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Microbiology Lecture 15 Vocab
mRNA Translation to Protein
20
Biology
Undergraduate 2
02/22/2009

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Term
Intron/Exon
Definition

Intron: Section of mRNA that is cut OUT of the mRNA sequence

 

Exon: Section of mRNA that is left IN the mRNA sequence

Term
Spliceosome
Definition
Recognizes the junction between introns and exons, binds to two junctions and removes the intron as a loop
Term
Ribosome
Definition
Protein unit that is composed of a small and large subunit to read mRNA and create an AA chain
Term
A site/P site/E site
Definition

A: Where the tRNA with the amino acid enters the ribosome

 

P: Where the amino acid is added to the amino acid chain

 

E: Where the empty tRNA goes to exit the ribosome

Term
Small Subunit/Large Subunit
Definition
Two components of the RNA that leaves a hole in between the two that allows the mRNA to pass through
Term
Codon
Definition

Group of three nucleotides

 

This is used to read mRNA (in groups of three)

Term
Reading Frame
Definition

The three ways to read a long line of nucleotides in an mRNA sequence

 

Needs to read the right way in order to code for the right protein

Term
Shine-Dalgarno Sequence
Definition
The specific RNA sequence that signals where the ribosome binds
Term
tRNA
Definition
 A type of RNA that has an anti codon sequence to match the codon sequence in the mRNA and the amino acid that the codon codes for
Term
Anticodon Loop
Definition

Contains the complementory sequence that goes with the codon in the mRNA

 

One end of tRNA

Term
Charged tRNA
Definition
tRNA that contains an amino acid on it
Term
Amino-acyl-tRNA synthetase
Definition
The enzyme that binds the AA to the tRNA
Term
Initiation Complex
Definition

When the small and large subunits combine in the ribosome, moves until it finds AUG in the P site

 

tRNA recognizes AUG

 

The P site is full and the A and E sites are empty

Term
Elongation
Definition

The process of continually adding

 

Costs 3 GTP per AA

 

AA in P site is transferred to AA in A site (transpeptidation)

 

P site empty tRNA exits

 

mRNA slides along to next codon in A site (translocation)

Term
Transpeptidation (peptidyl transferase)
Definition
When AA in P site binds to AA in A site
Term
Translocation
Definition

mRNA movement

 

mRNA slides to next codon in A site

Term
Stop Codon
Definition

Terminates the elongation process

 

No tRNA for stop codon

 

 Ribosome falls apart and releases the protein

Term
Release factor
Definition

Instead of a tRNA binding to the stop codon this binds to the codon

 

Ribosome falls apart and releases the protein

 

 

Term
Read Through
Definition

Sometimes a tRNA DOES recognize a stop codon

 

However it's ok because the sequence beyond the stop codon is random and there is 3/64 chance another stop codon will stop elongation

Term
"Coupled" Transcription and Translation
Definition
Only in prokaryotes where mRNA can be elongated before it is even done being transcribed into mRNA
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