Term
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Definition
| a standard maintenance medium(does not alter or influence growth or colony characteristics) |
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Term
| Phenylethanol Agar (PEA): |
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Definition
| selective medium that suppresses the growth of most g- while allowing the growth of most g+.(Aids in the isolation of g+ cocci from patients) |
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Term
| Urea broth Reading Results- |
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Definition
| Hot pink = +urease, dull pink = -test. |
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Term
| Citrate Agar Slant Reading Results- |
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Definition
| Any blue surface and/or butt = +for citrate utilization.(otherwise it will stay green) |
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Term
| PR lactose/sucrose/glucose/maltose Reading Results- |
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Definition
| Yellow = +fermentation, bubble = +gas |
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Term
| Nutrient Gelatin Reading Results |
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Definition
| Solidify= -test, liquid= +test gelatin hydrolysis (Place tube in ice bath rack for 20 minutes) |
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Term
| Tryptone broth Reading Results- |
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Definition
| (add a layer of Kovac’s reagent into tube)red+indole, yellow- |
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Term
| SIM agar Reading Results- |
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Definition
1.)Read tube for motility first, cloudiness away from stab = +motility
2.)(Add a layer of Kovac’s reagent) red+indole, yellow-
3.) If agar is blackened, test is + for H2S |
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Term
| Egg Yolk Agar Reading Results |
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Definition
| yellow agar = +lipid hydrolysis, red= -test (Place dropperful of phenol red on agar at colony edge. After 15 minutes read results.) |
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Term
| Starch Agar Reading Results- |
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Definition
| orange agar = +starch hydrolysis black/blue= -test (place dropperful of gram’s iodine on agar at colony edge. After 15 minutes read results) |
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Term
| MRVP Broth Reading Results |
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Definition
Tube 1: add ½ dropperful of alpha naphthol, and 40% potassium hydroxide
Cap tightly & shake vigorously, wait 10 minutes, shake again.
Red = +voges proskauer (AMC), all other colors are negative
Tube 2: add ½ dropperful of methyl red… red result is MR+, orange is –test |
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Term
| Eosin Methylene Blue (EMB) |
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Definition
-suppresses the growth of g+ while allowing the growth of g-
- G - that grow, this agar differentiates between those that ferment lactose and those that do not.Lactose fermenters=darker than the agar itself. (E.coli shows up metallic green.) |
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Term
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Definition
| differential and enriched medium that is a TSA base with 5% sheep’s blood to enhance the growth of pathogens. Differential medium based on the degree of hemolysis of RBCs in the agar. Be sure to make evaluations where colonies are sparse and isolated. Heavy thick growth displays ambiguous readings. |
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Term
| Blood Agar Reading Results |
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Definition
gamma hemolytic – no lysis of blood in agar
alpha hemolytic – partial or incomplete lysis of red blood cells in agar appears as a greenish, distinctly
bordered opaque zone around each colony
beta hemolytic – complete RBC lysis appears as clear, distinct opaque borders around each colony |
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Term
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Definition
| selective and differential medium used to isolate bacteria from stool cultures. This medium isolates most common intestinal pathogens. G+ bacteria are inhibited while Salmonella and Shigella species show up lightly colored or black-centered on this agar. Common non-pathogen coliforms (E. coli) show up brightly colored. |
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Term
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Definition
| selective and differential medium designed to isolate skin organisms and high salt tolerant organisms such as Staphylococcus. The selective factor is high salt concentration. The differential feature is the fermentation of mannitol sugar. The phenyl-red acid/base indicator turns yellow in low pH indicating fermenters while red or hot pink indicates neutral to high pH of non-fermenters. |
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Term
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Definition
| differential and enriched medium contains cooked blood and tellurium based salts. The salts differentiates those bacteria that can metabolize the tellurite. The blood enhances the growth of organisms such as Corynebacterium (for which the agar is designed). Black colonies occur with tellurite reducers. |
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Term
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Definition
| differential medium that selects for various species of Pseudomonas species. The agar accelerates the production of fluorescent pigments. This fluorescence is examined under UV light after incubation. |
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Term
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Definition
| agar displays fermentation of three sugars (triple) that this medium contains…glucose, sucrose, and lactose. Fermenters turn the phenyl-red acid/base indicator to yellow. Non-fermenters cause the medium to remain red. This medium also indicates amino acid metabolism by the production of hydrogen sulfide as a by-product. This is indicated by the formation of a brown-black precipitant when the hydrogen sulfide reacts with the iron in the medium. |
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Term
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Definition
| culture medium that allows the growth of certain types of organisms, while inhibiting the growth of other organisms |
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Term
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Definition
| culture medium that allows one to distinguish between or among different microorganisms based on a difference in colony appearance (color, shape, or growth pattern) on the medium. |
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