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Microbiology Test 1
Engelkirk, Ch 1-5
74
Microbiology
Undergraduate 1
02/01/2012

Additional Microbiology Flashcards

 


 

Cards

Term
microbiology
Definition
study of microscopic forms of life
Term
pathogen
Definition
disease causing microorganisms
Term
nonpathogen
Definition
microbes that do not cause disease
Term
opportunistic pathogen
Definition
microbes that do not cause disease under ordinary conditions, but have the potential to cause disease should the opportunity present itself
Term
acellular microbes
Definition
microbe not composed of cells
aka: infectious particles
Ex: prions and viruses
Term
cellular microbes
Definition
microbe composed of cells
Ex: procaryotes- archaea, bacteria
eucaryotes- algae, fungi, protozoa
Term
Reasons why microbes are important:
Definition

-produce oxygen

-involved in decomposition of dead organisms and the waste products of living organisms

-decomposing industrial wastes

-involved in elemental cycles

-important links in food chains

-live in intestinal tracts ofanimals where they aid in the digestion of food, and produce substances that are valuable to host animal

-are essential in various food and beverage industries

-used to produce certain enzymes and chemicals

-as a source of antibiotics

-essential in field of genetic engineering

-used as "cell models"

-cause infectious diseases and microbial intoxications

 

Term
infectious disease
Definition

-a pathogen colonizes a person's body

-pathogen causes disease

Term
microbial intoxication
Definition

-a pathogen produces a toxin in vitro (outside the body)

-a person ingests the toxin

-toxin causes the disease

Term
Difference in infectious disease and microbial intoxication
Definition

Infectious diseases result when a pathogen inhabits the body and subsequently causes disease.

Micorbial intoxications result when a person ingests a toxin (poisonous substance) that has been produced by a pathogen outside the body. 

Term
explain the relationship between microbes and infectous diseases
Definition
microbes cause infectous diseases
Term
Leeuwenhoek
Definition

-first person to see live bacteria and protozoa

-"Father of Microbiology" (or Protozoology)

- single-lens microscopes or simple microscopes

Term
Pasteur
Definition

-discovered the process of alcoholic fermentation

-discovered forms of life that could exist in the absence of oxygen (anaerobes)

-developed a process to kill microbes that were causing wine to spoil- pasteurization

-made significant contributions to the germ theory of disease

- discovered infectious agents that caused silkworm diseases and how to prevent them

-changes in hospital practices to minimize the spread of disease by pathogens

-developed vaccines to prevent chicken cholera, anthrax, and swine erysipelas

-developed vaccine to prevent rabies in dogs and treat human rabies

Term
Koch
Definition

-Koch's Postulates

-contributions to the scientific theory of disease

-discovered B anthracis produces spores capable of resisting adverse conditions of anthrax

-developed methods of fixing, staining, and photographing bacteria

-developed methods of cultivating bacteria on a solid media, thus obtaining pure cultures

-discovered the bacterium that causes tuberculosis and the one that causes cholera

-lead to a skin test valuable in diagnosing tuberculosis

Term
abiogenesis vs biogenesis
Definition

abiogenesis is the idea that life can arise spontaneously from nonliving material.

 Biogenesis is the theory that life can only arise from preexisting life.

