Term
What is the % difference between individual humans that is used to distinguish them? |
|
Definition
|
|
Term
True or False: Blood Typing is more useful for exclusion of potential suspects |
|
Definition
|
|
Term
Certain regions in our genome have DNA sequences that repeat over and over again, what are these repeating units known as? |
|
Definition
Variable Number Tandem Repeats |
|
|
Term
What do you use to digest DNA and "cut out" VNTRs? |
|
Definition
|
|
Term
What technique do you use to analyze VNTRs? |
|
Definition
|
|
Term
Define DNA Fingerprinting |
|
Definition
An individual's unique banding pattern |
|
|
Term
VNTR sequences are how many nucleotides long? |
|
Definition
|
|
Term
How many times are the VNTR sequences repeated head to tail (in tandem)? |
|
Definition
|
|
Term
What are VNTRs with respect to the number of repeats? |
|
Definition
|
|
Term
|
Definition
|
|
Term
If a particular VNTR repeat is single locus where does it occur? |
|
Definition
|
|
Term
If a particular VNTR repeat is Multi-locus where does it occur? |
|
Definition
Occurs in multiple places |
|
|
Term
Where is the locus generally at? |
|
Definition
Non-coding regions -introns & between genes (extragenic) |
|
|
Term
True or False: The function of tandem repeats is unknown |
|
Definition
|
|
Term
What are the following synonyms for: Minisatellites Minisatellite DNA |
|
Definition
|
|
Term
What are the following synonyms for: Microsatellites Microsatellite DNA |
|
Definition
|
|
Term
|
Definition
|
|
Term
True or False: VNTRs are difficult to analyze Require moderate amounts of INTACT DNA |
|
Definition
|
|
Term
True or False: With Forensic Cases the VNTRs are sometimes degraded and the amount is often small |
|
Definition
|
|
Term
What is the time period to analyze VNTRs? |
|
Definition
|
|
Term
STR sequences are how many nucleotides long? |
|
Definition
|
|
Term
|
Definition
|
|
Term
Can PCR be used with STR? |
|
Definition
|
|
Term
True or False: Only a few copies of INTACT DNA are needed in degraded specimen with STR |
|
Definition
|
|
Term
What is the time range to analyze STRs? |
|
Definition
Can be automated and performed in HOURS |
|
|
Term
|
Definition
Federally maintained database of DNA obtained from crime scenes and convicted violent offenders |
|
|
Term
What does CODIS stand for? |
|
Definition
Combined DNA Index System |
|
|
Term
CODIS uses how many STR Loci on various chromosomes amplified by PCR? |
|
Definition
|
|
Term
True or False: CODIS also analyzes amelogenin locus (determines gender) |
|
Definition
|
|
Term
True or False: X & Y chromosome have different size PCR products |
|
Definition
|
|
Term
What does an Amelogenin locus determine? |
|
Definition
|
|
Term
PCR Products are separated by _____ by polyacrylamide gel electrophoresis |
|
Definition
|
|
Term
What analyzes size and fluorescence of DNA STRs? |
|
Definition
|
|
Term
|
Definition
Forensic cases Paternity testing Monitoring BM Engraftment |
|
|
Term
What is the Hardy-Weinberg equation? |
|
Definition
|
|
Term
|
Definition
Frequency of the appearance of A allele |
|
|
Term
|
Definition
Frequency of the appearance of a allele |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
What is the locus frequency calculation for a heterozygous pair? |
|
Definition
|
|
Term
What is the locus frequency calculation for a homozygous pair? |
|
Definition
|
|
Term
True or False: Improper collection of crime scene specimen may invalidate test results |
|
Definition
|
|
Term
True or False: 1 hair can implicate someone for a crime |
|
Definition
|
|
Term
What does sneezing and coughing do? |
|
Definition
|
|
Term
Mitochondrial DNA is ONLY what type of inheritance? |
|
Definition
|
|
Term
True or False: mtDNA is non-nuclear and single-stranded DNA |
|
Definition
|
|
Term
True or False: mtDNA is non-nuclear and double-stranded DNA |
|
Definition
|
|
Term
What are mtDNA genes involved in? |
|
Definition
Kreb's cycle for energy production |
|
|
Term
True or False: mtDNA is MORE stable than chromosomal DNA |
|
Definition
|
|
Term
What is the copy number of mtDNA? |
|
Definition
|
|
Term
True or False: mtDNA Hypervariable region which varies between individuals |
|
Definition
|
|
Term
What 2 things is mtDNA useful for? |
|
Definition
Identifying individuals in disasters "Cold Cases" |
|
|
Term
Y Chromosome analysis can only occur in what population? |
|
Definition
Males; Inherited from father |
|
|
Term
What are the 3 factors to be considered when comparing tests? |
|
Definition
Speed Cost effectiveness Power of Discrimination |
|
|
Term
What does SNPs stand for? |
|
Definition
Single Nucleotide Polymorphisms |
|
|
Term
What does AFLP stand for? |
|
Definition
Amplified fragment length polymorphisms |
|
|
Term
True or False: SSNPs don't affect gene product or gene regulation |
|
Definition
|
|
Term
What does SSNP stand for? |
|
Definition
Silent single nucleotide polymorphisms |
|
|
Term
How many markers are associated with SNPs? |
|
Definition
|
|
Term
|
Definition
|
|
Term
In AFLP what is DNA treated with? |
|
Definition
Restriction enzymes; Specific sequences amplified |
|
|
Term
Which is more costly SNPs or STR analysis? |
|
Definition
|
|
Term
Which type of technique involves single base pair polymorphisms? |
|
Definition
|
|
Term
What has a HIGH Power of Discrimination and a SLOW speed of Analysis? |
|
Definition
|
|
Term
What has a HIGH Power of Discrimination and a FAST speed of Analysis? |
|
Definition
|
|
Term
What has a LOW Power of Discrimination and a SLOW speed of Analysis? |
|
Definition
|
|
Term
What has a LOW Power of Discrimination and a FAST speed of Analysis? |
|
Definition
|
|
Term
STRs used in forensics are highly polymorphic. How many different alleles are there detected for each locus? |
|
Definition
|
|
Term
The polyacrylamide gel used to separate amplified DNA fragments can distinguish alleles that differ in how many nucleotides? |
|
Definition
|
|
Term
Jeffreys DNA fingerprinting method is known as what? |
|
Definition
Multi-locus fingerprinting (VNTR) |
|
|