds?
Definition
Guanine to Cytosine (Colby Gage is strong!!)
Term
Which tissues have cotransport of glucose?
Definition
Epithelial cells of the intestine, renal tubular cells, and choroid plexus
Term
Heparin: Where found?/Distinguishing characteristic from other GAGs
Definition
Intracellular compound (unlike other GAGs). Found in mast cells of artery walls, especially in lungs, liver, and skin
Term
Where do GLUT1 receptors predominate over other GLUT receptors?
Definition
RBCs
Term
Name the enzymes used in the oxygen-dependent respiratory burst.
Definition
1. NADPH oxidase 2. Superoxide dismutase 3. Myeloperoxidase 4. Catalase/Glutathione peroxidase 5. Glutathione reductase 6. G-6-P D
Term
Where is pyruvate carboxylase found and not found?
Definition
Found in mitochondria of liver and kidney cells, not foudn in mitochondria of muscle
Term
What does aldose reductase do?
Definition
Reduces glucose to sorbitol
Term
Where is the mutation for G6PD?
Definition
Point mutation in coding region of the G6PD gene (X-linked)
Term
What is the famed respiratory burst?
Definition
The rapid conversion of O2 to superoxide using NADPH.
Term
What is the treatment for lactose intolerance?
Definition
Avoid milk or add lactase pills to the diet
Term
Where is aldose reductase found?
Definition
Lens, retina, Schwann cells, kidney, placenta, RBCs, and gonads
Term
Chondroitin Sulfate: Use/Mechanism
Definition
Form proteoglycan aggregates. Cartilage: Bind collagen and hold fibers in a tight, strong network
Term
Kwashiorkor: Clinical presentation
Definition
Kwashiorkor results from protein-deficient MEALS. Malabsorbtion, Edema, Anemia, Liver (fatty change), Skin lesions
Term
What happens during proteasomal degradation?
Definition
Attachment of ubiquitin to defective proteins to tag them for breakdown.
Term
Where in the cell does the following occur: Urea cycle
Definition
Pathway has steps in the mitochondria and in the cytoplasm
Term
Pepsin cleaves peptides at which side of what residues?
Definition
C-terminal side of tyrosine, phenylalanine, and tryptophan (all have phenyl groups, these are the same bonds as chymotrypsin.
Term
What is a glycoside?
Definition
Carbohydrate attached to non-carbohydrate structures.
Term
What does it mean for genetic code to be degenerate?
Definition
More than one codon may code for the same amino acid.
Term
Hyaluronic Acid: Use/Mechanism
Definition
Lubricant and shock absorber
Term
What are the key enzymes of the pentose phosphate shunt and are the reactions reversible or irreversible?
Definition
1. Glucose-6-phosphate dehydrogenase (irreversible) 2. Transketolase (reversible)
Term
What does it mean for genetic code to be non-overlapping?
Definition
Read from a fixed starting point
Term
Which steps in the citric acid cycle produce CO2?
Definition
1. Isocitrate to alpha-ketoglutarate 2. alpha-ketoglutarate to succinyl coA
Term
What is this molecule an activated carrier of?: ATP
Definition
Phosphoryls
Term
How does glucagon stimulate gluconeogenesis?
Definition
Regulation of F2,6-BP and inactivation of Pyruvate Kinase via elevation of cAMP-dependent protein kinase A.
Term
What does galactokinase do?
Definition
converts Galactose to galactose-1-phosphate
Term
What is the mechanism of CO?
Definition
Electron transport inhibition
Term
Which amino acid is coded by only one codon?
Definition
Methionine
Term
Hunter's Syndrome vs Hurler's Syndrome: Enzyme deficiency
Definition
Hunter's: Iduronate sulfatase, Hurler's: alpha-L-iduronidase
Term
Trypsin cleaves peptides at which side of what residues?
Definition
C-terminal of lysine or arginine (the most basic amino acids)
Term
Where in the cell does the following occur: Hexose Monophosphate Shunt
Definition
Cytoplasm
Term
Mutations ordered by decreasing severity of damage
Definition
1. Nonsense, 2. Missense, 3. Silent
Term
What is the mechanism of 2,4-dinitrophenol?
Definition
Uncoupling agent
Term
What enzyme phosphorylates glucose with high affinity?
Definition
Hexokinase
Term
What is NADPH used for?
Definition
1. Reductive biosynthesis 2. Reduction of oxygen directly and hydrogen peroxide indirectly 3. Cytochrome P-450 mono-oxygenase system
Term
What disease results from NADPH oxidase deficiency?
