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Definition
Microscope occular.
Eye peice (usually 10x magnification) |
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Microscope objective
we use 100x for oil immersion |
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Definition
Microscope condensor
directs light towards the objective lens in bright feild micsopcopy
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Definition
resolving power.
expressed as d (the smallest distance between two objects that can be seen as seperate) small d= better resolution
d=λ/2NA |
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Definition
Diplococci
divide in one plane |
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Definition
Steptococci
Divide in one plane |
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Definition
Tetracocci (tetreads) Divide in two planes |
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Definition
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Definition
Sarcinae
divide in three planes |
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Term
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Definition
basic, positively charged chromophore
Ex: Methylene Blue and Crystal Violet |
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Definition
acidic, negativley charged chromophore
EX: Acid Fuchsin, Congo Red, Nigrosin |
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Term
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Definition
no charge
Ex: Sudan black
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Term
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Definition
Water insoluble
EX: India ink |
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Definition
Negative stain
size and shape
stains the background, not the cell.
(NOT DARK FEILD MICROSCOPY)
two dyes used:
-Nigrosin (negative so reppeled by neg charge on bacteria)
-India ink (insuluble so doe snot penetrate cell) |
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Term
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Definition
-put the dots of bacteria and dye then spread with another slide at 45 degree angle
DO NOT RINSE OR HEAT FIX |
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Term
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Definition
Simple stain
shape
one dye used to stain all cells the same color
Cationic dyes (methelyne blue and crystal violet) |
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Term
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Definition
Gram stain
Differential: based on cell wall
Dye: Crystal violet
Mordant: Iodine
Decolorizer: Ethanol
Counter stain: Safranin
Gram+ Purple
Gram- pink |
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Term
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Definition
Acid fast stain
Differential: based on cell wall (Mycolic wax content)
Primary stain: Carbol Fuchsin
STEAM
Decolorizer: Acid alcohol
Counterstain: Methylene Blue (cationic)
(High wax) ACID FAST+ RED
ACID FAST- BLUE |
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Term
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Definition
Spore stain
structural (specialized)
Primary stain: Malachite Green
STEAM
Decolorizer: water
Counterstain: Safranin
Endospore: green in center of pink
sporangium: pink
free spore: green oval bodies
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Term
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Definition
AGAR:
Broth: liquid
Slant: Solid
Deep: solid |
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Term
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Definition
Phenylethyl Alcohol Agar
Selects for growth of gram+ |
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Term
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Definition
Desoxycholate Agar DES
selective and differential
selects for growth of gram-
red colony: lactose+
nonred colony: Lactose-
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Term
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Definition
Eosin Methylene Blue (EMB) SELECTIVE AND DIFFERENTIAL
selects for gram-
Differentiation:
Dark colony: lactose ferm & produce alot of acid
pink colony: lacrtos ferm
no color absorbed: lact- |
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Term
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Definition
Blood agar plate
differentiates based on reactions to blood
"BAG"
A: Beta hemolysis
b: Alpha hemolysis
c: Gamma hemolysis
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Term
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Definition
tests for amylase
Indicator: iodine (added after growth)
colorless around colony: +
purple around colony: -
(because iodine+starch=purple so this means th amylase was not there to hydrolize the starch) |
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Term
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Definition
tests for caseinase (Hydrolizes casein-Milk)
A: -
B: + |
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Term
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Definition
tests for lipase
Blue dye intensifies: + (lower pH)
no blue intensification:- |
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Term
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Definition
Phenol Sugar Fermentation and Durham tube
(the first tube shows the control)
yellow: ferm+
lt orange-red:ferm-
Gas in tube: Gas prodctuction |
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Term
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Definition
Methyl Red
tests for mixed acid fermentors
indicator: Methly red (after growth)
dye stays red: + pH under 5.1
dye turns yellow: - |
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Term
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Definition
VP
test for 2,3 butanediol fermenter
Reagents=
Barrit's reagents.
