Term
| What bacteria causes tuberculosis? |
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Definition
| Mycobacterium tuberculosis |
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Term
| What are some of the characteristics of Mycobacterium species? |
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Definition
Waxy lipid coating of mycolic acid. Protects against drying out Protects against attack by the IS
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Term
| What is a Mycobaterium species that is part of the normal flora? |
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Definition
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Term
| What Mycobacterium species causes leprosy? |
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Definition
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Term
| What is a unique characteristic of mycobateria that causes testing problems? |
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Definition
| It grows very slowly, 4-6 weeks to grow visible colonies. |
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Term
| Why is acid fact staining test a unique test? |
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Definition
It is confirmatory and only a strong indicator. Since colonies are needed for confirmation testing acid fast staining is used as a quick diagnostic tool. Acid fast results with symptoms are sufficient for diagnosis. It is also a differential staining method test. |
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Term
| What is the acid fact test procedue? |
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Definition
Collect sputum Prepare slide, heat fix Apply acid fast dyes (carbol fuchsin) View under OI lense Presence of acid fast bacilli confirm TB
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Term
| What is the positive control and why is it used? |
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Definition
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Term
| What is the negative control and what is it used for? |
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Definition
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Term
| What are the dying steps to acid fast staining? |
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Definition
Apply carbol fuchsin (primary pink dye) Decolorize - washes pink dye of non acid fast bacteria Methylene blue (counterstain blue dye) - stains bacteria that do not hold carbol fuchsin.
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Term
| What were the unknown sputum observations and results? |
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Definition
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Term
| What is the role of Mycobacterium smegmatis? |
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Definition
| A combination of exfoliated (shed) epithelial cells, transudated skin oils, and moisture, and can accumulate under the foreskin of males and within the vulva of females. It has a characteristic strong odor. Mycobacterium smegmatis is the characteristic bacterium involved in smegma production, and is generally thought to form smegma from epidermal secretions. |
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