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microscopy
dissecting and compound microscopes
21
Biology
Not Applicable
01/31/2005

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Term
dissecting microscope
Definition
useful in studying surface structures of 3d objects. 2 light sources: mounted above for opaque, below for transparent objects
Term
how a dissecting micro works
Definition
reflected light bounces off the sample, passes through an objective lens, and then through a par of ocular lenses to your eyes. magnification range of 10x-30x
Term
compound micro
Definition
uses a double lens system. magnifies up to 1000x
Term
ocular lens
Definition
found in eyepiece, usually 10x in power
Term
objective lens
Definition
mounted on a rotating disk. 4x, 10x, and 40x
Term
arm
Definition
supoorting body of scope
Term
eyepieces/oculars
Definition
house the ocular lenses. 10x
Term
body tube
Definition
hollow tube providing the right optical distance between th eocular and objective lenses
Term
revolving nosepiece
Definition
the mount for the objective lenses; revolves to bring the various objective lenses into position for viewing object
Term
objective lenses
Definition
lenses of various sizes and magnification powers. lowest lens has shortest tube, but provides largest viewing diameter
Term
stage
Definition
platform on which the microscope slice is placed under the objective lens
Term
substage lamp
Definition
incorporated into the base of the microscope
Term
substage condenser
Definition
a lens systme used to focus the light beam up into the iris, and the slide
Term
iris diaphragm
Definition
regulates light intensity. controls amount of light transmitted through the slide
Term
coarse focus adjustment knob
Definition
adjusts the coarse focusing.
Term
fine focus adjustment knob
Definition
adjusts the fine focus
Term
monocular scope
Definition
look into the ocular with the eye that is opposite of writing hand
Term
binocular scope
Definition
first adjust the width of the two eyepieces, until the distance matches your eyes.
Term
parfocal
Definition
once the proper focus is adjusted for one objective lens, the miage should remain sharp when switching to another objective lens
Term
preparing a wet mount
Definition
1) place 1-2 drops of water on a slide
2) place coverslip over specimen slowly so there are no air bubbles
3) first examine under low power
4) go to a higher power to see specific objects
Term
depth of field
Definition
focusing on one part of an object causes the others to go out of focus, the lower the magnification, the greater the depth of field
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