Definition
Chronic Granulomatous Disease
Term
Phosphofructokinase-1: What does it do, and what stimulates and inhibits it?
Definition
PFK-1 1-phosphorylates fructose-6-phosphate to produce Fructose-1,6-Bisphosphate.
Term
Heparan Sulfate: Where found?/Distinguishing characteristic from other GAGs
Definition
Extracellular, unlike heparin. Found in basement membrane and as a ubiquitous component of cell surfaces.
Term
What is the mechanism of 2,4-dinitrophenol?
Definition
Uncoupling agent
Term
Glucokinase: Where is it found and why does it do what it does?
Definition
Found in the liver and pancreatic beta cells. Phosphorylates glucose to sequester it after a big meal.
Term
What are the two most common glycolytic enzyme deficiencies?
Definition
Pyruvate kinase (95% of cases) followed by glucose phosphate isomerase (4% of cases)
Term
Dermatan Sulfate: Where found?/Distinguishing characteristic from other GAGs
Definition
Found in skin, blood vessels, and heart valves
Term
In nucleic acids, what kind of substitution is a transversion?
Definition
TransVersion = conVersion between types (Purine for pyrimidine or vice versa)
Term
What does alpha-amanitin do?
Definition
Inhibits RNA polymerase II leading to hepatic necrosis
Term
Name the irreversible enzymes in glycolysis.
Definition
1. Hexokinase 2. Phosphofructokinase-1 3. Pyruvate kinase 4. Pyruvate dehydrogenase
Term
Which is more serious, essential fructosuria or fructose intolerance?
Definition
Fructose intolerance, because it depletes the cells of phosphate.
Term
In aerobic metabolism of glucose, which pathway produces 38 ATP?
Definition
Malate shuttle
Term
What activated carriers carry: CH3 groups
Definition
1. S-adenosyl-methionine 2. N5-methyl-THF
Term
mRNA stop codons
Definition
1. UGA (U Go Away) 2. UAA (U Are Away) 3. UAG (U Are Gone)
Term
What is the result of lack of disaccharidase activity of intestinal mucosa?
Definition
Osmotically active disaccharides suck water out of mucosa causing osmotic diarrhea.
Term
What is the presentation of arsenic toxicity?
Definition
1. Vomiting 2. Rice water stools 3. Garlic breath
Term
Enzyme function: DNA topoisomerases
Definition
Create a nick in the helix to relieve supercoils
Term
Types of RNA and their important qualities
Definition
mRNA is the largest type. rRNA is the most abundant type. tRNA is the smallest type “Massive, Rampant, Tiny”
Term
What is the treatment for classic galactosemia?
Definition
Exclude galactose and lactose from the diet.
Term
What are the two most common glycolytic enzyme deficiencies?
Definition
Pyruvate kinase (95% of cases) followed by glucose phosphate isomerase (4% of cases)
Term
This oxidation accounts for about 2/3 of the total oxygen consumption and ATP production in most animals
Definition
Oxidation of acetyl coA to CO2 and H2O.
Term
What activated carriers carry: Formyl groups
Definition
N10-formyl-THF
Term
Where in the electron transport chain is ADP converted to ATP?
Definition
Complex V aka ATP synthase aka mitochondrial ATPase
Term
What histologic change is seen in G6PD deficiency
Definition
Heinz bodies within red blood cells
Term
True/False: RNA is synthesized 5' to 3'
Definition
TRUE
Term
What does NADPH deficiency in RBCs result in?
Definition
Hemolytic anemia
Term
DNA polymerase I: Which direction does it write?
Definition
5' to 3'
Term
Which is active?: Phosphorylated or dephosphorylated pyruvate dehydrogenase
Definition
Dephosphorylated.
Term
Which amino acids are necessary for purine synthesis?
Definition
Glycine, Aspartate, Glutamine
Term
Uses of Succinyl CoA?
Definition
TCA intermediate, and biosynthesis of heme
Term
What does eukaryotic RNA polymerase II make?
Definition
mRNA
Term
Hexokinase: Where is it found and why does it do what it does?
Definition
Found in every cell's cytoplasm. Phosphorylates glucose to proceed with glycolysis.
Term
Heparin: Use/Mechanism
Definition
Anticoagulant
Term
How much ATP is produced by anaerobic glycolysis?
Definition
2 ATP per glucose
Term
Which amino acids are basic?
Definition
Arginine, Lysine and Histidine Arginine and Lysine are increased in histones which bind negatively charged DNA. Histidine has no charge at body pH.
