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Microbiology
Lecture Test 2 Packet
72
Biology
Undergraduate 2
02/24/2009

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Cards

Term
Describe the Endosymbiotic Theory
Definition
The question that asks if a small aerobic bacterium and a larger anaerobic hetertrophic bacterium entered into a symbiotic relationship, where the cell enveloped the smaller cell by phagocytosis. Instead the smaller cell being digested though, could the 2 organisms have entered into a situation where the 2 bacteria became dependant upon each other and formed the mitochondria?
Term
What is the evidence that supports the Endosymbiotic theory?
Definition

1. Mitochondria have their own circular loop of DNA

2. Mitochondria reproduce independently

3. Mitochondria have 70s Ribosomes and make their own proteins

4. Mitochondira have double membrane (inner could be from the small aerobic bacterium and the outer one could be from the cell that phagocytized it

 

This could also explain the origins of chloroplasts in algae that theoretically evolved into plants

Term
What is the autogenous theory?
Definition
The theory that asks the question: could the endomembranous system of organelles (Golgi bodies, Endoplasmic Reticulum, nucluear membrane) be self generated through invaginations of the cell membrane?
Term

What type of microbiologists study the following

Protozoa

Algae

Fungi

Inverebrate Animals

Definition

Protozology

Phycology

Mycology

Parasitology

Term
The Kingdom Protista constist of...
Definition

Eukaryotes and Unicellular

 

Subkingdom: Protozoa (eukaryotes, unicellular, hetertrophs)

 

Subkingdom: Algae (eukaryotes, unicellular (colonial) autotrophs

Term
How are protozoa classified?
Definition
by their method of motility
Term
Describe the Phylum Sarcodina
Definition

-subkingdom Protozoa 

 

- amoeba

 

- use psuedopods for movement

 

- ex: Naegleria fowleri

Entamoeba histolytica

Term
Describe the phylum Mastigophora
Definition

subkingdom Protozoa

 

-use flagella for motility

 

ex: Trhciomonas vaginalis

Giardia lamblia

Chaga's disease

Term
Describe phylum Ciliophora
Definition

- use cilia for motility (paramecia)

 

-hairlike

 

-ex: Balantidum coli

 

Term
Describe phylum Apicomplexa
Definition

- they have no independent means of motility

(at the mercy of body fluid for movement)

 

-only on class in this phylum: sporozoa

 

-ex: plasmodium spp. (malaria

Toxoplasma gondii

Cryptosporidium parvum

Term

How are algae classified?

Definition

By their pigments

 

Green Algae - ancestors of land plants

Red Algae- source of agar

Brown Algae - kelp and other large ocean seaweeds

Golden Algae - Makes massive amounts of 02 gas

Fire Algae - dinoglagellates cause red tide, bioluminsce

Euglena - can be autotrophs or hetertrophs (hetertrophs when there is no sunlight

Term
Why are fungi important? (3 main reasons)
Definition

1. they are decomposers

2. they produce antibiotics

3. serve as a source of food (saphrophytes)

Term
What are diseases of fungi called?
Definition
mycoses or mycotic infections
Term
What is dermatomycoses?
Definition
skin fungal infection
Term

What does superficial dermatomycoses mean?

Definition
surface of the skin or mucous membranes
Term
What is candidiasis?
Definition

Candida albicans - part of your normal flora

 

thrush-oral

vaginal yeast infections

Term

What is tineas?

What are the 3 possible genera?

