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Microbiology
Microbial Genetics Part 1
45
Biology
Undergraduate 3
11/14/2011

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Term
____________ were once considered a better candidate as the molecule to store genetic information for the cell
Definition
Proteins
Term
Proteins have ____ number of different amino acids.
Definition
20
Term
DNA possesses ______ different nucleotides.
Definition
4
Term
Why was DNA originally not considered for storing genetic information?
Definition
Thought it was too simple
Term
__________________ in _______ showed bacteria could be changed genetically by a “transforming principle”
Definition
Griffith, 1928
Term
Griffith in 1928 showed bacteria could be changed genetically by a ____________________.
Definition
transforming principle
Term
In 1944, ________, _________, and ____________ showed that the transforming principle is the chemical DNA
Definition
Avery, MacLeod, and McCarty
Term
In _________, Avery, MacLeod, and McCarty showed that the transforming principle is the chemical DNA
Definition
1944
Term
___________ and __________ in 1952 used an E. coli virus (phage) to confirm that DNA is the genetic material
Definition
Hershey and Chase
Term
Hershey and Chase in _______ used an E. coli virus (phage) to confirm that DNA is the genetic material
Definition
1952
Term
Hershey and Chase in 1952 used an _____________ virus (phage) to confirm that DNA is the genetic material
Definition
E. coli
Term
Change of non-virulent organisms into virulent ones is a result of ''_______________''
Definition
''transformation''
Term
''transformation''
Definition
Change of non-virulent organisms into virulent ones is a result of “transformation”
Term
3 Major macromolecules
Definition
1.) deoxyribnucleic acid (DNA)
2.) ribonucleic acid (RNA)
3.) proteins and enzymese
Term
What is DNA's main function?
Definition
storage molecule for genetic instructions to carry out metabolism and reproduction
Term
Wha is RNA's main function?
Definition
expresses the information in DNA
Term
What is proteins and enzymes main function?
Definition
build cellular structures and do cellular work
Term
Whos job is it to build cellular structures and do cellular work?
Definition
Proteins and enzymes
Term
Who expresses the information in DNA?
Definition
ribonucleic acid (RNA)
Term
Who is a storage molecule for genetic instructions to carry out metabolism and reproduction?
Definition
DNA
Term
What does DNA stand for?
Definition
deoxyribonucleic
Term
What does RNA stand for?
Definition
ribonucleic acid
Term
What does the term genome mean?
Definition
all DNA present in a cell or virus
Term
What does the term genotype mean?
Definition
specific set of genes an organism possesses
Term
What does the term phenotype mean?
Definition
collection of observable characteristics
Term
DNA is a polymer of _______________ _______________.
Definition
repeated nucleotides
Term
What does a nucleotide consist of?
Definition
a nitrogenous base, deoxyribose sugar, and 1-3 phosphate group(s)
Term
What are the 4 nitrogenous bases?
Definition
A, G, C, and T
Term
The 4 nitrogenous bases
(A, G, C, and T) are
grouped by structure into
__________ and ___________.
Definition
purines and pyrimidines
Term
Purines
Definition
double ring;
Adenine, Guanine
Term
Pyrmidines
Definition
single ring;
Thymine, Cytosine
Term
What is the DNA double helix held together by?
Definition
hydrogen bases
Term
DNA backbone is made of _________________ and _________________.
Definition
deoxyribose sugar and phosphate
Term
A bonds to ___________
Definition
T
Term
C bonds to _________
Definition
G
Term
G bonds to _____________
Definition
C
Term
T bonds to __________
Definition
A
Term
Each single strand acts as a template for the new “____________” strand
Definition
daughter
Term
Chargaff’s rule
Definition
complimentary pairing rule(a=t,c=g)
Term
Chargaff’s rule
Definition
complimentary pairing rule(a=t,c=g)
Term
DNA polymerases do what?
Definition
catalyze DNA synthesis
Term
DNA replication is ____________-___________.
Definition
semi-conservative
Term
Semi-conservative mean....
Definition
one parent strand and one daughter strand
Term
DNA in most Bacteria is ________________
Definition
circular
Term
______________ _________________ is where DNA is unwound & replicated
Definition
replication fork
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