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Mycology microscope photos
Differentiate between the various species of fungi based on both mold and yeast forms under the microscope.
52
Microbiology
Undergraduate 2
09/14/2010

Additional Microbiology Flashcards

 


 

Cards

Term

 

 

Tinea

 

Affects what area?

What type of infection?

Is this term specific?

Definition

 

affects the skin-
"dermatophyte"

dermato: skin    phyte: to grow

 

ringworm (beard, face, scalp, body, groin/"jock itch", feet/"athletes foot" and nails)

 

Tinea is simply a blanket term for fungus of the skin/hair

Term

 

 

 

 

Microsporum

Definition

 

 

 

Dermatophyte of the skin and hair.

 

Macroconidia: Numerous, thick-walled fusiform or spindle shaped, multiseptate, with a rough surface.

Microconidia: few or absent, with a small, club shape.

Term

 

 

 

 

Microsporum Canis

Definition

 

 

 

 

Zoophilic, common cause of ringworm in dogs and cats.

 

Fluoresces with Wood's light.

Term

 

 

 

 

Microsporum gypseum

Definition

 

 

 

Geophilic, loves the soil

 

dermatophyte

Term

 

 

 

 

Microsporum audouinii

Definition

 

 

 

Primary cause of tinea capitis in school children.

 

dermatophyte

Term

 

 

 

 

Trichophyton

Definition

 

 

 

 

A dermatophyte that infects the hair, skin and nails.

Term

 

 

 

Trichophyton

Definition

 

 

Involves both endothrix and ectothrix infections.  

A form of a dermatophyte infection.

Macroconidia: Few, smooth, thin walled, club-shaped.

Microconidia: Few to Numerous round to club shaped in

grape-like clusters.

Term

 

 

 

 

Epidermophyton

Definition

 

 

 

A dermatophyte infection involving the skin and nails.  

Macroconidia: Numerous, club-shaped, smooth-walled

with 2 to 4 cells.

Microcondia: NONE.

Chlamydoconidia are numerous.

Term

 

 

 

Dimorphic

Definition

 

 

 

Fungal organisms capable of both the mold phase and the yeast phase.

Term

 

 

 

 

Yeast Phase

Definition

 

 

 

 

Growth at body temperature, 35-37°C.

Term

 

 

 

 

Mold Phase

Definition

 

 

 

 

Fungal growth at 25°C.

Term

 

 

 

Blastomyces

dermatitidis

Definition

 

 

Is one of the systemic mycoses.

Causes a respiratory infection that sometimes spreads to the skin and bones.  

Transmitted through inhalation of conidia or hyphae.

Dimorphic.

In the mold phase, it looks like little lollipops.

Term

 

 

 

 

Coccidiodes immitis

Definition

 

MOST INFECTIOUS OF ALL FUNGI.

Is one of the systemic mycoses.

In infection, 60% have an asymptomatic, self-limiting respiratory infection.  Can disseminate to organs, bone, skin, and lymph nodes.

The infection can become an epidemic.

Inhalation of arthrospores in soil or enviroment.

Septate hyphae with arthroconidia having a

barrel shape.

 

Term

 

 

 

Histoplasma

capsulatum

Definition

 

One of the systemic mycoses.

Primarily a lung infection, often confused with TB.

Can disseminate to the lymph nodes, bone marrow, liver and spleen and 95% are asymptomatic.

Severity of disease related to size of inoculum.

Organism is an intracellular parasite and is found upon direct examination within the host's mononuclear cells.

Dimorphic tuberculate macroconidia.

Term

 

 

 

Paracoccidiododes

brasiliensis

Definition

 

One of the dimorphic, systemic mycoses.

Chronic granulamatous infection that begins in the lungs, lymphatics, skin and mucous membranes and can spread to the liver and spleen.

"Mariner's Wheel" shaped yeast buds with

Chlamydoconidia in mold form.

Term

 

 

 

 

Penicillium marneffei

Definition

 

An opportunistic, dimorphic fungus.

Most often found in the immunocompromised.

Focal cutaneous or mucocutaneous infection with possible dissemination and fatality.

Direct examination yields small, yeastlike cells with internal crosswalls and no budding.

Thermal conversion to yeast form may be necessary for ID. 

 

Term

 

 

 

Sporothrix shenckii

Definition

 

Dimorphic, subcutaneous mycoses.

Sporotrichosis.

"Rose Gardner's Disease" because it can be aquired from pricking the finger from a thorny plant, or bales of hay.

Fast growing conidiophores, with a flowerette apearence. Delicate, branching, septate hyphae with the conidium attached via a denticle.

Term

 

 

 

 

Sporotrichosis

Definition

 

 

 

A chronic subcutaneous infection with rare dissemination.

Is the disease form of Sporothrix shenckii.

