Thick Hyaline shell

Clear color

amorphous

1 flattened side

~55X25u

Football shaped

Bile-stained shell

2 transparent polar plugs of mucus

Usually unembryonated

~50X25u

bile stained

double hyaline shell

embryonation starting

cortication

Thin, single shell

Amorpous mass

Bile stained

cortication

Single hyaline shell

2-8 developing embryo

~60X40u

Cyst

Trophozoite

Trophozoite

-lives in L. intestine

-15-30u

-directional pseudopod

-finely granular endoplasm

-50% ingest RBC

-1 nucleus (central karyosome and even peripheral chromatin)

Cyst

-infectious stage

-8-12u

-1-4 nuclei

-may contain

--glycogen vacuole

--chromatoidal bars

Morphological

-loost stools-Trophs

-formed stools-Cysts

Entamoeba coli

Endolimax nana

Iodamoeba butschlii

Entamoeba gingivalis

Trophozoite

-lives in L intestine

-20-35u

-blund, sluggish pseudopod

-muddy endoplasm

-ingested bacteria and debris

-1 nucleus (eccentric karyosome, uneven peripheral chromatin)

Cyst

-infectious stage

-15-20 u

-1-8 nuclei

-may contain chromatoidal bars

• Feces
• soil
• mature cyst
• ingested by human
• small intestine
• Excystation
• 8 trophozoites
• L. intestine
• Splits
• 1- chronic or carrier state-->Cysts
• 2-Acute state-->Trophs
• Feces

Entamoeba histolytica Life Cycle

(write out on separate sheet of paper)

(Start with feces)

Entamoeba gingivalis

Trophozoite only

-10-20 u

-Commensal in mouth

-only amoeba to ingest WBC

Trophozoite

-Cytostome/peristrome

-muddy cytoplasm w/ vacoules

-2 nucleus (micro and macro)

-~60X45

Cyst

-50x70

-cell wall

-muddy cytoplasm w/ vacoules

-2 nuclei (macro and micro)