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MMBIO 240 CH 11 EVANS
Chapter 11
34
Microbiology
Undergraduate 1
02/22/2011

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Term
Branch Migration
Definition
A process that forms heteroduplex DNA by moving down the paired chromatids
Term
Cre/Iox
Definition
type of site-specific recombination. Involves the targeting of a specific sequence of DNA and splicing it with the help of an enzyme called Cre recombinase.
Term
Crossing Over
Definition

Nonparental or recombinant gametes are the product of meiotic crossing over, or recombination.

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Term
Double-Strand Break Repair
Definition
Model of Homologous Recombination. 1. A double-strand break is introduced to DNA 2. During meiosis single strands are produced with 3'-OH ends 3. A D-loop is formed 4. DNA polymerase 5.The second 3'-end of the double-strand break is captured when the extended D-loop reaches the single stranded region on the other double-strand break end and can anneal 6. 2 Holliday junctions 7. Resolution of the double Holliday junction gives crossover and noncrossover outcomes.
Term
Gene Knockout
Definition
Disruption of gene activity by DNA transformation
Term
Homologous Recombination (HR)
Definition
Process in which double strand breaks (DSBs)are repaired. Essential process for repairing DNA breaks and fore ensuring correct chromosome segregation in meiosis. Needed to repair damage from x-rays, DNA gaps at replication forks, can restart replication forks and is needed to maintain telomeres.
Term
Ascus
Definition
A sac where all the products of a single meiosis are recovered together in most fungi.
Term
Gene Conversion
Definition
Irregular Segregation during meiosis.
1. Often associated with a reciprocal crossover of flanking genes
2. it is sometimes incomplete, giving a meiotic product that has two genotypes, with one or a few DNA mismatches known as heteroduplex DNA.
Term
Gene Replacement
Definition
All or part of the coding sequence is deleted and replaced with a selectable marker.
Term
Gene Insertion
Definition
A selectable marker is inserted into the coding region of a gene, thereby disrupting its activity
Term
hetroduplex DNA
Definition
is generated by base pairing between complementary single strands derived from the different parental duplex molecules; it occurs during genetic recombination
Term
Holliday junction
Definition
is a mobile junction between four strands of DNA.
Term
homologous crossing over
Definition
is an exchange of genetic material between homologous chromosomes- It usually occurs when matching regions on matching chromosomes break and then reconnect to the other chromosome.
Term
mitiotic crossing over
Definition
is a rare type of genetic recombination that may occur in some types of somatic cells during mitosis. It results in the production of homozygous allele combinations in all heterozygous genes that are located on that chromosome arm distal to the crossover. Thus genes that were previously recessive are expressed creating a new phenotype
Term
nonhomologous end joining
Definition
is a pathway that repairs double strand breaks in DNA. It is referred to as "non-homologous" because the break ends are directly ligated without the need for a homologous template in contrast to homologous which requires a sequence to guide repair
Term
negative selection
Definition
is where negative, rather than positive, traits of a species are selected for evolutionary continuance. It is generally not desirable, but may be caused by man-made conditions such as bad management
Term
site specific recombination
Definition
also known as conservative site specific recombination is a type of genetic recombination in which DNA strand exchange takes place between segments possessing only a limited degree of sequence homology. They are highly specific, fst and efficient, even when faced with complex eukaryotic genomes.
Term
strand invasion
Definition
The process in which the nucleoprotein complex (composed of the broken single strand DNA and the recombinase) searches and identifies a region of homology in intact duplex DNA. The broken single strand DNA displaces the like strand and forms Watson-Crick base pairs with its complement, forming a duplex in which each strand is from one of the two recombining DNA molecules
Term
transgenic
Definition
Is the process of introducing an exogenous gene-called a transgene- into a living organism so that the organism will exhibit a new property ad transmit that property to its offspring.
Term
What is RecA
Definition
is a strand exchange protein that binds DNA and promotes the early steps of strand exchange between homologous DNA sequences
Term
The Holliday model is based on what?
Definition
A pair of single strand breaks and involves a crossed strand Holliday junction that can move along the DNA strand by branch migration
Term
The Meselson-Radding model is based on what
Definition
A single strand nick but also involves formation of a Holliday junction.
Term
What method is used to determine the location of altered genes while trying to create gene knockouts?
Definition
Southern Blotting
Term
What method is used to determine the location of altered genes while trying to create gene knockouts?
Definition
Southern Blotting
Term
The ____ complex prepares double stranded breaks for homologous recombination and alters its activity at chi sites
Definition
RecBCD
Term
True or False- In a knock out mouse, a normal gene is replaced with a disrupted gene.
Definition
True
Term
Human gastrin is a hormone involved in acid secretion and trophic (and perhaps tumor-promoting) effects. You what to find out what happens when mice have the human version of this gene. The mouse you produce (with the human gastrin gene) is a:
Definition
knockout mouse
Term
Knockout mice are very useful in studies of gene function because...
Definition
they show what happens to the phenotype when a specific gene is nonfunctional.
Term
T or F- General recombination requires long regions of homologous DNA on both partners in the exchange, whereas site specific recombination requires only short specific nucleotide sequences, which in some cases need be present on only one of the exchanging partners.
Definition
True
Term
T or F- General recombination involves the physical exchange of DNA segments, which entails the breaking and rejoining of phosphodiester bonds in the DNA backbone.
Definition
True
Term
T or F- The RecA protein combines a site specific, single-strand nicking activity with an ATP dependent DNA helicase function that can unravel single -stranded "whiskers" off a duplex DNA molecule.
Definition
False- THis is true of the RecBCD protein. RecA protein is required for the pairing of homologous duplexes
Term
The SSB protein of E. coli melts out short hairpin helices in single strands by binding to the sugar-phosphate backbone and holding the bases in an exposed position.
Definition
True.
Term
The RecA protein binds both single-stranded and double-stranded DNA so that it can catalyze synapsis between them.
Definition
True.
Term
What does Chi sites do?
Definition
They are specific DNA sequences that locally stimulate RecBCD mediated homologous recombination in E. coli
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