Term
|
Definition
Coccus (Circular)
Bacillus (Rod Shape)
Spirillum (Wormy) |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Grape-like clusters of cocci |
|
|
Term
|
Definition
|
|
Term
|
Definition
| 2 tetrads, forming a cube |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| Spore-containing, looks like a club. |
|
|
Term
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| The transferring of genes as naked DNA in a solution. |
|
|
Term
|
Definition
| The combining of DNA from two different cells resulting in a recombinant. |
|
|
Term
|
Definition
| The use of DNA to make RNA to make proteins. |
|
|
Term
| Which bacteria can undergo transformation naturally? |
|
Definition
E. coli
Streptococcus
Acinetobacter
Bacillus
Haemophilus
Neisseria
Staphylococcus |
|
|
Term
| Factors affecting transformation |
|
Definition
|
|
Term
|
Definition
|
|
Term
|
Definition
| The antibiotic, Ampicillin |
|
|
Term
|
Definition
|
|
Term
| What turns on the Green Fluorescent Protein in the LB agar plate with +pglo? |
|
Definition
|
|
Term
|
Definition
| Passing a slide through a flame, killing the bacteria cells and adhering them to the slide to prevent them from washing off during staining. |
|
|
Term
|
Definition
Process of using only one stain.
Can only tell cell shape and arrangement. |
|
|
Term
| What did Christian Gram discover in 1883? |
|
Definition
| bacteria fall into two groups based on the stain they retain. |
|
|
Term
| Gram Stain Staining Process |
|
Definition
Primary Stain (Crystal Violet): apply for 1 minute
Mordant (Gram's Iodine): apply for 1 minute
Decolorizer (Acetone or Ethyl Alcohol): apply for 2-10 seconds
Coutnerstain (Safranin Red): apply for 30 seconds-1 minute |
|
|
Term
|
Definition
Stain purple.
Retain Crystal Violet (primary stain). |
|
|
Term
|
Definition
Stain red.
Retain Safranin Red (counterstain).
Must be decolorized before counterstain. |
|
|
Term
| Things that can cause false/inaccurate Gram Stain results include |
|
Definition
A gram-positive culture older than 24hrs will appear gram negative.
If a smear is too thick, proper decolorization may not be possible.
Over-decolorization or under-decoloration.
If safranin is left on slide too long, it will stain over any purple present, making gram-positive bacteria appear gram-negative. |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Structure that keeps bacteria alive during adverse conditions. |
|
|
Term
| What are spores resistant to? |
|
Definition
| Boiling, UV light, sun light, drying, disinfectants. |
|
|
Term
|
Definition
Auto-clave
Dry Heat
Sporicidal Disinfectant |
|
|
Term
|
Definition
Central (Middle of Cell)
Subterminal (Near end of Cell)
Terminal (At end of Cell) |
|
|
Term
|
Definition
Used to identify bacteria that cause TB.
Used to stain bacteria with a high was content in the cell wall. |
|
|
Term
| Acid-Fast Staining Procedure |
|
Definition
Primary (Carbolfuchsin [Red])
Decolorizer (Acid Alcohol)
Counterstain (Methylene Blue) |
|
|
Term
|
Definition
Mycobacterium Tuberculosis
Nocardia |
|
|
Term
|
Definition
| Retain the primary stain and appear as little red beads or little red rods |
|
|
Term
|
Definition
| Retain the counterstain and have a blue field of view |
|
|
Term
|
Definition
Streptococcus Pneumoniae (leading cause of pneumonia)
Klebsiella Pneumoniae (causes pneumonia, wound infections, and UTIs)
Cryptococcus Neoformans (yeast that produces capsules) |
|
|
Term
|
Definition
|
|
Term
|
Definition
| Have one flagella one each side |
|
|
Term
|
Definition
| Have 2+ bacteria on one side |
|
|
Term
|
Definition
| Have flagella all over the surface |
|
|