Term
Where in the TCA does FADH2 come from my dear?
Definition
Succinate to fumarate my sweet.
Term
Pyruvate kinase: What inhibits it?
Definition
ATP (don't need more of me), Alanine (I came from pyruvate, so we don't need any more.)
Term
Which RNA polymerase makes mRNA?
Definition
eukaryotic RNA polymerase II and prokaryotic RNA polymerase
Term
Inhibitors of electron transport from Cytochrome a+a3 to Oxygen?
Definition
Cyanide, CO, and Sodium azide
Term
What is the purpose of the Cori cycle?
Definition
Transfers excess reducing equivalents from RBCs and the muscle to liver so they can function anaerobically
Term
What does 4-epimerase do?
Definition
converts between UDP-galactose and UDP-glucose
Term
Where in the TCA does NADH come from?
Definition
Pyruvate to Acetyl CoA, Isocitrate to alpha-ketoglutarate, alpha-ketoglutarate to succinyl coA, Malate to Oxaloacetate
Term
How much ATP is produced by the citric acid cycle per molecule of glucose?
Definition
24 ATP
Term
True/False: RNA polymerase proofreads.
Definition
FALSE
Term
Name three uncoupling agents
Definition
1. UCPs (such as Thermogenin) 2. 2,4-dinitrophenol 3. aspirin
Term
True/False: Protein synthesis proceeds 3' to 5'
Definition
FALSE
Term
What enzyme phosphorylates glucose with low affinity?
Definition
Glucokinase
Term
What does it mean for genetic code to be redundant?
Definition
More than one codon may code for the same amino acid.
Term
Heterochromatin or Euchromatin: Which is transcriptionally active?
Definition
Euchromatin ("eu" means true, so think "truly transcribed")
Term
What is a reducing sugar?
Definition
A monosaccharide where the anomeric carbon (Carbon 1) is free.
Term
What is the relation of polyols to sugars?
Definition
Polyols are monosaccharides where the carbonyl group is reduced to an alcohol.
Term
What are the properties of the genetic code?
Definition
Unambiguous, Degenerate/ redundant, Commaless/nonoverlapping, and Universal
Term
DNA polymerase I: Which direction does it read?
Definition
3' to 5'
Term
Which tissues (7) need glucose as fuel?
Definition
Brain, RBCs, Renal medulla, lens, cornea, testes, exercising muscle
Term
What does the mRNA initiation codon code for?
Definition
Methionine in eukaryotes. formyl-methionine in prokaryotes.
Term
What happens during post-translational covalent alterations?
Definition
Either: 1. Phosphorylation 2. Glycosylation 3. Hydroxylation
Term
Role of E site in protein synthesis
Definition
E site holds Empty tRNA as it Exits
Term
What is the etiology of fructose intolerance?
Definition
1. Lack of aldolase B 2. Build up of Fructose-1-Phosphate 3. Decrease in available phosphate 4. Inhibition of glycogenolysis and gluconeogenesis
Term
What activates isocitrate dehydrogenase?
Definition
ADP
Term
Which post-translational modification involves phosphorylation?
Definition
post-translational covalent alteration
Term
Where in the cell does the following occur: Steroid synthesis
Definition
Smooth endoplasmic reticulum in the cytoplasm
Term
Where in the cell does the following occur: Fatty acid synthesis
Definition
Cytoplasm
Term
Where does NADPH come from?
Definition
HMP shunt
Term
What vitamin is necessary for regeneration of S-adenosyl-methionine?
Definition
Vitamin B12
Term
This enzyme phosphorylates glucose and is feedback inhibited by Glucose-6-Phosphate.
Definition
Hexokinase (as opposed to glucokinase)
Term
Protein malnutrition leads to what disease?
Definition
Kwashiorkor (as opposed to Marasmus from energy malnutrition)
Term
What are the net products in glycolysis?
Definition
2 Pyruvate, 2 ATP, 2 NADH, 2 H+, 2 H20
Term
When is NAD used?
Definition
Catabolic processes to carry reducing equivalents away as NADH
Term
What is the difference in presentation between von Gierke's disease and fructose intolerance?
Definition
Both have hypoglycemia, jaundice, cirrhosis and vomiting. von Gierke's disease also has lactic acidosis whereas fructose intolerance does not.
Term
Role of GTP in protein synthesis
Definition
GTP does tRNA Going places (aka translocation) and Gripping
Term
Role of P site in protein synthesis
Definition
P site accomodates growing Peptide.
