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Microbiology
10-14
100
Biology
Undergraduate 3
03/20/2009

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Term
Phototrophs require what source of energy?
Definition
light
Term
Lithotrophs require what source of energy?
Definition
inorganic substances
Term
organotrophs require what source of energy?
Definition
preformed organic matter
Term
chemotrophs require what source of energy?
Definition
organic or inorganic matter
Term
All microbes require:
Definition
a source of energy, a source of water, macroelements, trace elements
Term
Macroelements become components of
Definition
lipids, proteins, and nucleic acids
Term
trace elements become components of
Definition
enzymes and cofactors
Term
Microbes which use CO2 as their main source of C are:
Definition
autotrophs
Term
Microbes that use more complex molecules for C are:
Definition
heterotrophs
Term
Nitrogen is required for the synthesis of:
Definition
nucleic acids, ATP, amino group (of amino acids), some lipids
Term
Iron is required for:
Definition
enzyme function and use in Electron Transport Chain
Term
What facilitates movement in nutrient uptake?
Definition
permeases
Term
T/F: Active uptake does not require energy
Definition
False!
Term
In Active transport, molecules are transported across the membrane against __ or __
Definition
Protonmotive force or concentration gradient
Term
Protonmotive force is:
Definition
a proton gradient
Term
T/F: In group Translocation, the molecules are chemically altered as they are being taken up
Definition
True
Term
Group Translocation involves
Definition
enzymes and membrane proteins
Term
Who usually uses group translocation?
Definition
G+ve and G-ve facultative anaerobes (enterobacteria)
Term
Non-siderophore mediated uptake involves what type of protein?
Definition
Transferrin binding proteins (TBPs)
Term
TBPs
Definition
interact directly with host transferrin/lactoferrin and dissociate the Fe from them
Term
A media whose exact composition is known:
Definition
Chemically defined media (CDM)
Term
T/F: Complex media's exact composition is known
Definition
False
Term
Complex media is used to grow:
Definition
Heterotrophs and fungi
Term
Media that allows only the growth of certain bacteria and suppresses the growth of others
Definition
Selective media
Term
A media that is used for the growth of obligate anaerobic bacteria
Definition
Anaerobic growth media-Reducing media
Term
A media that allows bacteria to be easily distinguished from others
Definition
Differential media
Term
Under optimal environmental/nutrient conditions many bacteria can produce a new generation every:
Definition
20-30 minutes
Term
Four typical phases of a growth curve:
Definition
Lap phase, log phase, stationary phase, death phase
Term
When first inoculated, growth doesn't occur immediately (bacteria synthesizing cellular components) this phase is known as:
Definition
Lag phase
Term
The phase when bacteria have exponential growth and are dividing at the max rate
Definition
Log phase
Term
The phase when growth stops, but cell function continues. Nutrients depleted, wastes accumulated
Definition
Stationary phase
Term
T/F: There is no net increase or decrease in cell number during the stationary phase
Definition
True
Term
The phase in which cells lyse and die
Definition
Death phase
Term
Bacteria whom grow between pH 0 and pH 5.5
Definition
Acidophiles
Term
Bacteria whom grow between pH5.5 and pH 8
Definition
Neutrophiles
Term
Bacteria whom grow between pH8.5 and pH 11.5
Definition
Alkalophiles
Term
Most pathogens are: Acidophiles, neutrophiles, or alkalophiles?
