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-E. coli genome is circular ds DNA -isn't a chromosome -found in a structure called a nucleoid -some are linear |
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occurs when additional turns are introduced into the double helix (positive) or turns are removed (negative) -circular DNA responds by winding around itself for smaller spaces |
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40 to 50 supercoils around the protein core |
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-not absolutely essential under normal conditions -can be ultimately dispensed; but have much of their DNA in plasmid form |
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packed with genes, less than 5 MB |
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Definition
group of genes involved in a singled biochemical pathway and expressed in conjunction with one another -not all genes of an operon are related |
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minimum number of genes necessary to maintain a living cell |
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Definition
-300 genes -free living cell is capable of living on/in something |
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lateral or horizontal gene flow |
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Definition
transfer of genes between species, even between highly unrelated species -happens by means of conjugation, transduction, and transformation |
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-usually circular but some linear -many copies per organelle, and many organelles per cell so you can have thousands of copies per cell |
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how many genes are in organeller genome |
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Definition
-10-100 genes; majority for respiratory complex, tRNA, and rRNA -chloroplast have about 200 genes |
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Definition
-bacteria invaded eukaryotic cells and lived as endosymbionts -mitochondrial gene segments have been found in chloroplast genomes -eventually lost most of their genes |
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Why didn't all of the organeller genes get transferred to the nuclear genome |
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Definition
some proteins need to be translated in the organelle due to hydrophobic interactions that would be difficult in the cytoplasm |
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