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-derived systematic nomenclature and built taxonomy -based on 16S of 30S |
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-bacteria -archaea -eukarya -drives microbiology organization |
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-Purple Sulfur -photoautotroph -oxidizes sulfur and deposits it on the cell -H2S-->S(0)-->So4(-2) -found in saline lakes, soda lakes, and salterns |
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-Purple Non-Sulfur Bacteria -photoheterotroph -don't fix CO2 -ferment in dark |
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-CH3 CH2OH//O2-->CH3COOH -respires alcohol to acetic acid -fruit spoiling aerobically produces vinegar |
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Ecology and Industrial Issues |
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-found in alcoholic jouces -CaCO3 plates -incomplete oxidation of organic compounds -synthesize cellulose |
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-Gm- coccus (unusual) -Gonorrhea and Meningities |
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-rod-shaped -Violaceum--purple pigment -very resistant to antibiotics -bone/sinus infections with purple ooze -malignant -very clean H2O |
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-cholera -deep sea fish -bent rods -OXIDASE POSITIVE |
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Photobacterium and Bioluminescence |
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Definition
-marine, associated with fish -grow in special organ or in dead fish -facultative aerobes -luciferase, reduced flavin mononucleotide, and O2 required |
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-luciferase regulatory synthesis -produce autoinducer -quorum sensing |
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Regulation of Bioluminescence |
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-only when conditions are favorable for the development of high population densities |
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Rickettsias Metabolism and Pathogenesis |
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Definition
-intracellular -Rocky Mountain Spotted Fever -Epidemic typhus -oxidize glutamate or glutamine -respiratory chain with cytochromes -electron transport phosphorylation -rest of nutrients from cell -transmitted by arthropod vectors -penetrate epithelial cells of GTI, multiply, appear in feces |
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-rod-shaped, intracellular in arthropods -parthenogenesis, killing of males, and feminization (males develop into females) -colonize in insect egg |
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-nitrosifying -chemolithotrophs -utilize inorganic electron donors -ammonia oxidizing -ammonia monooxygenase |
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-chemolithotrops -nitrite-oxidizing bacteria -nitrite oxidase |
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Ecology Isolation and Culture of Nitrifying Bacteria |
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-soil and water -high ammonia content -mineral salts media |
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-rod-shaped -acidophilic -Fe+2//O2-->Fe+3 -H2S + 2O2-->SO42- + 2H+ -S(0) + H2O + 1 1/2 O2 --> SO42- + 2H+ S2O3 2- + H2O + 2O2 --> 2SO4 2- + 2H+ |
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Acidothiobacillus ferroxidans |
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-Fe+2//O2-->Fe+3 -filamentous, gliding, sulfur-oxidizing -many cells from end to end -habitats rich in H2S -Winogradsky's 1st -chemolithotropic autotrophs -rhizosphere of plants -settling problems in sewage |
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-sulfur-oxidizing -chemolithotrophic -all bundles surrounded by sheath -anoxic oxidation of H2S -accumulate huge amounts of nitrate and support extended periods of anaerobix resp w/ H2S as e- donor |
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-H2//O2-->H2O -H2S//O2-->SO4-2 -H2 sole e- donor -O2 sole e- acceptor -Knallgas reaction -more than 1 hydrogenase -facultative -hydrogen oxidizing |
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Physiology and Ecology of "H" bacteria |
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Definition
-grow best under microoxic conditions -5-10% of O2 levels -nickel in media |
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CO Oxidation (Pseudomonas and carboxydovorans) |
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Definition
-grow on CO -carboxydotrophic -Calvin cycle Rxns -Co -> CO2 by codehydrogenase -pterin bound to ^ |
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Pseudomonas carboxydovorans |
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-can grow anaerobically with natrate as e- acceptor |
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C1 Metabolism in Methanotrophys and Methylotrophs |
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-no C-C bonds -require O2 as reactant in oxygenation -obligate Carbon -methanotrophs have sterols = essential part of internal membrane system in Ch4 oxidation -methanotrophs have methane monooxygenase |
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-assimile on C via ribulose monophosphate cycle -disc shaped vesivales - lack of complete CAC - obligate methylotrophs |
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-assimilate c1 intermediate via serine pathway -paired membranes -hold metahne monooxygenase |
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-oxidize methane -Ch4 = e- donor -aerobes -soil and water -grow with one-c organic compound and methane |
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oxidize methane grow with one carbon organic compound |