Definition
neutrophiles
Term
Toxic forms of Oxygen:
Definition
Singlet oxygen (02), Superoxide free radicals (O2-), Peroxide anion (O2^2-), Hydroxyl radical (OH-)
Term
Superoxide dismutase (SOD) enzyme converts
Definition
O2- to O2 and H2O2
Term
Catalase and Peroxidase enzymes neutralize
Definition
O2^2-
Term
T/F: Hydroxyl radical is the least reactive form of oxygen
Definition
False: most reactive form
Term
Pseudomonas and Micrococcus are: A) faculatative anaerobes B) obligate aerobes
Definition
B
Term
T/F: Obligate aerobes produce SOD and catalase enzymes
Definition
True
Term
E, coli, Salmonella, Pasteurella are: A) Facultative anaerobes B) obligate aerobes
Definition
A
Term
T/F: Faculatative anaerobes produce SOD and catalase enzymes
Definition
True
Term
Bacteroides and Clostridium are A) Faculatative anaerobes B) obligate anaerobes
Definition
B
Term
A bacteria that will tolerate the presence of oxygen but does not utilize it for growth
Definition
Aerotolerant anaerobes
Term
Streptococcus and Lactococcus are: A) obligate anaerobes B) aerotolerant anaerobes
Definition
B
Term
Heliopacter pylori and Campylobacter jejuni are: A) aerotolerant anaerobes B) microaerophiles
Definition
B
Term
Bacteria that grow at reduced oxygen tension and do not produce SOD or catalase
Definition
microaerophiles
Term
The killing of all viable microbes
Definition
Sterilization
Term
The killing or inhibition of microbial growth on non-living objects (not spores)
Definition
Disinfection/disinfectants
Term
Microbial population reduced to acceptable levels (not spores)
Definition
Sanitation
Term
Prevents microbial growth on living tissue (not spores)
Definition
Antisepsis/antiseptics
Term
Kills microbe, bactericide, fungicide
Definition
Germicide
Term
The sum of all chemical reactions in an organism
Definition
metabolism
Term
The removal of electrons from a molecule , which produces energy
Definition
oxidation
Term
The acceptance of electrons from a molecule
Definition
Reduction
Term
Chemical reactions that involve breaking bonds to release energy
Definition
Catabolism
Term
Chemical reactions that require energy and build complex molecules from simple ones
Definition
Anabolism
Term
The addition of phosphate to a compound is called:
Definition
phoshorylation
Term
The three ways phosphorylation can be acheived:
Definition
substrate-level phosphorylation, oxidative phosphorylation, and photophosphorylation
Term
Enzymes are mostly made up of:
Definition
proteins
Term
Proteins are made up of:
Definition
amino acids
Term
__ speed up chemical reactions within the cell
Definition
enzymes
Term
Enzymes speed up the chemical reactions within the cell by __
Definition
lowering the amount of energy required to drive a reaction (the activation energy)without changing the cell
s temp or pressure
Term
An enzyme that speeds up a chemical reaction without permanently altering themselves
Definition
catalyst
Term
Enzymes are: A)specific B) general
Definition
A
Term
The speed of the enzyme is referred to as:
Definition
turnover rate/ conversion rate
Term
The specific spatial configuration of surface amino acids and where an enzyme interacts with the substrate
Definition
Active site
Term
Enzymes that are made up of proteins and non-proteins
Definition
Holoenzymes
Term
Holoenzymes are made up of:
Definition
apoenzyme and cofactor
Term
The apoenzyme is the __ portion of the holoenzyme
Definition
protein
Term
The cofactor portion of the holoenzyme is made up of
Definition
Non-protein things (metal ions and coenzymes)
Term
T/F: the apoenzyme does not need the cofactors to function
Definition
False!
Term
Name two important enzymes
Definition
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+)
Term
NAD+ is involved in: A) catabolic reactions B) Anabolic reactions C) both
Definition
A
Term
NADP+ is involved in: A) catabolic reactions B) Anabolic reactions C) both
Definition
B
Term
NAD+ and NADP+ is derived from:
Definition
Vitamin B (niacin)
Term
Co-enzyme A is important for __
Definition
the breakdown of fats
Term
T/F: Low Km = high substrate []
Definition
False
Low km= low substrate []
Term
An inhibitor that interacts with the enzyme's active site and completes with native substrate
Definition
Competitive
Term
Inhibitors interact with the allosteric site on the enzyme (not the active site)
Definition
Noncompetitive
Term
The mechanism by which the cell controls the amount of energy used and chemical reactions needed to make a given product
Definition
Feedback inhibition
Term
Enzymes regulated by addition or deletion of small organic molecules
Definition
Modification of enzymes
Term
T/F: All members of the Enterobacteria are catalase positive
Definition
True
Term
T/F: All strains of the Pasteurella multocida are lactase positive
Definition
False: lactase negative
Term
To obtain energy, microorganisms use what two processes?
Definition
Cellular respiration and fermentation
Term
Cellular respiration and fermentation both start with __
Definition
glycolysis
Term
Glucose can be taken up by bacteria via either __ or __
Definition
Active transport or Phosphotransferase System (PTS)
Term
T/F: In PTS, as the molecule comes in, it is altered
Definition
True
Term
What enters the glycolysis cycle?
Definition
glucose-6-phosphate
Term
In glycolysis, glucose-6-phosphate is oxidated to
Definition
pyruvic acid/pyruvate
Term
T/F: Glycolysis requires oxygen
Definition
False
Term
6-carbon glucose becomes __
Definition
3 carbon pyruvate + 2 ATP and 2 NADH
Term
Pentose Phosphate Pathway (PPP) is the __ of __ and produces __ ATP
Definition
oxidation of 5-carbon molecules; 1
Term
Entner-Doudoroff Pathway uses __ and produces _ ATP
Definition
coenzyme NADPH; 1
Term
The Entner-Doudoroff Pathway is used by: A) gram negative B) gram positive
Definition
A
Term
When the final electron receptor is O2
Definition
aerobic respiration
Term
When the final electron receptor is a number of molecules other than O2
Definition
Anaerobic respiration
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