Term
How are chromosomes organized in eukaryotes/prokaryotes? |
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Definition
Eukaryotes: Chromosomes are organized in a membrane bound nucleus. Prokaryotes: Found in a general region of the cell called the nucleoid. |
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Term
Why is large size a possible disadvantage for eukaryotes? |
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Definition
ATP and other important molecules cannot diffuse as quickly through a large volume. |
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Term
What are two benefits of having compartmentalized organelles? |
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Definition
1) Incompatible reactions can be separated i.e. fatty acids can be destroyed in one organelle and synthesized in another. 2) Chemical reactions become more efficient because reactants and enzymes are clustered together. Also, coupled reactions show increased efficiency. |
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Term
Explain the organization of the eukaryotic nucleus and its three structures. |
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Definition
1) The nuclear envelope is a double membrane with pores 2) The nuclear lamina is made of proteins that give structure to the nucleus. All chromosomes are bound to the nuclear lamina 3) Nucleolus manufactures RNA and ribosomal subunits for ribosomes |
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Term
What organelle does the ER attach to? |
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Definition
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Term
What are the functions of the rough and smooth ER? |
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Definition
Rough: Has ribosomes embedded in its surface that make proteins
Smooth: Makes and breaks down lipids, also houses calcium ions |
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Term
What is the purpose of the Golgi Apparatus? |
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Definition
Takes products from the rough ER and puts them in vesicles to ship around the cell |
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Term
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Definition
Detoxifies cell by breaking down H2O2 into H2O and O2 |
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Term
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Definition
Breaks down waste into reusable molecules |
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Term
What are pinocytosis and phagocytosis? |
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Definition
Pinocytosis: Taking up small particles
Phagocytosis: Taking up big particles that need to be broken down |
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Term
What is the structure of actin and its purpose? |
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Definition
Actin is polymerized by individual actin subunits in a double helix (each subunit is polar and new actins add to the + end).
Its purpose is to resist expansion forces and participates in cell movement, cytokinesis, move organelles around |
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Term
What protein works with actin to produce movement? |
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Definition
Myosin attaches to actin and causes actin to slide (the myosin head moves when ATP is used) |
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Term
Where do the four steps of cellular respiration occur? |
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Definition
1) Glycolysis: Cytosol
2) Linking Step: Mitochondrial matrix
3) Citric Acid Cycle: Mitochondrial matrix
4) ETC: Mitochondrial inner membrane |
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Term
What is consumed/produced in Glycolysis and where does the reaction enter the point of no return? |
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Definition
Consumed: Glucose, NAD+, ADP, H2O(?)
Produced: 2 net ATP, 2 net NADH, and 2 pyruvates
The glycolysis pathway can be shut off at the step where the second phosphate group is added to make a symmetrical molecule. Phosphofructokinase can be inhibited by ATP at a regulatory site. |
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Term
What happens in the linking step? What is produced/consumed? |
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Definition
Pyruvates travel from the cytosol to the mitochondrial matrix and are converted to Acetyl CoA. A CO2 is given off so the 3 carbon pyruvate becomes a 2 carbon molecule with CoA attached. An NADH is produced per pyruvate.
Consumed: Pyruvates, CoA, NAD+
Produced: CO2, 2 NADH (1 per pyruvate), Acetyl CoA |
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Term
What happens in the Citric Acid Cycle? What is consumed/produced?
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Definition
Acetyl CoA binds to an oxaloacetate molecule (CoA leaves and goes to the linking step again). The 6 carbon molecule is oxidized until it has 4 carbons left (2 CO2 molecules leave) and electrons are given to NADH and FADH2. An ATP molecule is produced per revolution per pyruvate, 2 total.
Consumed: Acetyl CoA, ADP, NAD+, FAD, H2O(?)
