Term
|
Definition
- obligate aerobic
- microaerophilic aerobic (require oxygen, but not as much as obligates)
- facultatively anaerobic (can grow well aerobically, but if you remove oxygen, it can shift to anaerobic met.- most versitile)
- oxygen tolerant anaerobic (oxygen doesnt prevent it from growing)
- obligate anaerobic (must have oxygen free atmosphere)
|
|
|
Term
| Different nutritional requirements |
|
Definition
- growth on chocolate agar
- growth of 5% sheep blood agar
- erythrocytes still in tact
|
|
|
Term
| Different hemolytic properties |
|
Definition
- alpha hemolysis (Strep. pneumoniae)
- produces enzymes that partially lyse erthryocytes
- Hb has other enzymes acting on it making it turn green
- beta hemolysis (Strep pyogenes)
- clear zone of complete hemolysis
- gamma hemolysis (Enterococcus casseliflavus)
- doesn't lyse erthryocytes at all
- can grow from other nutrients that are present in the blood agar
|
|
|
Term
|
Definition
- Gram negative (doesnt retain crystal violet after discoloration with alcohol)
- Gram positive (retains crystal violet dye)
|
|
|
Term
|
Definition
- completely acid fast (mycobact.)
- used with mycobact. because of their lipid rich cell walls preventing proper gram staining
- partially acid fast (nocardia)
|
|
|
Term
| Various tests to ID bacteria |
|
Definition
- mode of metabolism
- nutritional requirements
- hemolytic requirements
- gram stain
- acid fast stain
- catalase test
- oxidase test
- biochemical tests
- DNA probles
|
|
|
Term
| Catalase test function and indication of positive test |
|
Definition
|
|
Term
|
Definition
- detects if organism produces cytochrome oxidase A3 (turns purple)
- FLASHBACK- function of enzyme is to reduces oxygen gas to water
|
|
|
Term
| biochemical tests: how can the be performed? |
|
Definition
- how it can be performed
- manually, individually performed
- automated
|
|
|
Term
| Role of DNA probes in bacteria ID |
|
Definition
- used when highly accurate ID of isolates is required
- ex: gonorrhea in victims of sexual assault
|
|
|
