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Microbiology Lecture 14 Vocab
DNA Replication and Transcription to mRNA
35
Biology
Undergraduate 2
02/21/2009

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Term
Central Dogma
Definition

The process of DNA making RNA (mRNA) which makes protein

 

 

Term
Replication
Definition

When DNA makes another copy of itself

 

Duplicates original molecule

Term
Transcription
Definition
Process of making RNA (mRNA) from DNA
Term
Reverse Transcription
Definition
When some kinds of RNA can go back and make DNA
Term
Translation
Definition
When protein is made from RNA (mRNA)
Term
Antiparallel Double Helix
Definition

When the 5' end of one strand pairs up with the 3' end of the other strand of DNA

 

One strand is "flipped upside down"

Term
Hydrogen Bonded Base Pair
Definition

When the nitrogenase bases pair up with one another in DNA or RNA with hydrogen bonds

 

A goes with T (or U in RNA)

 

C goes with G

Term
Phosphodiester Bond
Definition

In DNA

 

Bonds the phosphate group and five carbon sugar together

Term
5' Phosphate?
Definition
Phosphate group is attached to 5th carbon of the sugar in DNA
Term
3' Hydroxyl
Definition
Hydroxyl group (-OH) is attached to the 3rd carbon of the sugar in DNA
Term
Pyrophosphate?
Definition
Provides the energy that comes from splitting P-P from nucleoside triphosphate to attach the phosphate group to the sugar group
Term
Primer
Definition

A strand of nucleic acid that serves as a starting point for DNA replication

 

Where the existing 3' OH attached to existing nucleic acid polymer

Term
Template
Definition

When DNA replicates there needs to be an old strand so the new can complement the old strand

 

The old strand is referred to as the template

Term
Proofreading
Definition

A process where if a base is wrong, the H-bond is made badly, and the base is removed by DNA pol

 

The template then tries again to make a duplicate copy of itself

 

Reduces mutational errors (not for RNA)

 

Term
Semiconservative
Definition

When DNA replicates there is an old strand and a new strand that complements the old strand.

 

Its semiconservative because only one of the old strands is saved for replication

Term
Bidirectional
Definition
In bacteria where DNA gets copied in both directions pulling both ends into another circle
Term
Theta Replication
Definition
When bacteria replicate their DNA it looks like a theta symbol because it is in a circular shape to begin with
Term
Replication Bubble
Definition

When the DNA begins to unwind to begin replication forming a bubble

 

See sketch in notes?

Term
Ori C/Ter
Definition

Ori C: specific sequence that begins replication and proceeds in both directions

 

Ter: specific sequence where ends replication 180° away around the circle

Term
Leading Strand
Definition

The strand of DNA that able to replicate the DNA in a continuous fashion

 

Replicates in same direction as fork opens

 

3' to 5'

Term
Lagging Strand
Definition

Replicates in the opposite direction as fork opens

 

This strand replicates the DNA in fragments

 

5' to 3'

 

This strand has to be looped back on itself

Term
Okazaki Fragments
Definition
The short fragments of DNA made by the lagging strand in the 5' to 3' direction
Term
Helicase
Definition
Enzyme that opens up the two strands of DNA
Term
Gyrase
Definition
Enzyme that relieves the strain of replication of DNA when it gets wrapped up again
Term
Primase?
Definition

Enzyme that makes sure that each new piece of DNA is preceeded by a primer

 

Makes the primer?

Term
DNA polymerase
Definition

Enzyme that makes the phosphodiester bonds

 

enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand

Term
RNA polymerase
Definition
Enzyme that makes RNA
Term
Sigma factor (sigma subunit)
Definition
One part of the RNA polymerase that allows it to find the promoter on the DNA
Term
Core Enzyme
Definition
Enzyme part of RNA polymerase that makes the RNA
Term
Promoter
Definition
A special DNA sequence that RNA polymerase tries to find in order to make RNA
Term
Consensus Sequence
Definition
Two particular sequences of DNA that indicate where the -35 and -10 positions are on the DNA for transcription
Term
-10/-35 sites
Definition

Lets the RNA polymerase know if it is going in the right direction along the DNA strand

 

Going from -35 to -10 is the right direction

Term
Gene
Definition
A basic unit of heredity of in a living organism
Term
Template strand/ Non template strand
Definition

The direction of transcription determines dictates which strand is used as a template

 

Template: Used to make RNA

 

Nontemplate: Not used make RNA

Term
Hairpin Loop Terminator
Definition
The stop signal that indicates the end of the gene in DNA
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