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Definition
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major parts of the microscope you use for sight |
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Definition
ocular (eyepiece) and objective (nosepiece) |
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usual magnification of the ocular lens |
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Common magnifications of the objective? |
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What magnification do you use oil immersion for? |
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Definition
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What is the total magnification? |
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Definition
Mag(objective) x Mag(ocular) |
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What is the base structural part of the microscope that supports the basic frame? |
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Why do you use oil with an oil immersion lens? |
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Definition
oil prevents light loss due to diffraction |
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As magnification of the objective lens increases, the working distance _______. |
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What does a condenser do in BRIGHT FIELD MICROSCOPY? |
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Definition
directs light towards the objective lens |
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What does a condenser do in DARK FIELD MICROSCOPY? |
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Definition
directs light at OBLIQUE angles away from the objective, causing objects to appear white on a dark field |
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What is the purpose of the iris diaphragm? |
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Definition
it adjusts the diameter of the cone of light |
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Term
As you close down the diaphragm,
light intensity _______ (increases/decreases),
contrast _______ (improves, does not improve),
depth of field _________ (increases/decreases),
and it ________ (raises/lowers) resolution (with oil immersion lens). |
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Definition
decreases, improves, increases, limits |
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To improve resolution, _____ (lower/raise) D.
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To improve resolution, (increase/decrease) lambda
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To improve resolution, (increase/decrease) the numerical aperture |
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