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Miscellaneous Gram Negative Bacilli
Study Guide Questions
39
Microbiology
Graduate
11/25/2012

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Term
Brucellosis Diseases
Definition
Bang’s disease, undulant fever, can be asymptomatic to debilitating, laboratory acquired infections
Term
Brucellosis Transmission:
Definition
contaminated milk, cheese, penetration of ocular or oral mucosa, abrasions of skin, inhalation
Term
Brucellosis Cultivation
Definition
grows on blood, chocolate and enriched media. Incubation for prolonged periods with high CO2
Term
Brucellosis Colony Morphology:
Definition
smooth, translucent colonies, nonhemolytic
Term
Brucellosis Gram stain
Definition
gram negative coccobacilli
Term
Brucellosis Identification:
Definition
oxidase and catalase +, non motile
Term
Brucellosis Diagnosis
Definition
febrile agglutinations
Term
Brucellosis Treatment
Definition
prolonged IV of doxycyline and aminoglycoside
Term
B. abortus
Definition
cattle
• Variable CO2 requirements
• Urease positive, H2S positive
• Inhibited by: thionine
Term
B. melitensis
Definition
sheep, goats
• CO2 not required
• Urease positive, H2S positive
Term
B. suis
Definition
pigs
• CO2 not required
• Urease positive (rapid), variable H2S
• Inhibited by fuchsin
Term
B. canis
Definition
dogs
• CO2 not required
• Urease positive (rapid), H2S negative
• Inhibited by fuchsin
Term
Legionella Diseases
Definition
: legionnaires disease (pneumonia, Pontiac fever)
Term
Legionella Habitat
Definition
: fresh water
Term
Legionella Diagnosis
Definition
: direct fluorescent antibody, DNA probe + PCR, urine antigen detection, serology
Term
Legionella cultivation
Definition
required L-cysteine. Media: BCYE- ground glass colonies
Term
Legionella Gram stain
Definition
: thin faintly staining gram negative bacilli
Term
Legionella Identification:
Definition
weakly catalase and oxidase positive, real ID via DFA
Term
Bordetella Diseases:
Definition
pertussis (whooping cough), milder respiratory disease, kennel cough
Term
Bordetella Media
Definition
: Bordet Gengou, Regan Lowe
Term
Bordetella Incubation
Definition
: aerobic, 7 days
Term
Bordetella Gram stain
Definition
: very small gram negative bacillus
Term
Bordetella Colony morphology
Definition
: small pearl-like
Term
Bordetella Identification
Definition
: DFA
Term
Bordetella vaccine
Definition
not permanent
Term
Francisella Diseases:
Definition
tularemia (acute febrile granulomatous disease transmitted via infected animals and insects)
Term
Francisella tularensis Media
Definition
enriched with cysteine or cystine (BYCE agar)
Term
Francisella tularensis Incubation:
Definition
prolonged (2 weeks)
Term
Francisella tularensis Colony morphology
Definition
: translucent, wet mucoid
Term
Francisella tularensis Gram stain
Definition
: faintly staining GN rods or CB
Term
Francisella tularensis Identification
Definition
non motile, obligate aerobe
Term
Bartonella Diseases
Definition
: trench fever, Bacteremia, endocarditis, cat scratch fever
Term
Bartonella Culture
Definition
: fresh media chocolate or blood
Term
Bartonella Gram stain
Definition
: GNB
Term
Bartonella Identification
Definition
: oxidase, catalase negative
Term
Afnia felis
Definition
used to think it caused cat scratch fever, GNB, grows on BYCE
Term
Causes of Atypical Pneumonia
Definition
Francisella tularensis, Legionella pneumophila and Helicobacter spp.
Term
Disadvantages of DFA
Definition
not available for all speices
Term
Advantages of DFA
Definition
sensativity up to 80%
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