Term
Brain Heart Infusion Agar
(BHI) |
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Definition
Primary recovery media with cycloheximide and chloramphenicol antibiotics
Support growth of some slow growers, Histoplasma capsulatum and Blastomyces dermatitidis
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Term
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Definition
Differential agar used for identification of Candida albicans
Stimulates formation of conidia and chlamydospores |
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Term
Niger Seed Agar/
Bird Seed Agar
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Definition
Differential media
used for identification of
Cryptococcus neoformans
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Term
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Definition
Enchances sporulation
Good for slide cultures
Demonstrate pigment production of
T. rubrum |
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Term
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Definition
Differential media
Differentiates T. mentagrophytes (+) from T. rubrum (-)
and Cryptococcus neoformans (+) from other negative species
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Term
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Definition
Growth in <7 days
-Most saprophytes
-Zygomycetes
-Coccidius immitus
- Yeast (1-5 days) |
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Term
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Definition
growth in 7-10 days
-most cutaneous fungi (dermatophytes)
-some saprophytes
-sporothrix schenkii
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Term
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Definition
>10 days
-Subcutaneous dematiaceous fungi; Cladosporium
-Few dermatophytes
-Systemic dimorphic fungi
except Coccidiodes immitus
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Term
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Definition
| Turns red after two weeks if dermatophytes present |
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Term
Sabaroud's Dextrose Agar
(SABS) |
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Definition
General Isolation Media
All organisms except some fastidious dimorphic molds will grow |
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Term
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Definition
Raquet Hyphae
hyphae with club-shaped cells; larger end of one is attached to smaller end of another cell
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Term
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Definition
Favic chandeliers
Terminal hyphal branches that are irregular, broad, and moose-antler like
Characteristic of Trichophyton schoenleinii |
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Term
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Definition
Sprial Hyphae
most commonly seen in Trichophyton spp. |
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Term
blastospores/blastoconidia |
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Definition
| Budding forms produced by yeast |
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Term
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Definition
| formed through elongated blastoconidia that remain attached |
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Term
Chlamydospores/Chlamydoconidia |
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Definition
Round, thick-walled resistant spores.
Found at hyphal tip (terminal), on sides of hyphae (sessile), or within hyphal strand (intercalary) |
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Term
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Definition
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Term
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Definition
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Term
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Definition
Intercalary chlamydospores |
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Term
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Definition
Arthrospores/Arthroconidia
formed directly from hyphae by fragmentation through points of septation
Square, rectangular, or barrel-shapd |
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Term
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Definition
Small portion of colony + subsurface agar is dug out. Placed on slide in drop of LPCB.
May disrupt fruiting structures of molds |
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Term
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Definition
Clear cellophane tape pressed gently to colony surface. Stretched over drop of LPCB on slide.
Spore arrangement better preserved
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Term
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Definition
Used when tease mount or scotch tape prep can't ID
Made using SAB, CMA, or Potato Dextrose Agar
Method of choice for making permanent mounts
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Term
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Definition
Bleaching agent taken up into chitin of fungal cell wall
Mixed with KOH when examining nails
Requires fluorescent scope |
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Term
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Definition
Used to detect Cryptococcus neoformans
Usually for CSF specimens |
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Term
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Definition
Hair perforation test
Differentiates T. mentagrophytes (+) from T. rubrum (-)
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Term
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Definition
Used as a clearing agent for samples containing keratin (skin, hair, and nails) so hyphae can be seen more easily
Mostly to ID dermatophytes
Performed on direct specimen, not culture plate |
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Term
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Definition
Niger Seed Agar (Bird seed agar)
turns maroon or dark brown with
Cryptococcus neoformans |
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Term
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Definition
Urea Agar
Cryptococcus neoformans (+)
Trichophyton mentagrophytes (+)
T. rubrum (-) |
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