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Microbiology lab
Quiz 3
17
Biology
Undergraduate 4
10/07/2008

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Term
McFarland Standard
Definition
a way to determine cell density based on turbidity
Term
Antiboditcs
Definition
natural antimirobial agents that are produced by mircoorganisms
Term
Why Standardization?
Definition
aviod variation; evaluate one varible
Term
E-Test
Definition
an antimicrobial susceptibility test that can findthe minimun inhibitory concentration.
Term
lysozyme
Definition
an enzyme that degrades the peptidoglycan layer in cell walls causing cell lyses; 1st line of defense -- non-specific immune mechanism
Term
Enriched media
Definition
contains one or more additives to promote bacterial growth
Term
Selective media
Definition
contains on or more additives to suppress bacterial growth
Term
Differential media
Definition
contains an indicator to distinguish between different types of bacteria
Term
Mannitol Salt Agar Plate
Definition
selective -- micrococcus and staphylococcus (salt tolerance-growth)
differential -- mannitol fermentation (yellow)
Term
Phenylethanol Agar Plate
Definition
seletive -- Gram positive (phenyethanol-growth)
Term
Endo Agar
Definition
selective -- Gram-negative (growth)
differential -- lactose fermentation (gold sheen-fermenter/pink-weak fermenter)
Term
Eosin Methylene Blue Agar Plate
Definition
selective -- Gram-negative (growth)
differential -- lactose fermentation (gold sheen-fermenter/pink-weak fermenter)
Term
Hektoen Enteric Agar Plate
Definition
selective -- Gram-negative (growth)
differential -- lactose fermentation (yellow) and sulfur reduction (black colonies)
Term
Salmonella and Shigella Agar Plate
Definition
selective -- Gram-negative (growth)
differential -- lactose fermentation (pink) and sulfur reduction (black colonies)
Term
XLD Agar Plate
Definition
selective -- Gram-negative (growth)
differential -- xylose fermentation (yellow), lysine fermentation (pink), and sulfur reduction (black colonies)
Term
MacConkey Agar Plate
Definition
selective -- Gram-negative (growth)
differential -- lactose fermentation (pink)
Term
Blood Agar Plate
Definition
enriched -- 5% sheep RBC
differential -- hemolysis (beta-complete, alpha-partial, gamma-none)
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