Term
Disulfiram: Mechanism
Definition
Disulfiram inhibits acetaldehyde dehydrogenase, leading to an accumulation of acetaldehyde, leading to increased hangover symptoms.
Term
Where is Fructose 1-6 bisphosphatase found?
Definition
Liver and kidney
Term
How many ATP molecules are produced by aerobic metabolism of glucose?
Definition
38 via the Malate shuttle, and 36 via the G3P shuttle.
Term
Sequence of events in the termination step of protein synthesis.
Definition
1. Completed protein is released from ribosome. 2. Ribosome dissociates.
Term
Name the pyrimidines.
Definition
Cytosine, Uracil, Thymine
Term
Nucleotide excision repair: Mechanism
Definition
1. Specific endonucleases release the oligonucleotide containing damaged bases 2. DNA polymerase and ligase fill and reseal the gap, respectively
Term
What is this molecule an activated carrier of?: Thiamine Pyrophosphate
Definition
Aldehydes
Term
Pyruvate dehydrogenase deficiency: Mechanism
Definition
Backup of pyruvate and alanine leads to lactic acidosis.
Term
Hunter's Syndrome vs Hurler's Syndrome: Physical deformity?
Definition
Hunter's: Mild to severe, Hurler's: Dwarfing, coarse facial features, (gargoylism)
Term
What is the mechanism of ATPase inhibitors?
Definition
1. Directly inhibit mitochondrial ATPase causing: 2. Increased protein gradient and increased oxygen consumption, but no ATP is production
Term
True/False: Protein synthesis proceeds 5' to 3'
Definition
TRUE
Term
This enzyme phosphorylates glucose with high affinity.
Definition
Hexokinase (as opposed to glucokinase)
Term
What are the 5 cofactors necessary for pyrvuate dehydrogenase?
Definition
Lipoic acid plus the first four B vitamins in their active forms: 1. B1: TPP 2. B2: FAD 3. B3: NAD 4. B5: CoA
Term
What activated carriers carry: Aldehydes
Definition
Thiamine Pyrophosphate
Term
Ubiquitin or Ubiquinone: Proteosomal degradation
Definition
Ubiquitin
Term
In aerobic metabolism of glucose, which pathway produces 36 ATP?
Definition
G3P shuttle
Term
Where in the cell does the following occur: Heme synthesis
Definition
Pathway has steps in the mitochondria and in the cytoplasm
Term
DNA polymerase III: Which direction does it read?
Definition
3' to 5'
Term
What is the mechanism of CN?
Definition
Electron transport inhibition
Term
What are the 5 cofactors necessary for alpha-ketoglutarate dehydrogenase?
Definition
Lipoic acid plus the first four B vitamins in their active forms: 1. B1: TPP 2. B2: FAD 3. B3: NAD 4. B5: CoA
Term
Inhibitors of electron transport from FMNH2 to Coenzyme Q?
Definition
Amytal and Rotenone
Term
Where in the cell does the following occur: Glycolysis
Definition
Cytoplasm
Term
Ubiquitin or Ubiquinone: Coenzyme Q in oxidative phosphorylation
Definition
Ubiquinone
Term
What are the steps in processing hnRNA to make mRNA? (Note: This is more than splicing.)
Definition
1. Capping on 5' end with 7-methyl-G 2. Polyadenylation on 3' end (approximately 200 As) 3. Splicing out of introns
Term
Which position on the codon is the wobble position?
Definition
3rd position
Term
Prokaryotic genome: Describe DNA replication
Definition
Continuous bidirectional DNA synthesis on leading strand and discontinuous (Okazaki fragments) on lagging strand
Term
DNA Topoisomerase II: Mechanism
Definition
cuts both strands, and passes an unbroken double strand through it then reanneals the cut strand
Term
Eukaryotic genome: single/multiple origins of replication
Definition
multiple
Term
Eukaryotic genome: Trigger for replication
Definition
Consensus sequence of AT-rich base pairs
Term
Enzyme function: Primase
Definition
Makes an RNA primer on which DNA polymerase III can initiate replication
Term
What does eukaryotic RNA polymerase III make?
Definition
tRNA
Term
What does eukaryotic RNA polymerase I make?
Definition
rRNA
Term
Define promoter of gene expression.
Definition
Site where RNA polymerase and multiple other transcription factors bind to DNA upstream from gene locus
Term
What characterizes a promoter of gene expression?
Definition
AT-rich upstream sequence with TATA and CAAT boxes
Term
Amino acid binding to tRNA: Where (on the tRNA) and how?