Produced: 4 CO2, 2 ATP, 6 NADH, 2 FADH2 (total from 2 pyruvates) |
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Term
What happens in the Electron Transport Chain? What is consumed/produced? |
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Definition
NADH and FADH2 travel to the inner mitochondrial membrane and donate electrons to the enzyme complexes I and II. Each time electrons are donated, the complexes shoot H+ into the inner membrane space. Complexes I, III, and VI do this but II doesn't. After I and II are finished, the electrons travel by the Q transport protein and go to III, do the same thing, travel by C to IV and reduce oxygen to water.
Consumed: NADH, FADH2, O2
Produced: H+ gradient, NAD+, FAD, H2O |
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Term
What is chemiosmosis? What is consumed/produced? |
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Definition
The H+ gradient built up by ETC allows ATP synthase (the only gateway for ions like H+ to enter the matrix again) to synthesize ATP from ADP. Turns the wheel and catalyzes ADP to ATP reaction.
Consumed: ADP
Produced: ATP |
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Term
Where do fats and proteins enter the energy production pathway?
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Definition
Fats can be broken into glycerol and fatty acids. Glycerol is an intermediate in Glycolysis while fatty acids turn into Acetyl CoA.
Proteins can be broken into amine groups (lost as urine), pyruvates, Acetyl CoA, and intermediates in the Citric Acid Cycle. |
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Term
Where do the production pathways of DNA, fats, and proteins start? |
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Definition
DNA: Early steps of Glycolysis
Fats: Acetyl CoA step
Proteins: Citric Acid Cycle |
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Term
What three characteristics define sugars? |
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Definition
1) Presence of a carbonyl group (C=O) and various -OH hydroxyl groups
2) Differing numbers of carbons
3) Spatial arrangement of molecule, particularly -OH |
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Term
What is the term for a bond between two monosaccharides and the two types? |
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Definition
Glycosidic Linkage, can be either alpha or beta. Beta linkage is harder to break than alpha. |
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Term
What kinds of polymers do plants/animals/insects/bacteria make? |
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Definition
Animals make Glycogen to store energy (mostly alpha bonds)
Plants make Starch to store energy (mostly alpha bonds, made of amylose and amylopectin)
Plants also make Cellulose for structure (mostly beta bonds and hydrogen bonds)
Insects make Chitin for structure
Bacteria use Peptidoglycan for structure |
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Term
What are three reasons why carbohydrates didn't play a role in the beginnings of life? |
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Definition
1) They can't catalyze reactions
2) They have no way to polymerize on their own
3) They cannot form complementary base pairs to transmit information |
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Term
What three kinds of roles do carbohydrates play? |
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Definition
1) Structure
2) Cell-cell recognition
3) Energy storage/metabolism
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Term
What is a hyper/hypo/isotonic solution?
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Definition
Hypertonic: The side of the membrane in question is high in solute concentration
Hypotonic: The side of the membrane in question is low in solute concentration
Isotonic: Same concentration |
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Term
What is an electrochemical gradient versus a concentration gradient? |
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Definition
Electrochemical gradients are the combination of ion charges and concentration on a side of the membrane. A concentration gradient is simply the amount of solute present on one side of the membrane. |
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Term
How can substances move into a membrane against an electrochemical gradient? |
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Definition
Active transport with energy from ATP overcomes the gradient i.e. sodium potassium pump (sodium goes out, potassium comes in). Secondary active transport uses a different gradient to power a transport such as Na+ gradient used to bring glucose into the cell against the gradient. |
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Term
Why is the plasma membrane so important? |
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Definition
Keeps harmful compounds out & allows for entry of needed compounds.