 

DOES NOT involve the hair. (trich/o)

Term

 

 

 

 

General Characteristics of Fungi

Definition

 

-Non Motile

-Eukaryotic

-True cell wall containing chitin

-Cell membrane contains ergosterol

-Lacks chlorophyll

-Reproduce with spores

-Heterotrophs

Term

 

 

 

 

Heterotroph

Definition

 

 

 

An organism that uses organic carbon for growth by consuming other organisms.

Term

 

 

 

 

Autotroph

Definition

 

 

 

An organism that can use direct sources of energy, like sunlight, to produce organic substrates like carbon dioxide.

ie: plants

Term

 

 

 

 

Fungal cell walls contain...?

Definition

 

 

 

 

Chitin.

Term

 

 

 

 

Fungal cell membranes contain...?

Definition

 

 

 

 

Ergosterol, as opposed to cholesterol.

 

 

 


Term

 

 

 

 

Mycelium

Definition

 

 

 

 

A mass of hyphae

Term

 

 

 

 

Hyphae

Definition

 

 

 

 

Microscopic tubular structures.

Term

 

 

 

 

Vegetative Portion

Definition

 

 

 

 

 

Grows on the substrate and absorbs water and nutrients.

On a diagram, it would be the lower portion.

Term

 

 

 

 

Aerial Portion

Definition

 

 

 

 

Contains the fruiting bodies that produce reproductive structures called spores (or conidia).

On a diagram, it would be the upper portion.

Term

 

 

 

Zygospores

Definition

 

 

One of the three forms of sexual reproduction.

Produced from the fusion of the tips of two compatible hyphae.

 

Term

 

 

 

 

Basidiospores

Definition

 

 

 

One of the three forms of sexual reproduction.

Formed at the end of a club shaped cell.

Term

 

 

 

 

Ascospores

Definition

 

 

 

One of the three forms of sexual reproduction.

A sac like structure contains the haploid cells that can be found inside the sac, or released to the outside.

Term

 

 

 

 

Imperfect Fungi

Definition

 

 

 

 

Fungi capable of asexual spore production.

Term

 

 

 

 

Deuteromycota

Definition

 

 

 

 

Phyla of fungi only capable of asexual spore production.

Term

 

 

 

 

-"mycota"

Definition

 

 

 

 

Phylum

Term

 

 

 

-"mycetes"

Definition

 

 

 

 

Class

Term

 

 

 

Two main forms of asexual reproduction:

Definition

 

 

 

Sporangiophores


Conidia

Term

 

 

 

Sporangiospores

Definition

 

 

 

 

Produced by the phyla Zygomycota

Term

 

 

 

 

Conidia

Definition

 

Produced by the phyla:

Ascomycota

Basidiomycota

Deuteromycota

Term

 

 

 

 

Conidiogenesis

Definition

 

 

 

Asexual reproduction with a stalk like structure extending from the hyphae and terminating in a swollen vessicle.

-metullae

-phialides

-conidia (single or in chains)

Term
[image]
Definition

 

 

 

 

Conidiogenesis

Term

 

 

 

 

Aspergillus

Definition

 

 

 

Conidiophore with swollen vessicle.  

Often refered to as a "fungus ball infection".

Numerous species including niger, flavus and clavatus.

 

Term

 

 

 

 

Penicillium

Definition

 

 

 

Conidiophore with branching.

Brush like conidia at the end of penicillus.

Term

 

 

 

Dematiaceous

Molds

Definition

 

 

 

A general term meaning dark-colored.

Generally non-pathogenic, except for the black mold.

Can be superficial or subcutaneous.

Term

 

 

 

Mycetoma

Definition

 

 

 

A subcutaneous dematiaceous infection involving the bone structure and other subcutaneous tissues.

Involves abscesses, draining sinuses and granulomatous pus.

Term

 

 

 

 

Chromoblastomycoses

Definition

 

 

 

A localized infection of the underlying tissue that is characterized by chronic, hard tumor-like lesions (cauliflower like) on the feet and lower legs.

Term

 

 

 

 

Rhizoids

Definition

 

 

 

 

Tree-root like growth structure.

Term

 

 

 

 

Yeasts

Definition

 

 

 

 

Most frequently isolated fungi in the clinical laboratory.

Term

 

 

 

 

India Ink

Definition

 

 

 

 

Stain used to ID yeasts under the microscope.

Term

 

 

 

 

Candida albicans

Definition

 

Most frequently isolated yeast.  

Normal flora of the GI tract mucocutaneous areas.

Infections include Thrush, diaper rash, vulvovaginitis and onychomycosis.  Can become systemic.

Exhibits the unusual characteristic of being BOTH a yeast and a mold at 37°C and a yeast at 25°C.

Only yeast to produce a germ tube.

Term

 

 

Cryptococcus

neoformans

Definition

 

 

 

A round, encapsulated yeast common in pigeon, bat or bird droppings.

Direct microscopic exam shows a very striking irregular sized, spherical yeast cell, often with budding.

Term

 

 

 

Cryptococcus

neoformans

 

Definition
[image]
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