Definition
Where: 3' end How: Covalently
Term
Special points about RNA polymerase II
Definition
1. Opens DNA at promoter site 2. Inhibited by alpha-amanitin
Term
Which RNA polymerase makes tRNA?
Definition
eukaryotic RNA polymerase III and prokaryotic RNA polymerase
Term
Which group is more likely to have G6PD deficiency?
Definition
Blacks
Term
What activates pyruvate dehydrogenase?
Definition
1. Increased NAD/NADH ratio (We need more NADH.) 2. Increased ADP (We need more ATP.) 3. Ca2+ (More of me leads muscles to contract)
Term
What is this molecule an activated carrier of?: NADPH
Definition
Electrons
Term
What activated carriers carry: Electrons
Definition
1. NADH 2. NADPH 3. FADH2
Term
What are the consequences of the altered NADH/NAD ratio seen in alcoholics?
Definition
Short-term: Hypoglycemia, Long-term: Hepatic fatty change
Term
What is this molecule an activated carrier of?: FADH2
Definition
Electrons
Term
What is this molecule an activated carrier of?: Tetrahydrofolate
Definition
1-carbon units
Term
What are the net reactants in glycolysis?
Definition
Glucose, 2 Phosphates, 2 ADP, 2 NAD
Term
What activated carriers carry: Phosphoryl
Definition
ATP and GTP
Term
What is this molecule an activated carrier of?: Biotin
Definition
CO2
Term
Nonhomologous end joining: Mechanism
Definition
Brings together two ends of DNA fragments (no requirement for homology)
Term
Mismatch repair: Mechanism
Definition
1. Unmethylated, newly synthesized string is recognized 2. Mismatched nucleotides are removed 3. Gap is refilled and resealed
Term
True/False: DNA is synthesized 3' to 5'
Definition
FALSE
Term
What is glutathione used for?
Definition
Detoxification of free radicals and peroxides.
Term
What are exceptions to universality of genetic code?
Definition
Mitochondria, Archaebacteria, Mycoplasma, and Some Yeasts
Term
What does it mean for genetic code to be universal?
Definition
Genetic code is conserved throughout evolution
Term
What is on the 3' end of a nucleotide
Definition
Hydroxyl group
Term
Types of DNA repair
Definition
Single stranded: Nucleotide excision repair, Base excision repair, and Mismatch repair; Double Stranded: Nonhomologous end joining
Term
DNA polymerase I: Which direction does it proofread?
Definition
5' to 3'
Term
True/False: DNA is synthesized 5' to 3'
Definition
TRUE
Term
DNA polymerase III: Which direction does it proofread?
Definition
3' to 5'
Term
Enzyme function: DNA polymerase III
Definition
Elongates the chain
Term
Hunter's Syndrome vs Hurler's Syndrome: Severity?
Definition
Hunter's: Less Hurler's: More
Term
What enzyme is missing in hereditary fructose intolerance?
Definition
Aldolase B
Term
What enzyme converts UDP-glucose to UDP-galactose?
Definition
Uridyl transferase
Term
What is the etiology of essential fructosuria?
Definition
Defect in fructokinase leading to lack of metabolism of fructose. Benign and asymptomatic
Term
When is genetic code not commaless/nonoverlapping?
Definition
In some viruses
Term
Which amino acids are acidic?
Definition
Aspartate and glutamate are negatively charged at body pH
Term
What is the immediate substrate for glycogen synthesis?
Definition
UDP-Glucose
Term
How does lactose intolerance present?
Definition
1. Bloating 2. Cramps 3. Osmotic diarrhea
Term
Hunter's Syndrome vs Hurler's Syndrome: Inheritance?
Definition
Hunter's: X-linked Recessive, Hurler's (and all other mucopolysaccharidoses): Autosomal recessive
Term
How many nucleotides does tRNA contain?
Definition
75 to 90 nucleotides
Term
Pyruvate kinase: What stimulates it?
Definition
Fructose-1,6-BP (I was told we needed more ATP, so here I am, so you better move the line along.)
Term
What enzyme converts galactose to galactitol?
Definition
Aldose reductase
Term
Ethanol metabolism: Order of kinetics of alcohol dehydrogenase
Definition
Zero-order kinetics
Term
What are the important products of the HMP pathway?
Definition
2 NADPH, Ribose, and glyceraldehyde-3-Phosphate and Fructose-6-phosphate
Term
What activated carriers carry: CO2
Definition
Biotin
Term
What reacts to yield S-adenosyl-methionine?
Definition
ATP and methionine
Term
What does it mean for genetic code to be unambiguous?