Reactants are in the same area & collide more frequently. So reactions are more efficient. |
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Term
How do monosaccharides differ in structure? |
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Definition
Aldose/ketose placement of Carbon group
Variation in # of Carbons
Different arrangement of hydroxl group
Alternative ring forms |
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Term
What is the difference between the two types of bonds that can happen between two monosaccharides? |
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Definition
Alpha-1,4-glycosidic linkages are easy to break by enzyme
where as
Beta-1,4-glycosidic linkages are hard to break by enzymes |
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Term
How do carbohydrates play the role involved in cell identity? |
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Definition
Carbohydrates in the form of oligosaccharides (short chain of sugars) are covalently bonded to glycoproteins that protrude from the plasma membrane of cells and indicate cell identity with their "sugar coating". |
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Term
How do carbohydrates play a role in cell structure? |
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Definition
Carbohydrates such as chitin, cellulose and peptidoglycan provide for cell structure in plants, fungi and bacteria through their beta-1,4-glycosidic linkages that do not have many enzymes that can break them. |
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Term
How do carbohydrates play a role in more complex molecules? |
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Definition
Carbohydrates form the carbon skeleton of some molecules or are directly part of the molecule, like ribose in nucleotides. |
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Term
How do carbohydrates play a role in chemical energy? |
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Definition
Carbohydrates play the important role of taking in energy from sunlight in photosynthesis as carbohydrates have higher potential energy with C-H and C-C bonds than CO2 due to an equal sharing of e- in C-H and C-C bonds.
The energy is stored in starch/glycogen and harnessed when those two are hydrolized through enzymes and converted into glucose. |
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Term
What are the 3 types of lipids? |
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Definition
Fats, Steroids, & Phospholipids |
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Term
What is the structure of fats? |
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Definition
Glycerol (a 3 Carbon molecule) linked to 3 fatty acids (carboxyl group with hydrocarbon chain)
Glycerol & fatty acids linked through ester linkage (dehydration of glycerol's hydroxl & fatty acid's carboxl group) |
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Term
What is the structure of steroids? |
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Definition
A polar R group, nonpolar 4 steroid ring & isoprene chain. |
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Term
What is the structure of phospholipids? |
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Definition
A polar head consisting of a polar/charged group, phosphate group, & glycerol group, and then 2 nonpolar fatty acid or isoprene chains |
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Term
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Definition
Micelles are tiny droplets/circlets that form when hydrophilic heads face the outside solution in a circular fashion and hydrophobic tails are forced together. |
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Term
What are phospholipid bilayers? |
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Definition
Phospholipid bilayers are 2 sheets of phospholipid molecules aligned together where the hydrophilic heads are facing the outside and the hydrophilic tails are in the inside. |
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Term
What differentiates which phospholipids form micelles and which form phospholipid bilayers? |
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Definition
Tail length. Phospholipids that have short tail length form micelles and ones that have long tail length form phospholipid bilayerse. These are formed spontaneously. |
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Term
Order these molecules by highest permeability across a membrane.
O2, Cl-, glucose, glycerol |
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Definition
1. O2 - small, nonpolar molecule
2. glycerol - small, uncharged, polar molecule
3. glucose - large, uncharged polar molecule
4. Cl- ion |
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Term
How can membrane proteins be amphiphatic? |
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Definition
Because they are made of amino acids which have R-groups that range from being highly polar to highly nonpolar. |
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Term
What are the 3 types of transport proteins? |
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Definition
Channels, Transporters & pumps |
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Term
How do ions move across a membrane? |
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Definition
Through ions channels & diffusion from an area of high concentration to an area of low concentration. |
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Term
What is the structure of an amino acid? |
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Definition
Amino functional group of NH2, carboxyl functional group of COOH, H atom and R group. |
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Term
What are the functions of proteins? |
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Definition
Transport
Defense
Signaling
Structure
Movement
Catalysis
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Term
Primary structure of protein? |
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Definition
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Term
Secondary Structure of protein? |
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Definition
Hydrogen bonds between the carbonyl oxygen and amino hydrogen.
Forms alpha helix and beta-pleated sheets. |
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Term
Tertiary Structure of Protein? |
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Definition
R groups and their interactions with each other and the peptide-bonded backbone.
1.Hydrogen bonds
2.Hydrophobic interactions
3. Van der Waals interactions: assymetrical minute charges
4. Disulfide covalent bonds
5. Ionic bonds |
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Term
Quaternary structure of protein? |
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Definition
protein with more than one polypeptide |
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Term
List these types of molecule from greatest to least permeability:
1) Ions 2) Small nonpolar 3) Large nonpolar/polar 4) Small polar |
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Definition
Small nonpolar, small polar, large nonpolar/polar, ions |
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