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| an endonuclease that specifically recognizes a short sequence of DNA,usually 4 to 6 base pairs (bp) in length,and cleaves a phosphodiester bond in both DNA strands within this sequence |
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| Most of the DNA sequences recognized by restriction enzymes are |
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| Are obtained by cleaving the unrelated DNAs with the same restriction enzyme? (important for recombinant DNA) |
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| DNA synthesized from a messenger RNA mRNA template in a reaction catalyzed by the enzyme reverse transcriptase and the enzyme DNA polymerase |
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| a single-stranded polynucleotide of DNA or RNA that is used to identify a complementary sequence on a larger single-stranded DNA or RNA molecule |
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| a technique that uses an electrical field to separate molecules on the basis of size |
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| Because DNA contains negatively charged phosphate groups,it will migrate in an electrical field toward the... |
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| Staining with dyes such as __________ allows direct visualization of DNA bands under ultra-violet light. |
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| produced when DNA on a nitrocellulose blot of an electrophoretic gel is hybridized with a DNA probe |
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| produced when RNA on a nitrocellulose blot is hybridized with a DNA probe |
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| involves separating proteins by gel electrophoresis and probing with labeled antibodies for specific proteins |
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| What molecular technique can be used in AIDS diagnosis? |
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| a set of host cells (or phage) that collectively contain all of the DNA sequences from the genome of another organism |
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| an in vitro method that can be used for rapid production of very large amounts of specific segments of DNA |
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| variations among individuals of a species in DNA sequences of the genome |
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| What are some method to detect polymorphisms? |
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1) RESTRICTION FRAGMENT LENGTH POLYMORPHISMS (only works if mutation happens to be in the restriction site)
2) DETECTION OF MUTATIONS BY ALLELE-SPECIFIC OLIGONUCLEOTIDE PROBES
3) TESTING FOR MUTATIONS BY PCR
4) DETECTION OF POLYMORPHISMS CAUSED BY REPETITIVE DNA
5) DNA CHIPS |
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| two-dimensional gel electrophoresis separates proteins by? |
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| charge and size, viewed under fluorescent imaging device |
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