Term
germ theory of disease
Definition
Term
Koch's Postulates
Definition
1. the microorganism must always be found in similarly diseased animals but not in healthy ones
2. The microorganism must be isolated from a diseased animal and grown in pure culture.
3. The isolated microorganism must cause the original disease when inoculated into a susceptible animal
4. The microorganism can be reisolated from the experimentally infected animal
Term
exceptions to Koch's Postulates
Definition
Term
simple microscope
Definition
microscope containing only one lens
3-20x mag.
Term
compound light microscope
Definition
microscope with more than one lens that uses visible light as source of illumination
-ocular x objective to get magnification
-oil-immersion= 1000x
Term
electron microscope
Definition
-use electron beam as source of illumination and magnets to ficus the beam
-have greater resolving power than CLM
-can NOT be used to observe living organisms; they are killed during the specimen-processing procedures
-both types have built in camera systems
Term
types of electron microscopes
Definition
-Transmission electron microscope- has tall column at the top of which an electron gun fires a beam of electrons downwards; .2nm resolving power; 1 million x mag.
-Scanning electron microscope- shorter column and specimen is placed at bottom of column; used to observe outer surfaces of specimens; 20 nm resolving power
Term
Atomic Force microscope (AFM)
Definition
enables scientists to observe living cells at extremely high mag. and resolution under physiological conditions
-provides a true 3D surface profile
Term
types of Compound light microscopes
Definition
-brightfield- object against a brigh background
-darkfield- object is illuminated against a dark background
-phase-contrast microscope- observe unstained living microorganisms
-fluorescence microscope- built in UV light source, emits longer wavelength of light, causing object to glow against dark background (type of immunodiagnostic procedure)
Term
cell theory
Definition
scientific conclusion that all plant and animal tissues are composed of cells
Term
cell membrane function
Definition
-encloses and holds cell intact, separating contents of cell from outside world ("skin")
-allows only certain substances to pass through it, regulating the passage of nutrients, waste products, and secretions into and out of cell= selective permeability
-aka plasma mem or cytoplasmic mem
Term
nucleus function
Definition
-controls functions of entire cell
-"command center of cell"
Term
ribosome function
Definition
-site of protein synthesis
Term
Golgi complex function
Definition
-connects or communicates with ER
-completes the transformation of newly synthesized proteins into mature, functional ones and packages them into small, membrane-enclosed vesicles for storage w/in the cell or export outside the cell (exocytosis or secretion)
-"packaging plant"
Term
lysosome function
Definition
-contain lysozyme and other digestive enzymes that break down foreign material taken into the cell by phagocytosis
-aid in breaking down worn out parts of the cell and may destroy the entire cell if the cell is damaged or deteriorating (autolysis)
Term
mitochondrial function
Definition
- where most ATP molecules are formed by cellular respiration
-"power plant of cell"
Term
plastid function
Definition
-site of photosynthesis (conversion of light energy into chemical energy)
Term
cytoskeleton function
Definition
-strengthens, supports, and stiffens the cell to give it its shape
Term
Eucaryotic cell wall function
Definition
provides rigidity, shape, and protection for cell
Term
flagella
Definition
-the whipping motion of flagella allows cells to swim through liquid environments
-organelle of locomotion
Term
cilia
Definition
-organelle of locomotion
-beat with a coordinated, rhythmic movement
Term
chromosome function
Definition
-control center of bacterial cell
-can duplicate itself, guide cell division, and direct cellular activities
Term
Procaryotic cell wall function
Definition
-same function as euk; provide rigidity, shape and protection
-is much more chemically complex than euk
Term
capsule function
Definition
-serve an antiphagocytic function, protecting the encapsulated bacteria from being ingested (phagocytized) by phagcytic white blood cells
Term
Procaryotic flagella function
Definition
-enable bacteria to move
-number and arrangement can be used for classification and identification purposes
-bacteria possess flagella, never cilia
Term
pili function
Definition
-not associated w/ motility; organelles of attachment
-one type enables bacteria to adhere or attach to surfaces
-other type enables transfer of genetic material from one bacterial cell to another following attachment of cells to each other
Term
endospore function
Definition
-enable bacteria to survive adverse conditions, such as temp extremes, dessication, and lack of nutrients
Term
genus
Definition
first part in name of a species
Term
specific epithet
Definition
2nd part in name of a species
-cannot be used alone
Term
species
Definition
specific member of a given genus
Term
Hooke
Definition

-first person to use the term "cells"

-small, empty chambers in the structure of cork= cell

Term
Schleiden and Schwann
Definition
-concluded that all plant and animal tissues were composed of cells= cell theory
Term
Virchow
Definition
-proposed the theory of biogenesis= that life can arise only from preexisting life, and cells only from preexisting cells
Term
characteristics used to characterize viruses
Definition

1. type of genetic material (DNA vs RNA)

2. shape of capsid

3. number of capsomeres

4. size of capsid

5. presence or absence of an envelope

6. type of host that it infects

7. type of disease it produces

8. target cell

9. immunologic or antigenic properties

Term
5 properties that distinguish viruses from bacteria (or living cells)
Definition

1. have either DNA or RNA, not both like living cells

2. unable to replicate on their own, must invade a host cell

3. do not divide by binary fission, mitosis, or meiosis

4. lack the genes and enzymes necessary for energy production

5. depend on ribosomes, enzymes, and metabolites of host cell for protein and nucleic acid production

Term
3 viral diseases in humans
Definition

-HIV

-meningitis

-measles

-mumps

-influenza

 

Term
viroid vs virion
Definition

viroid- infectious RNA molecule

-cause plant diseases


virion- complete viral particle

-can infect humans, animals, plants, fungi, protozoa, algae, and bacterial cells

Term
ways to classify bacteria
Definition

1. gram-negative

2. gram-positive

3. those that lack a cell wall

or

-can classify on the basis of their relationship to oxygen and CO2

Term
purposes of fixation
Definition

1. kills the organisms

2. preserves their morphology (shape)

3. anchors smear to the slide

Term
diplococci
Definition
pairs of cocci
Term
streptococci
Definition
chains of cocci
Term
staphylococci
Definition
clusters of cocci
Term
octads
Definition
packets of 8 cocci
Term
tetrads
Definition
packets of 4 cocci
Term
coccobacilli
Definition
elongated bacilli (rods)
Term
diplobacilli
Definition
pairs of bacilli
Term
streptobacilli
Definition
chains of bacilli
Term
pleomorphism
Definition
bacterial species with the ability to exist in a variety of shapes
Term
obligate aerobe
Definition
require an atmosphere containing about 20% oxygen to grow and multiply
Term
microaerophiles
Definition
require reduced oxygen concentrations (about 5%)
Term
obligate anaerobe
Definition
can only grow in an aerobic environment (no oxygen)
Term
aerotolerant anaerobe
Definition
-does not require oxygen -grows better in the absence of oxygen -can survive in atmospheres containing oxygen
Term
facultative anaerobe
Definition
-capable of surviving in either the presence or absence of oxygen
Term
capnophiles
Definition
grow better in the lab with an atmosphere containing 5%-10% CO2
Term
bacterial diseases in humans
Definition

-Pneumonia

-nongonococcal urethritis (NGU)

-Rocky Mountain Spotted Fever

Term
how archaea are diff from bacteria
Definition

-more closely related to procaryotes

-many are extremeophiles (live in extreme environments)

-cell walls have no peptidoglycan

-diff in rRNA structure

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