Definition
Each codon specifies only one amino acid.
Term
What does it mean for genetic code to be commaless?
Definition
Read from a fixed starting point as a continuous sequence of bases
Term
Define enhancer of gene expression.
Definition
Stretch of DNA that alters gene expression by binding transcription factors. May be located anywhere.
Term
When is NADPH used?
Definition
1. Anabolic process (steroid and fatty acid synthesis) 2. Respiratory burst 3. P-450
Term
Zinc deficiency: Presentation
Definition
Delayed wound healing, hypogonadism, and decreased adult hair (axillary, facial, pubic)
Term
What does transketolase require?
Definition
Thiamine (Vitamin B1)
Term
What does aldose reductase do?
Definition
Converts galactose to galactitol
Term
What protection does G6PD deficiency provide?
Definition
Protection against malaria
Term
Zinc deficiency: Predisposes to what?
Definition
Alcoholic cirrhosis
Term
What inhibits isocitrate dehydrogenase?
Definition
ATP and NADH
Term
What is the mechanism behind chronic fatty change in alcoholics?
Definition
1. Ethanol metabolism leads to an increased NADH/NAD ratio in the liver. 2. This ratio prefers fatty acid synthesis over glycolysis.
Term
What is this molecule an activated carrier of?: Lipoamide
Definition
Acyl
Term
How much ATP is used in the pentose phosphate shunt?
Definition
None
Term
What reaction does citrate synthase catalyze?
Definition
Oxaloacetate and acetyl coA combine to yield citrate.
Term
What is the etiology of lactose intolerance?
Definition
Loss of brush-border lactase
Term
mRNA initiation codons
Definition
1. AUG (inAUGurates protein synthesis) 2. GUG (rarely)
Term
What does Uridyl transferase do?
Definition
1. converts UDP-glucose to UDP-galactose 2. converts Galactose-1-Phosphate to Glucose-1-Phosphate
Term
What enzyme converts UDP-galactose to UDP-glucose?
Definition
4-epimerase
Term
Chromatin structure: What histones are included and which of these are not in the nucleosome core?
Definition
H1 (only one not in the core), H2A, H2B, H3, and H4
Term
Heterochromatin or Euchromatin: Which is more condensed?
Definition
Heterochromatin. Euchromatin is less condensed.
Term
Which drug inhibits acetaldehyde dehydrogenase?
Definition
Disulfiram
Term
Marasmus: Clinical presentation
Definition
Tissue and muscle wasting, loss of subcutaneous fat, and variable edema
Term
Heterochromatin or Euchromatin: Which is transcriptionally inactive?
Definition
Heterochromatin
Term
Hunter's Syndrome vs Hurler's Syndrome: Which GAGs' degradation is affected?
Definition
Both: Dermatan sulfate and Heparan sulfate
Term
What is the result of promoter mutation?
Definition
Dramatic decrease in amount of gene transcribed
Term
Anode: What does it contain?
Definition
Cations
Term
Which post-translational modification involves glycosylation?
Definition
post-translational covalent alteration
Term
Phosphofructokinase-1: What stimulates it?
Definition
AMP (Hey, we need more ATP), Fructose-2,6-BP (The fact that I'm being made means there's tons of glucose.)
Term
Names of the steps in protein synthesis
Definition
1. Initiation 2. Elongation 3. Termination
Term
This enzyme phosphorylates glucose with a low capacity.
Definition
Hexokinase (as opposed to glucokinase)
Term
This enzyme phosphorylates glucose with low affinity.
Definition
Glucokinase (as opposed to hexokinase)
Term
Prokaryotic genome: single/multiple origins of replication
Definition
single
Term
Pyruvate dehydrogenase deficiency: Presentation
Definition
Lactic acidosis and neurologic defects
Term
What are Heinz bodies?
Definition
altered Hemoglobin precipitates within RBCs, found in G6PD deficiency
Term
Lipoamide or lipoate: Which carries aldehydes?
Definition
Lipoamide
Term
What happens in post-translational trimming?
Definition
removal of N or C terminal pro-peptides from zymogens to generate mature proteins
Term
Which RNA polymerase makes rRNA?
Definition
eukaryotic RNA polymerase I and prokaryotic RNA polymerase
Term
Define operator of gene expression
Definition
Site where repressors bind
Term
What is the mechanism of thermogenin?
Definition
Uncoupling protein OR UCP which is an uncoupling agent
Term
How much ATP is produced by the citric acid cycle per molecule of acetyl coA?
