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Molecular Basis of Inheritance
Campbell Chapter 16: DNA
27
Biology
11th Grade
02/13/2011

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Term
transformation
Definition
change in genotype and phenotype due to assimilation of external DNA by a cell
Term
bacteriophage
Definition
viruses that infect only bacteria
Term
phage
Definition
shortened name for a bacteriophage, or virus that infects only bacteria
Term
double helix
Definition
two strands twisted into a helix shape: structure of DNA
Term
semiconservative model
Definition
when DNA replicates each of the two daughter molecules will have one old strand and one new strand
Term
origins of replication
Definition
places on the DNA molecule where replication begins; only one site in bacteria, many in eukaryotes
Term
replication fork
Definition
Y shaped region where the new strands of DNA are elongating
Term
DNA polymerase
Definition
enzyme that catalyzes the elongation of new DNA at a replication fork; add nucleotides one at a time
Term
leading strand
Definition
DNA made from the 5 to 3 prime direction; continuous strand
Term
lagging strand
Definition
DNA synthesized away from the replication bubble; short segments that must later be joined together
Term
DNA ligase
Definition
enzyme that joins the Okazaki fragments (lagging strands) together into one continuous strand of DNA
Term
Okazaki fragments
Definition
lagging strand pieces of DNA that must be linked together
Term
primer
Definition
short section of RNA that allows DNA polymerase to initiate synthesis of the new DNA
Term
primase
Definition
enzyme that adds RNA nucleotides to make the primer
Term
helicase
Definition
enzyme that untwists the double helixat the replication fork
Term
single strand binding protein
Definition
proteins that keep the unpaired DNA strands apart as they are copied
Term
mismatch repair
Definition
fixes mistakes that are made when DNA is copied. (error rate of 1 in 10,000 bases)
Term
nuclease
Definition
enzyme the cuts out the damaged DNA
Term
excision repair
Definition
DNA repair that involves a nuclease to cut out the damaged DNA, DNA polymerase to add new nucleotides, and ligase the connect lagging strands
Term
telomeres
Definition
nucleotide sequences at the end of chromosomes that are cleaved off after every division; signals cell mortality
Term
telomerase
Definition
enzyme that catalyzes the lengthening of telomeres; present most often in cancerous cells
Term
Griffith
Definition
transformation of cells
Term
Avery
Definition
transformation agent was DNA
Term
Hershey and Chase
Definition
DNA, not protein, is the hereditary material
Term
Chargaff
Definition
ratio of nucleotides A=T, C=G
Term
Watson & Crick
Definition
Structure of DNA
Term
Messelson & Stahl
Definition
semiconservative method of DNA replication
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