Term
| What is the upper respiratory tract? |
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Definition
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Term
| What is the lower respiratory tract? |
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Definition
| Larynx, trachea, broncial tubes, and alveoli. |
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Term
What are some normal flora of the RT? |
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Definition
Staphylococcus (Most common, causes sore throat - acute pharyngitis), Steptococcus, Neisseria, and Haemophilis. These MO compete with pathogens and are considered antogonists.
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Term
| How can streptococci be identified? |
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Definition
Charastic antigens Hemolytic reactions in blood agar.
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Term
| What are the three types of hemolysis that can occur in blood agar? |
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Definition
Beta-hemolysis (complete) - clear zone around colony. Alpha-hemolysis (partial) - cloudy, greenish zone. Gamma-hemolysis - no hemolysis, unchanged.
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Term
| What are the results that can be concluded about streptococcus from hemolytic observations? |
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Definition
Alpha and Gamma streptococci are normal flora. Beta streptococcus are pathogens (S. pyogenes)
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Term
Blood agar is a differential and enrichment agar. Why? |
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Definition
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Term
| What is a quick test that can be used to differentiate between staphylococcus and streptococcus species? |
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Definition
| Catalase Test (Lab 14 Gram positive coccus flow chart) |
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Term
| What is the importance of microbial antagonism? |
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Definition
Normal flora surpress pathogens by competing for resources (nutrients, space) and preventing growth and infection. |
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Term
| What was the procedure for RT bacteria isolation and identification? |
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Definition
Swab throat, streak for isolation of blood agar plate (BAP). Incubate 18-24 hrs at 37 C Select isolated different colonies, observe characteristics and hemolytic reaction. Perform catalase test and alternative catalase test. Gram stain and record observations.
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