Definition
12 ATP.
Term
Anode: What does it attract?
Definition
Anions
Term
Pyruvate kinase: What does it do, and what stimulates and inhibits it?
Definition
Pyruvate kinase converts phosphoenolpyruvate to pyruvate, thereby producing two ATP.
Term
Inhibitors of electron transport from Cytochrome b to Cytochrome C?
Definition
Antimycin A
Term
What sequence does every tRNA share at the 3' end?
Definition
CCA along with a high percentage of chemically modified bases
Term
In nucleic acids, what kind of substitution is a transition?
Definition
TransItion = Identical type (Purine for purine or pyrimidine for pyrimidine)
Term
Where is thermogenin found?
Definition
Brown adipose tissue
Term
This enzyme phosphorylates glucose and is not feedback inhibited.
Definition
Glucokinase (as opposed to hexokinase)
Term
Cyanogen bromide cleaves peptides at which side of what residues?
Definition
C-terminal of methionine
Term
This enzyme phosphorylates glucose with a high capacity.
Definition
Glucokinase (as opposed to hexokinase)
Term
What is on the 5' end of a nucleotide
Definition
Triphosphate
Term
Chymotrypsin cleaves peptides at which side of what residues?
Definition
C-terminal side of tyrosine, phenylalanine, and tryptophan residues
Term
Cathode: What does it contain?
Definition
Anions
Term
What type of bonds hold the phosphoryls together in ATP, and how much energy are the bonds worth?
Definition
Phosphoanhydride bonds are worth 7 kilocalories per mole (but only between the alpha and beta and the beta and the gamma)
Term
In what condition is mismatch excision repair mutated?
Definition
Hereditary Nonpolyposis Colon Cancer
Term
What is this molecule an activated carrier of?: NADH
Definition
Electrons
Term
Where in the cell does the following occur: Protein Synthesis
Definition
Rough endoplasmic reticulum in the cytoplasm
Term
Heterochromatin or Euchromatin: Which is less condensed?
Definition
Euchromatin. Heterochromatin is more condensed.
Term
Where in the cell does the following occur: acetyl-CoA production
Definition
Mitochondria
Term
What are the requirements of PEP carboxykinase?
Definition
GTP
Term
Where in the cell does the following occur: Krebs cycle
Definition
Mitochondria
Term
Which steps in the citric acid cycle produce ATP?
Definition
None, however 1 GTP is produced from the conversion of Succinyl CoA to Succinate.
Term
Ethanol metabolism: Limiting reagent
Definition
NAD+
Term
What does NADPH deficiency in RBCs result in?
Definition
Hemolytic anemia
Term
Enzyme function: DNA polymerase I
Definition
Degrades RNA primer and fills in the gap with DNA
Term
In what condition is nucleotide excision repair mutated?
Definition
Xeroderma pigmentosa (dry skin with melanoma and other cancers)
Term
What does transketolase require?
Definition
Thiamine (Vitamin B1)
Term
Where in the cell does the following occur: Gluconeogenesis
Definition
Pathway has steps in the mitochondria and in the cytoplasm
Term
Role of A site in protein synthesis
Definition
A site holds incoming Aminoacyl tRNA.
Term
True/False: RNA polymerase does not proofread.
Definition
TRUE
Term
What is the treatment for fructose intolerance?
Definition
Decreased intake of both fructose and sucrose.
Term
Where in the electron transport chain do NADH and FADH2 release their electrons?
Definition
Complex I
Term
DNA polymerase III: Mechanism
Definition
1. Adds deoxynucleotides to the 3' end until it reaches primer of preceding fragment, 2. 3' to 5' exonuclease activity proofreads each nucleotide
Term
What does aldose reductase do?
Definition
Converts galactose to galactitol
Term
What is this molecule an activated carrier of?: Coenzyme A
Definition
Acyl
Term
What is the Cori cycle?
Definition
Lactate in muscle is shuttled to liver where it is turned into glucose.
Term
What is alternative splicing?
Definition
Rearrangement of exons to make unique proteins
Term
What is wrong with a mischarged tRNA
Definition
Reads the regular bond but inserts wrong amino acid.
Term
Chromatin structure: In the beads on a string analogy, what is the string and how long is it?
Definition
Histone H1 ties the nucleosomes together in a 30-nm fiber string
Term
What are the rate limiting steps of glycolysis?
Definition
1. Hexokinase (Glucose to G-6-P) 2. *Phosphofructokinase-1 (Fructose-6-P to Fructose-1,6-BP) 3. Pyruvate kinase (PEP to Pyruvate)
Term
Which groups are more likely to be lactose intolerant?