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Ecology and Isolation of Methano and Methylo trophs |
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aquatic and terrestrial environments METHANE!! narrow badn at oxycline mineral salt medium 80% methane 20% air |
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Methanotrophic Symbionts of Animals |
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-relationships with mussels and sponges -hydrocarbon seeps on teh seafloor, places where methane is released -mussel gills consume methane where TYPE 1 Methanotrophs are located |
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-straight or slightly curved cemoorganotrophic aerobic rods with polar flagella -absence of gas formation from glucose -catalase positive -OXIDASE POSITIVE -0.5-1.0 micrometer -no spores -no sheaths -never fermentative -H2 or CO sole e- donor -nitrate e- acceptor anaerobically -responsible for breakdown in oxic habitats - |
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-6-phosphateglyconate dehydrase -2-keto-3-deoxyglucosephosphate aldolase -not in Gm+ |
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-Fermentation of sugars to ethanol -Spittle Beer -Entner-Doudoroff pathway -fermentation of agave -yogurts, cheese -homofermentive |
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-fastest rate of respiration -N2 requires two reducing equivalents (ATP, Fd) because no living plant/animal can fix pure N -Slime layers impede 02 -can decouple ETS to consume tremendous amount of oxygen |
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-not pathogenic except Bdellovibrio |
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-prey on other Gm- bacteria -develops intraperiplsmically -froms a spehrical structure called a bdelloplast -oxidation of amino acids and acetate |
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-magnetotaxis -orient long axis with northsouth magnetic field -magnetosomes |
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-microaerophile -bacterial diarrhea -contaminates alomst 100% of poultry carcass -most common cause of undiagnosed bacterial diarrhea |
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-acute and chronic gastritis -fromation fo peptic ulcers -related to campylobacter |
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-oxidize Fe+3 -clogs pipes because filaments tangle -sheath -filament is a chain of rod-shaped cells -obligate aerobe |
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-SEWAGE FUNGUS -precipitates Manganese -sheath |
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-inside H2O treatment cartidges' -like the Carbon -cytoplasm filled stalk -chemoorganotroph -holdfast structure attaches to surface |
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-twisted stalklike structure of iron -bean shaped cells -cannot be grown successfully -might represent lithotrophy -in neutral pH iron oxidation produces even smaller exergonic reactions |
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-largest genomes of any prokaryote -fruiting body formation -can form multicellular structures -have reproduction |
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-Oxidase "-" -Catalase "+" -facultative aerobe -forms cell clusters -commensals and parasites of humans and animals |
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-devil bugs that fall on plate -found in air -citochrome C oxidase in ETS -Oxidase "-" Catalase "_" -shows up as pure white or pink -w/stand drying well -obligate aerobe -acid from glucose aerobically -no cell clusters |
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-no respiratory activity -heterofermental -EM Pathway -buttermilk, cheese, medical, etc. -produce lactic acid |
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-homofermenter -pyruvate --> lactic acid -antimicrobial properties -Bulgarian buttermilk -rod-shape |
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-make lactic acid -plagues food industry (listeriosis) -largest meat recall in '93 -likes fridge -80-85% spontaneous abortion--crosses placental barrier |
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-few are bad one causes eye infections -form endospores -harmless unless anthracis -thurigienesis - bad for butterflies -protoxin converted to toxin by prteolyic cleavage in the larval guy -binds to intstinal epithelial cells and induces pore formation -can clone into crops |
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-found in soils -makes endospores -food poisoning--BOTULISM --most potent biological poison -1mg = >1 million rats dead -tetanus -pyruvate-->solvents -ATP throuh substrate level phosphoryl. -sugars--butyric acid |
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-no cell wall -wormy -subject to osmotic shock -obligate -small genome - 500-1100 kbp -steals steroids for their membranes -M. genitalium |
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-parasitic to sucking insectes -contain genes that appear to come from plants/insects -helical or spiral shaped -rotary motion makes them motile |
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-arthrobacter -weird shapes V-shaped -diptheriae |
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-Swiss Cheese! -propionic acid -thought fermenter -membrane has a sodium translocating ATPase to pump sodium out |
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Mycobacterium Ziehl-Neelsen Stain |
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-fuchsin and phenol -steam for 2-3 min to force dye into cell -acid-fast = red -non = blue -mycolic acid necessary for acid fastness |
