Term
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Definition
| DIRECT REPAIR ENZYME: Binds to Pyrimidine Dimer, and when activated with white light breaks down dimers and restores function. |
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Term
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Definition
| DIRECT REPAIR ENZYME: Repairs O6-methyl(nucleotide base) methylation which causes improper nucleotide pairing. Methyl Tranferase transfers the methyl group from the nucleotide to a Cystine Residue thus correcting the mispairing. |
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Term
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Definition
| DNA Base Excision Repair: Repair mechanism for deamination events. Recognizes and removes damaged bases leaving apurinic or a pyrimidinic (AP) site. |
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Term
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Definition
| DNA Base Excision Repair: DNA glycosylase removes damaged nucleotides leaving AP site then AP endonuclease cleaves phosphodiester backbone near AP site. DNA Polymerase I and ligase finishes repair. |
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Term
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Definition
| Nucleotide Excision Repair: Isolates and nicks lesion on the damaged DNA. Helicase, DNA POL I, and Ligase finish the repair. |
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Term
| E. coli UVR A Excinuclease |
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Definition
| Nucleotide Excision Repair: Recognizes Damage and binds with UVR B |
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Term
| E. coli UVR B Excinuclease |
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Definition
| Nucleotide Excision Repair: Nothing specific on Pond's slides. Judging from figure UVR B seems to hold Damaged DNA bent until UVR C arrives at lesion. |
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Term
| E. coli UVR C Excinuclease |
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Definition
| Nucleotide Excision Repair: Binds @ Lesion after UVR A is released. Nicks (cuts) Damaged DNA on both sides. Helicase, DNA POL, and Ligase finish the repair |
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Term
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Definition
| Essential in mismatch repair, as mismatch repair can only occur with hemimethylated DNA. Dam methylase methylates new strand thus removing possibility for repair. |
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Term
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Definition
| Mismatch Repair: MUT S recognized mismatch and is bound by MUT L |
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Term
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Definition
| Mismatch Repair: Binds MUT S to damaged DNA |
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Term
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Definition
| Mismatch Repair: MUT H binds hemimethylated GATC sites, and uses endonuclease activity to make a cut at lesion site. Depending upon where cut is made a combination of Helicase and Endonuclease will remove damaged DNA completely leaving Polymerase to finish repair. (see slide 39 for complete terms) |
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Term
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Definition
| Occurs after replication has started. Replication is blocked (eg Thymine Dimer). Pol jumps damaged site leaving gap. Parent Strand recombines to fill gap left. Gap left in parent is filled by Pol and Ligase. |
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Term
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Definition
| SOS Repair System: Lex A is a repressor preventing transcription of SOS genes. In order for SOS induction to occur, Lex A must be inactivated by Rec A protein. |
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Term
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Definition
| SOS Repair System: Rec A protein proteolyses (cleaves) Lex A repressor thus inducing SOS repair gene. |
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Term
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Definition
| Mechanism for repairing nucleotide mismatching directly (eg. Thymine Dimers or Methylation mismatching). Uses photolyase and methyl transferase. |
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Term
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Definition
| Repair for deamination events in which a small DNA segment needs repair. Glycosylase and AP endonuclease used. |
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Term
| Nucleotide Excision Repair |
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Definition
| Repair for much larger DNA lesion or distorted DNA. Requires Excinuclease, and UVR (A,B,C) excinuclease in E.coli. |
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Term
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Definition
| Repair mechanism dependent upon hemimethylated DNA which is only present for a short time after replication. MUT (S,L,H) required for repair. |
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Term
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Definition
| Entire Gene dedicated as a desperation repair strategy. Induced in response to DNA damage that blocks replication (so if other systems fail.....). It is an error prone system. |
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Term
| KRAS + PPNS35 Diester Repair |
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Definition
| This isnt a real thing.........., but I love you. |
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