Definition

Ringworm

 

Trichophyton - hari skin nails

Microsporum - hair aor skin

Epidermophyton - skin or nails

Term
Is Ringworm a worm?
Definition
No, it's a fungus
Term
What is Tinea corporis?
Definition
Ringworm of the body
Term
What is tinea capitis?
Definition
Ringworm of the head
Term
What is Tinea cruris?
Definition
jock itch
Term
What is Tinea pedis?
Definition
athletes foot
Term
What is Tinea unguium?
Definition
nail fungus
Term
How can you diagnose Ringworm?
Definition

- Sabourad's dextrose Agar

 

- Skin scraping in KOH (will show branch like structure)

 

-woods lamp (flourescent light) some glow in the dark

Term
What are fungal infections due to puncture wounds?
Definition
subcutaneous mycoses
Term
What is an example of a subcutaneous mycoses?
Definition
Sporothrix schenkii (gardners)
Term

What are inhaled fungal infections?

 

Example:

Definition

Deep systemic mycoses

 

Histoplasma capsulatum (histoplasmosis)

 

Cryptococcosis, Blastomycosis, Coccioidomycoses

Term
What are three types of Helminths?
Definition

Flatworms (playtehelminths)

 

Roundworms (nematodes)

 

Segmented worms (annelids)

Term
What are some examples of Flatworms?
Definition

Tapeworms (from undercooked or raw beef, pork, fish)

 

Liver and Lung Flukes - goes through specific cycle: human, snail, crayfish, human

Term
What are some examples of Roundworms?
Definition

Pinworms (peri-anal itching) - transmitted anal-oral

 

Hookworms - larvae go through skin. Barefee increase rate of transmission

 

Heartworms - dogs and cats get from mosquitos

Term
What is an example of segment worms?
Definition
leeches - used in medicine. Anticoagulent and antisthetic in saliva
Term
Plasmodium vivax and other Plasmodium spp.
Definition

phylum Apicomplexa

Vector: Anopheles mosquito

Cause: Malaria

symptoms: chills, fever, headache, vomiting

-kills 1,000,000 people worldwide each year

Term
Borrelia burgdorferi
Definition

-etiologic agent: Lyme disease

 

trasmission: deer tick

 

sighns: classic bulls eye rash

Term
Rickettsia ricketsii
Definition

Cause: Rocky Mountain Spotted Fever

Vector: Tick

-small animals act as resevoir (rodents, chipmunks, squirrels)

-most cases occur in easter and southeastern US

-sudden headaches, fever, rash, may lead to death if untreated

Term

Rickettsia prowazekii

 

Definition

etiologic agent of typhus

 

-Vector: human louse

 

-high fever, frontal headache, muscle pain, rash

 

-common during wars, close quarters of uncleanliness, concentration camps

Term
Yersinia pestis
Definition

etiologic agent: the plague

vector: rat flea (small woodland creatures)

 

Infection of the lymph node: bubonic plague

Infection of circulatory system: black plague

Inhaled infection: Pneumonic plague

 

-black come from black spts on their skin from clogged and broken capillaries

Term
Viral encephalitis
Definition

St. Louis, EEE, WEE, VEE, West Nile

 

vector: mosquito

 

test chickens and rabbits act as "sentinels"

Term
What are some examples of Mosquito Vector related disease?
Definition

Viral encephalitis

Yellow Fever (Aedes aegypti)

Dengue Fever

Malara (Anopheles mosquito)

Term
Trypanosoma cruzi
Definition

phylum Mastigophria

etilogic: Chaga's disease

Vector: Reduvid (kissing) bug

-Protozoan that affects circulatory system

-can have for decades before problems occur

Term
Trypanosomoa bruceii
Definition

etiologic agent: African Sleeping Sickness

 

vector: Tsetse fly

 

-affects brain, causes coma, then death

Term
Sarcoptes scabiei
Definition

Scabies - mites

Intense skin itchiness

-not the same mite that causes mange in animals

Term
Pediculosis
Definition

lice

 

Pediculus humanis - body louse

Pediculus humanis capitis - head louse

Pediculus corporus - body

Phthirus pubis - crab lice

Term
Louis Pasteur coined the term virus which means...
Definition
poison
Term
Examples of the types of viruses
Definition
Colds, Influenza, HIV, Measles, Mumps, Rubella, Chickenpox/Shingles, Smallpox, Polio, Rabies, Warts, Herpes, Mononucleosis, Hepatitis
Term
What do we do to view virusues?
Definition
Electron Microscope
Term
Can viruses infect all cells?
Definition

all types of cells appear to have viruses that can infect them

 

