Term
| What major event (in terms of cytoskeletal structure) occurs during S phase? |
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Definition
| Duplication of the centrosome and organization of the spindle |
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Term
| What are the 3 types of microtubules involved in the mitotic spindle and what does each bind to? |
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Definition
1. Astral - bind toward the outer part of the cell
2. Overlap - overlap with mitotic spindle from other side of the cell, bind with myosin later in mitosis
3. kinetochore - bind to DNA at the kinetochore |
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Term
| In relation to the kinetochore microtubules, how do they allow DNA to migrate toward the ends of the cell? |
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Definition
| Rapid loss of subunits at the + end (center) and slow flux at the minus end (MTOC end) during anaphase A |
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Term
| What is the mechanism for the movement of DNA toward the outside of the cell during anaphase? |
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Definition
An ATP drive motor protein that allows migration toward the - end (NOT dynein here, mitotic kinesin does this)
Mitotic kinesin drives rapid subunit disassembly at the + and toward the - end, pulling the chromosomes toward the MTOC's |
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Term
| Describe the mechanism of Anaphase B |
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Definition
1. Astral proteins bind to dynein that is anchored in the cell wall
2. Due to anchor, instead of moving along microtubules, this creates a pulling effect on the mitotic spindle that moves the whole thing toward the outside (cortex) of the cell. Meanwhile, the overlapping filaments promote synthesis of new tubules in the overlap zone, allowing them to remain overlapped during this pulling of the spindle. |
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