Definition
1. Blacks 2. Asians
Term
True/False: RNA is synthesized 3' to 5'
Definition
FALSE
Term
Where do GLUT4 receptors predominate over other GLUT receptors?
Definition
Adipose tissue and skeletal muscle
Term
What inhibits pyruvate dehydrogenase?
Definition
1. NADH (No more of me please) 2. ATP (likewise) 3. Acetyl CoA (ditto)
Term
Where does the pentose phosphate pathway happen?
Definition
Cytoplasm of Red Blood Cells, and in lactating mammary glands, liver, and adrenal cortex (all sites of fatty acid or steroid synthesis except RBCs)
Term
What vitamin is necessary for regeneration of S-adenosyl-methionine?
Definition
Vitamin B12
Term
What is the clinical presentation of essential fructosuria?
Definition
Fructose appears in the blood and urine
Term
What disease state is glycolytic enzyme deficiency generally associated with?
Definition
Hemolytic anemia
Term
Phosphofructokinase-1: What inhibits it?
Definition
ATP (don't need more of me), Citrate (my cycle is going well)
Term
When is NAD used?
Definition
Catabolic processes to carry reducing equivalents away as NADH
Term
Where does NADPH come from?
Definition
HMP shunt
Term
Name some oxidizing agents that someone with a G6PD deficiency is vulnerable to.
Definition
1. Fava beans 2. Sulfonamides 3. Primaquine 4. Antituberculosis drugs
Term
What disease process is due to a missing respiratory burst?
Definition
Chronic granulomatous disease
Term
DNA polymerase III: Which direction does it write?
Definition
5' to 3'
Term
What activated carriers carry: Acyl
Definition
1. Coenzyme A 2. Lipoamide
Term
What reaction does citrate synthase catalyze?
Definition
Oxaloacetate and acetyl coA combine to yield citrate.
Term
What toxin inhibits lipoic acid?
Definition
Arsenic
Term
Where and when does eukaryotic RNA processing happen?
Definition
In the nucleus after transcription
Term
What are the requirements of PEP carboxykinase?
Definition
GTP
Term
What protection does G6PD deficiency provide?
Definition
Protection against malaria
Term
What are the main products of the pentose phosphate shunt and their uses?
Definition
1. NADPH (for fatty acid and steroid synthesis) 2. Ribose-5-phosphate (for nucleotide synthesis) 3. G3P and F6P (glycolytic intermediates)
Term
Chondroitin Sulfate: Where found?/Distinguishing characteristic from other GAGs
Definition
Cartilage, tendons, ligaments, aorta. Most abundant GAG in body.
Term
Keratan Sulfate: Where found?/Distinguishing characteristic from other GAGs
Definition
Found in cartilage proteoglycan aggregates with chondroitin sulfate, and in cornea. Most heterogeneous GAG.
Term
ATP and methionine react to form what?
Definition
S-adenosyl-methionine
Term
What are Heinz bodies?
Definition
Altered Hemoglobin precipitates within RBCs, found in G6PD deficiency
Term
What is the rate-limiting enzyme in the Pentose phosphate pathway?
Definition
Glucose-6-Phosphate Dehydrogenase
Term
What is glutathione used for?
Definition
Detoxification of free radicals and peroxides.
Term
What disease results from NADPH oxidase deficiency?
Definition
Chronic Granulomatous Disease
Term
What is the sequence to make TG (VLDL) from 1,3 DPG?
Definition
1,3 DPG to Glyceraldehyde-3-P to DHAP to Glycerol-3-P + FA makes TG
Term
Why are alcoholics prone to gout?
Definition
Ketoacids and Lactic Acid compete w/ uric acid for excretion in the kidneys; so Uric Acid Accumulates!
Term
Name two proteins involved in the structure of macula adherens.
Definition
1. Desmoplakin 2.Keratin
Term
Name two proteins involved in the structure of zona adherens?
Definition
1. E-cadherins 2. actin filaments
Term
What is the regulator of fat synthesis?
Definition
Acetyl CoA Carboxylase
Term
Most bile salts are composed of what?
Definition
Cholic acid with glycine or taurine
Term
Which process utilizes the citrate shuttle?
Definition
Fatty acid synthesis
Term
Which are the essential fatty acids?
Definition
Arachidonic, linolenic and linoleic
Term
Which lipoprotein is most dense?
Definition
HDL (peripheral scavenger)
Term
Which component of ground substance is a polymer?
Definition
hyaluranic acid
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