-each organisms has their own specific virus

 

-viruses are obligate intracellular parasites (cannont do anything w/o a living cell)

Term
What is a virion?
Definition

an individual virus particle

 

Protein capsid and DNA or RNA (never both)

Term
How do viruses replicate?
Definition
The Lytic Cycle or the Lysogenic cycle
Term
Explain the Lytic Cycle
Definition

Attachment - the phage attaches to a bacterial cell

Penetration: it penetrates the cell injecting its DNA or RNA

Biosynthesis: its DNA tells the cell what to do

-Viral components are then assembled into virions

-the host cell lyses and new virions are released

 

(Quick Kill Cycle)

Term
Explain the Lysogenic Cycle
Definition

When a virion invades the bacterial host cell, the viral genetic material becomes incorporated into the the host genetic material. It replicates and can lie dormant for generation of binary fission. Stresses to the cell can cause the prophage to remove itself from the bacterial chromosome association causing the lytic cycle to occur in the infected cell.

(Postponement of Infection)

Term
What is a latent virus?
Definition
an animal virus that persists for a long time
Term
What term replaces the term prophage when referring to animal viruses?
Definition

Provirus

 

ex: HIV

Term

The following viruses cause what type of cancer?

Epstein Barr Virus

Hepatitis B and C Virus

Human Papilloma Virus

Definition

Burkitts Lymphoma

liver cancer

cervical cancer

Term
Nuclear Membrane
Definition

Pro - None

 

Eu- Present: double membrane with pores

Term
What are prions?
Definition

proteinaceous infectious particles that cause:

 

scrapi (sheep)

Kuru (New Guinea cannibals)

Mad Cow (Bovine spongiform encephalophathy BSE

Term
What is it called when people eat meat from cows infected with Mad Cow?
Definition
vCJD - variant Creutzfeld-Jacob disease
Term
Nucleolus
Definition

Pro - No

 

Eu-present in the nucleus, assembles ribosomes

Term
DNA
Definition

Pro - Single Circular Loop

 

Eu-Linear molecules with histone proteins (double helix ladder)

Term
Asexual Reproduction
Definition

Pro - binary fission

 

Eu-mitosis

Term
Sexual Reproduction
Definition

Pro- Conjugation

 

Eu-meiosis, followed by fertilization

Term
Cell Membranes
Definition

Pro - No Sterols, except mycoplasms

 

Eu - have sterols

Term
Ribosomes
Definition

Pro - 70s

 

Eu-80s except in mitochondria and chloroplasts

Term
Membrane Bound organelles
Definition

Pro - None

 

Eu-mitochondria, chloroplast, E.R, Golgi bodies, Lysosomes

Term
Cell Respiration
Definition

Pro-in cytoplasm and cell membrane

 

Eu-cytoplasm and mitochondiria

Term
Photosynthesis
Definition

Pro-chlorophyll on folds of cell membrane

 

Eu-chlorophyll in chloroplasts

Term
Cell Walls
Definition

Pro- Peptidoglycan

 

Eu-plants-cellulose

fungi-chitin

Term
Glycocalyx
Definition

Pro - some

 

Eu- some

Term
Endospores
Definition

Pro- Bacillus and Clostridium

 

Eu-None

Term
Gas vesicles
Definition

Pro- Photosynthetic bacteria

 

Eu-none

Term
Appendages
Definition

Pro- Flagella, Pili, Fimbrae

 

Eu- Flagella, Cilia, psuedopods

Term
Microtubules
Definition

Pro - None

 

Eu- "9 and 2" arrangements in flagella and cilia

Term
Size
Definition

Pro- <10 microns

 

Eu- > 10 microns

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