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Micro: Chapter 4
Microbiology chapter 4 for Indiana State University
76
Microbiology
Undergraduate 2
09/18/2013

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Term
Prokaryote?
Definition
  • prenucleus
  • one circular chromosome, not in a membrane
  • no histones
  • no organelles
  • bacteria: peptidoglycan cell walls
  • Archaea: pseudomurein cell walls
  • binary fission
Term
Eukaryote
Definition
  • Paired chromosomes, in nuclear membrane
  • histones
  • organelles
  • polysaccharide cell walss
  • mitotic spindle

 

Term
Basic shapes
Definition
  • bacillus (rod-shaped)
  • cocuss (sperical)
  • Spiral
    • Spirillum
    • Vibrio
    • Spirochete
Term
Arrangements
Definition
  • Pairs: diplococci, diplobacilli
  • Clusters: staphylococci
  • Chains: streptococci, streptobacilli
Term
Glycocalyx
Definition
  • Outside cell wall
  • Usually sticky
  • Capsule: neatly organized
  • Slime layer: unorganized and loose
  • Extracellular polysaccharide allows cell to attach
  • Capsules prevent phagocytosis
Term
Flagella
Definition
  • Outside cell wall
  • Made of chains of flagellin
  • Attached to a protein hook
  • Anchored to the wall and membrane by the basal body
Term
Motile cells
Definition