VP1: Alpha-nahthol
VP2: KOH
red on top:+
no red:- |
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Term
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Definition
Catalase
differential
detects catalase
bubbles:+
no bubbles:-
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Definition
Oxidase
differential: detects oxidase whih removes hydrogen
aromatic ameine used
(oxidase reagents added after growth)
colorless:-
dark purple/red to dark blue:+ |
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Definition
Nitrate broth
Differentail: tests for nitrate reductase
red w/o zinc: +
red w/ zinc: -
Never red: + |
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Definition
Tryptone
differential tests for indole (to see if tryptonase is present because it turns tryptone into indole )
Indicator: Kovac's reagent
red top: indole+ so tryptonase +
yellow top: indole- so tryptonase -
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Term
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Definition
Urea
Differential
detects urease
indicator: phenol red
Red to cerise=+
yellow to orange=- |
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Term
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Definition
SIM
Sulfur Indole Motility
H2S positive: Black
Indole+: Kovac's reagent turns red after addition
Motility+: growth away from stab line |
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Definition
Simmons citrate slant
differential
detects for the use of citrate as sole carbon source
starts green
No growth:- (reagardless of color change)
Growth and Blue color: + |
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Term
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Definition
differential
tests for presence of phenylalanase
indicator: ferric chloride added after growth
green: +
no color change to ferric chloride: -
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Definition
Gelatin
detects gelitinase
resolidification: -
no resolidification: + |
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Definition
OF GLUCOSE
Tests to determine if a bacteria can use glucose in an oxidative (aerobic) or fermentative (anerobic) condition.
On open tube and one closed (added oil)
Results:
-OPEN CLOSED: Incompletly Oxidative (O)
-OPEN CLOSED: Strictly Fermentation (F)
-OPEN CLOSED: Strictly Oxidative
-OPEN CLOSED: Faculative
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Definition
KIA tests for the ability to ferment glucose and/or lactose, tests H2S production and can be used to test for gas production.
Results:
>Red slant/Red butt: Gluc- Lac- H2S-
>Red slant/ yellow butt: Gluc+ Lac- H2S-
>Yellow slant/ Yellow butt: Gluc+ Lac+ H2S-
>Black Red slant tip and gas: Gluc+ Lac- H2S+ (Salmonella Proteus) > Black yellow slantand gas: Gluc+ Lac+ H2S+
*NOTE: IF THERE IS GAS AT THE BOTTOM (AND ONLY THE VERY TIP TOP IS RED) THEN IT IS LAC+ |
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Term
WHAT IS THE ONLY POSITIVE IN THE UREASE TEST? |
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Definition
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Term
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Definition
Barrit's reagents.
VP1: Alpha-nahthol
VP2: KOH |
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Definition
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Term
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Definition
Set up for direct microscope count
usually performed in milk. Much quicker than a standard plate count |
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Term
Determining Actual Bacterial Count in DMC |
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Definition
1.Count the number of organisms seen in a single microscopic field of view
2.Count 9 additional fields of view
3.Add the number of organisms and divide the total by 10 to determine the average
4.Multiple the average by the Microscopic Factor previously determined
# Bacteria/ml = Average # bacteria X MF |
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Term
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Definition
Bacteria incubated in a small tube of plasma overnight.
If the plasma becomes clumpy or solidfies, the coagulase+
ONLY VALID ON GRAM+ STAPHYLOCCUS LIKE BACTERIA |
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Term
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Definition
- Staphylococcus-
- Aureus (Manitol/caogulase/DNase +)
- Epidermis (Manitol/caogulase/DNase -)
- Streptococcus
- Pneumoniae (Alpha hemolysis)
- Pyorgenes (Beta hemolysis)
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Term
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Definition
- Corynebacterium
- lactobacillus
- Sporosarcina
- Micrococcus
- Staphylococcus
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