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Microbiology
Lab - Enterotube
9
Biology
Undergraduate 1
12/03/2007

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Cards

Term
What are the basic parts of the Enterotube II system?
Definition
  • 12 different media
  • Self-contained sterile inoculating wire
Term
What is the purpose of the Enterotube?
Definition
To rapidly identify enterics from a single colony.
Term
What type of bacteria does the enterotube identify?
Definition

GM-, aerobic or facultative anaerobic, bacillus

Term
What is required to use the Enterotube?
Definition
  • Isoloted colony on an agar plate that is selective for GM-
  • MacConkeys, EMB, Hektoen Enteric
Term
What significant procedure needs to be followed that can effect the results?
Definition
The last 8 compartments needs holes punched in them by the sterile inoculating wire after it is broken off.
Term

If these holes are not punched how will the effect the results?

Definition

Aerobic bacteria require

Term
What is the result of the enterotube?
Definition
A five digit code that can be referenced to identify the exact genus and species name of the enteric.
Term
What are some reasons for the failure of the enterotube?
Definition
  • Contamination
  • Not a GM- colony
  • Holes not punched
Term
What would happen if you used the enterotube on a GM+ rod or cocci?
Definition
  • Most GM+ the catalase test is a primary major determinant in gram positive species and is absent from the entertube.
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