  • Rotate flagella to run or tumble 
  • Move toward or away from stimuli (taxis)
  • Flagella proteins are H antigens
Term
Axial Filaments
Definition
  • Also called endoflagella
  • In spirochetes
  • Anchored at one end of a cell
  • Rotation causes cell to move
Term
Fimbriae
Definition
  • allow attachment 
Term
Pili
Definition
  • Facilitate transfer of DNA from one cell to another
  • Gliding motility
  • Twitching motility
Term
Cell Wall
Definition
  • Prevents osmotic lysis
  • Made of peptidoglycan (in bacteria)
Term
Peptidoglycan
Definition
  • Polymer of disaccharide:
    • N-acetylglucosamine (NAG)
    • N-acetylmuramic acid (NAM)
  • Linked by polypeptides in Gram-Positive bacteria
Term
Gram-Positive Cell Walls
Definition
  • Thick peptidoglycan
  • Teichoic acids
  • May regulate movement of cations
  • Polysaccharides provide antigenic variation
Term
Gram-Negative Cell Wall
Definition
  • Thin peptidoglycan
  • Outer membrane
  • Periplasmic space
Term
Teichoic Acids
Definition
  • Lipoteichoic acid links to plasma membrane
  • Wall teichoic acid links to peptidoglycan
Term
Gram-Negative Outer Membrane
Definition
  • Lipopolysaccharides, lipoproteins, phospholipids
  • Forms the periplasm between the outer membrane and the plasma membrane
  • Protection from phagocytes, complement, and antibiotics
Term
O polysaccharide
Definition
  • antigen present in the outer membrane of gram negative cells
  • examples include E. coli O157:H7
Term
Lipid A
Definition
  • An endotoxin present in the outer membrane of gram negative cells
Term
Porins
Definition
  • proteins that form channels through the outer membrane of gram-negative cells
Term
The Gram Stain Mechanism
Definition
  • Crystal violet-iodine crystals form in cell
  • Gram-positive
    • alcohol dehydrates peptidoglycan
    • CV-I crystals do not leave
  • Gram-negative
    • alcohol dissolves outer membrane and leaves holes in peptidoglycan
    • CV-I washes out
Term
Gram-Positive cell wall
Definition
  • 2-ring basal body
  • Disrupted by lysozyme
  • Penicillin sensitive
Term
Gram-Negative cell wall
Definition
  • 4-ring basal body
  • Endotoxin
  • Tetracycline sensitive
Term
Atypical cell walls
Definition
  • Acid-fast cell walls
  • Mycoplasms
  • Archaea
Term
Acid-fast cell walls
Definition
  • Like gram-positive cell walls
  • waxy lipid (mycolic acid) bound to peptidoglycan
  • Mycobacterium
  • Nocardia
Term
Mycoplasmas
Definition
  • Lack cell walls
  • Sterols in plasma membrane
Term
Archaea
Definition
  • Wall-less, or
  • walls of pseudomurein (lack NAM and D-amino acids)
Term
Damage to the Cell Wall
Definition
  • Lysozyme digests disaccharide in peptidoglycan
  • Penicillin inhibits peptide bridges in peptidoglycan
Term
Protoplast
Definition
  • wall-less cell
Term
Spheroplast
Definition
  • wall-less gram-positive cell
  • protoplasts and spheroplasts are susceptible to osmotic lysis
Term
L forms
Definition
  • wall-less cells that swell into irregular shapes
Term
The Plasma Membrane
Definition
  • Phospholipid bilayer
  • Peripheral proteins
  • Integral proteins
  • Transmembrane
  • Proteins
  • Contain enzymes for ATP production
  • Selective permeability allows passage of some molecules
Term
Fluid Mosaic Model
Definition
  • Membrane is viscous as olive oil
  • Proteins move to function
  • Phospholipids rotate and move laterally
Term
Selective permeability
Definition
  • allows passage of some molecules
Term
Chromatophores
Definition
  • Also called thylakoids
  • present in the plasma membrane
  • Photosynthetic pigments on foldings in the plasma membrane
Term
Damage to the plasma membrane
Definition
  • Damage done by alcohols, quaternary ammonium (detergents), and polymyxin antibiotics cause leakage of cell contents
Term
simple diffustion
Definition
  • movement of a solute from an area of high concentration to an area of low concentration
Term
Facilitated diffusion
Definition
  • solute combines with a transporter protein in the membrane
Term
Osmosis
Definition
  • the movement of water across a selectively permeable membrane from an area of high water to an area of lower water concentration
Term
Osmotic pressure
Definition
  • the pressure needed to stop the movement of water across the membrane
Term
Movement of materials across membranes
Definition
  • Through lipid layer
  • Aquaporins (water channels)
Term
Ative transport
Definition
  • requires a transporter protein and ATP
Term
Group translocation
Definition
requires a transporter protein and PEP
Term
Cyoplasm
Definition
  • The substance inside the plasma membrane
Term
The Prokaryotic Ribosome
Definition
  • Protein synthesis
  • 70s
    • 50s + 30s subunits
Term
Inclusions
Definition
  • Metachromatic granules (volutin)
  • Polysaccharide granules
  • Lipid inclusions
  • Sulfur granules
  • Carboxysomes
  • Gas vacuoles
  • Magnetosomes
Term
Metachromatic granules
Definition
  • Also called Volutin
  • Phosphate reserves
Term
Polysaccharide granules
Definition
  • energy reserves
Term
Lipid inclusions
Definition
  • energy reserves
Term
Sulfur granules
Definition
  • energy reserves
Term
Carboxysomes
Definition
  • Ribulose 1,5-diphosphate carboxylase for CO2 fixation
Term
Gas vacuoles
Definition
  • protein-covered cylinders
Term
Magnetosomes
Definition
  • Iron oxide (destroys H2O2)
Term
Endospores
Definition
  • Resting cells
  • Resistant to desiccation, heat, chemicals
  • Bacillus, Clostridium
  • Sporulation and Germination
Term
Sporulation
Definition
  • endospore formation
Term
Germination
Definition
  • Return to vegetative state
Term
Endospore formation
Definition
  1. Spore septum begins to isolate newly replicated DNA and a small portion of cytoplasm
  2. Plasma membrane starts to surround DNA, cytoplasm, and membrane isolated in step 1
  3. Spore septum surrounds isolated portion, forming forespore
  4. Peptidoglycan layer forms between membranes
  5. Spore coat forms
  6. Endospore is freed from cell
Term
Flagella and Cilia
Definition
  • Microtubules
  • Tublin
  • 9 pairs + 2 array
Term
Endocytosis
Definition
  • energy using process by which cells absorb molecules by engulfing them
Term
Phagocytosis
Definition
  • pseudopods extend and engulf particles
Term
Pinocytosis
Definition
  • membrane folds inward, bringing in fluid and dissolved substances
Term
Cytoplasm membrane
Definition
  • substance inside plasma and outside nucleus
Term
Cytosol
Definition
  • fluid portion of cytoplasm
Term
Cytoskeleton
Definition
  • microfilaments, intermediate filaments, microtubules
Term
Cytoplasmic streaming
Definition
  • Movement of cytoplasm throughout cells
Term
Ribosomes
Definition
  • Protein synthesis
  • 80S
    • Membrane-bound: attached to ER
    • Free: in cytoplasm
  • 70S
    • In chloroplasts and mitochondria
Term
Organelles
Definition
  • Nucleus
  • Endoplasmic Reticulum
  • Golgi complex
  • Lysosome
  • Vacuole
  • Mitochondrion
  • Chloroplast
  • Peroxisome
  • Centrosome
Term
Nucleus
Definition
  • contains chromosomes
Term
Endoplasmic Reticulum
Definition
  • also called ER
  • transport network
Term
Golgi complex
Definition
  • membrane formation and secretion
Term
Lysosome
Definition
  • Digestive enzymes
Term
Vacuole
Definition
  • Brings food into cells and provides support
Term
Mitochondrion
Definition
  • cellular respiration
Term
Chloroplast
Definition
  • photosynthesis
Term
Peroxisome
Definition
  • oxidation of fatty acids; destroys H2O2
Term
Centrosome
Definition
  • consists of protein fibers and centrioles
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