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-pleomorphic -slow growers and fast growers -TB = slow -tight, compact, wrinkled colonies -simple mineral salt media -form yellow carotenoid pigments -classifications: nonpigmented, forming pigment in light, pigment in dark |
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-+500 species -most clincal antibiotics -found in soil -excrete substances--poisons -filaments are .5-1 micromete diamter, infinite length -growth occurs at tips of filaments -spores are called conidia -conidia produced through formation of cross-walls |
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Ecology and Isolation of Streptomyces |
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-primarily soil -odor of soil from production of geosmins -alkaline and neutral soil favorable -well-drained soils |
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Antibiotics of Streptomyces |
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-50% proved to be antibiotic producers -500 antibiotic substances produced -streptomycin, spectionmycin, neomycin |
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-old name blue-green algae -oxygenic photosyntheseis -Z scheme -chlorophyll A -phycobilins -heterocyst (round, seemingly empty cells, along a filament or an end of one) |
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-refractile like a diamond -heterocyst fixes ntirogen (azoto) -can blow up cell w/ a lot of O2 -no photosystem 2 -block O2 diffusion bcuz refractile |
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-can fix nitrogen in dark -danger of O22 eliminated -nitrogenase closes in light -changes quarternary structure to close in light |
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-part of endosymbiotic theory -first procholorophyte discovered -marine invertebrates -muramic acid --> peptidoglycan present -carotenoids similar to cyanobacteria |
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-Champion STD -elementary body attacks host cell -phagocytosis of elementary body -conversion to reticulate body -multiplication of reticulate bodies -conversion to elementary boides -release of elementary bodies |
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-lack peptidoglycan ***cells walls are S-layer*** -large amounts of cystine and proline -resitant to penicillin nad cephalosporin - |
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-nuceloid surrounded by nculear envelope -remains in a covalently closed, circular, and supercoiled form -remains partitioned from remaingin cytoplasm by true unit membrane |
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-oligotrophic--very little organic C and other energy sources -form cytoplasmic appendages called prostheca -two to several prostheca -divide symmetrically -peptidoglycan |
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-form prostheca -two genes that show homology to tubulins of euk. -similar to FtsZ |
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-anaerobes -prevalent in human guy -10^10/gm in human feces -proteolitic -produce abdominal infections -synthesize shingolipids |
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-aerobic -pigmented rods -F. meningosepticum = infant meningitis -cultured on agar with cellulose fibers -obligate aerobe -fish pathogens |
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-gliding motility, but no flagella -look like they crawl -can eat cellulose, peptidoglycan - |
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-Green Sulfur Bacteria -anoxygenic phototroph -S compounds e- donor -chlorophyll acde -chlorosomes (segregated structures on reaction center) -sulfur resides outside cell -consortium - associaton w/ chemoorganotrophic bacteria |
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-Gm-, motile, tightly coiled -protoplasmic cylinder -endoflagella (inside membrane) -Skinny -not spirella |
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-endoflagella -both rotate is same direction -torsion on cell |
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-Spirochete -Syphilis -never grown in vitro -oral cavities -hindgut of termites |
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-Spirochete -Lyme disease -tick-borne -some protozoa use spiros as flagella -animal or human pathogens -relapsing fever -trasmitted through insect vector -death in 40% infected |
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-15,000 Grays of radiation -red or pink due to carotenoids- -resistant to mutagenic effects due to DNA arrangement -toroidal ring -only effected by nirtosuguanidine -efficeint in DNA repair |
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-green nonsulfur -hybrid phototroph -thermophilic -lack peptidoglycan -earliest known phototrophic bacterium |
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Physiology of Chloroflexus |
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Definition
-resemble purple nonsulfur in photoautotrophy -grows well in dark by aerobic respiration as chemootrangotroph -hydroxyproponate pathway |
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-composed of species that specialize in anaerobic energy metabolism -extremely versatile at anaerobic respiration using a variety of electron acceptors, including the metals Fe3+ and Mn4+ -barrophiles and thermophiles -associated with mineral deposits |
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-chemotrophic member -nothing to do with glycerol -form bilayers -hyperthermophile -aerobic -membrane lipids contain 1,2 dialcohols -neither ester nort ether linkages -lack peptidoglycan |
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-thermophilic, sulfate reducing bacterium -growth temperature optimum is only 70 degrees Celsius -ether-linked lipids |
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