Term
| how do you differentiate between group A and group B strep? |
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Definition
| CAMP test (enhanced beta hemolysis at intersection of Beta hemolytic bacteria and group B strep) |
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Term
| how do you differentiate between group A and group B strep? |
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Definition
| CAMP test (enhanced beta hemolysis at intersection of Beta hemolytic bacteria and group B strep) |
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Term
| How do you differentiate between Group A strep and pneumococcus? |
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Definition
| BAP with optichin (p) and bacitracin (a )discs ... The optichin will kill the pneumococcus The Bacitracin will kill the strep. |
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Term
| how do you differentiate between H flu and H paraflu using a mueller hinton agar plate?? |
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Definition
| Using a mueller hinton agar place a disc with factor X (hemin) and factor V (NAD) -- the H flu will form satellites between the two discs the H paraflu will form satellites only around the factor V disc |
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Term
| How do you differentiate between H flu and H paraflu using chocolate agar? |
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Definition
| Only H flu will grow on chocolate agar. |
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Term
| what is the morphology of Neisseria menengitidis? How can we see it? |
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Definition
| Gram negative diplococcus, Gram stain |
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Term
| What is the morphology of H flu? How can we see it? |
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Definition
| Gram negative coccoacilus, gram stain |
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Term
| What is the morphology of the coxsackie virus, how can we see it? |
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Definition
| Its a virus! Negative stain with electron microscopy |
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Term
| What is the morphology of streptococcus pneumoniae? How can we see it? |
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Definition
| Gram positive coccus, gram stain. |
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Term
| what is the morphology of cryptococcus neoformans? How can we see it? |
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Definition
| Encapsulated yeast, Negative stain. |
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Term
| Describe a negative stain. Why do we use it? |
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Definition
| Preformed with nigrosin or india ink. Black background with white cells. Useful for observing larger cells, cell capsules and mucilage or for visualizing unsustainable cells |
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Term
| What does an oxidase test test for? what is a negative vs positive reaction? |
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Definition
| Tests for the production of cytochromec oxidase. positive reaction = dark blue, Negative reaction = light blue or colorless. |
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Term
| What does a catalase test test for? How do we differentiate a positive versus negative reaction? |
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Definition
| Tests for reproduction of catalase (degrades hydrogen peroxide) Positive = bubbles negative = no bubbles |
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Term
| What does a coagulase test test for? How can we differentiate a positive versus a negative reaction? |
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Definition
| Tests for the production of coagulase (which reacts with the clotting protein fibrin to allow a bacterium to become coated with fibrin and evade the immune system.) Positive= aggregated cells negative reaction= homogenous cell mixture |
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Term
| Between what vertebrae is a spinal tap preformed? |
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Definition
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Term
| What is the morphology of Staphylococcus aureus? |
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Definition
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Term
| What is the morphology of streptococcus sp? |
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Definition
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Term
| What is the morphology of proteus mirabilis? |
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Definition
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Term
| What is the morphology of enterococcus? |
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Definition
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Term
| What is the morphology of Pseudomonas aeruginosa? |
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Definition
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Term
| What is the morphology of Clostridium sp? |
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Definition
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Term
| What kind of agar is mannitol salt agar?What does it select for? What is its pH indicator? |
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Definition
| Selective and differential agar, selects for Staphylococcus and Micrococcus spp, differentiates mannitol fermenters (yellow) from non fermenters(red) Its pH indicator is phenol red. |
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Term
| What kind of agar is DNAse agar? What does it differentiate for? What is its pH indicator? |
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Definition
| Differential agar. Differentiates microbes that produce DNAse (magenta) from those that cant (blue). pH indicator= teledyne blue or toluidine blue. |
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Term
| Is E.coli Gram + or Gram -? Is it a normal intestinal flora or a pathogen? |
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Definition
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Term
| Is Enterobacter sp. gram + or gram -? is it a normal intestinal flora or is it a pathogen? |
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Definition
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Term
| Is Enterobacter faecalis Gram + or Gram -? Is it a normal intestinal flora or a pathogen? |
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Definition
| Gram +, it is both a normal flora and a pathogen. |
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Term
| Is Proteus sp gram + or gram -? Is it a normal intestinal flora or is it a pathogen? |
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Definition
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Term
| Is Pseudomonas sp Gram + or gram -?, is it normal intestinal flora or is it a pathogen? |
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Definition
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Term
| Is salmonella sp gram + or gram -? Is it a normal intestinal flora or is it a pathogen? |
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Definition
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Term
| Is shigella sp gram + or gram-? Is it a normal intestinal flora or is it a pathogen? |
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Definition
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Term
| What does MacConkey agar select and differentiate for and against? How? What is its indicator dye? |
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Definition
| Selective for Gram negative bacteria, Differentiates lactose fermenters (pink) from non fermenters (colorless). Indicator dye = neutral red |
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Term
| What does XLD agar select for? What does it differentiate between and how? What is its pH indicator? |
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Definition
| XLD selects for Gram - bacteria, It differentiates Xylose and Lysine fermenters (red) and H2S gas producers (black centers)Salmonella is red with a black center and shigella is red. pH indicator = phenol red |
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Term
| What does Bile Esculin Agar select for? What does it differentiate between? |
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Definition
| Selects for fecal streptococci and Enterococcus (but against other Gram + bacteria and strep). Differentiates between Enterococcus (black ppt) and Streptococcus (gold) |
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Term
| How does proteus behave on Blood agar? |
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Definition
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Term
| What does Urease agar differentiate between? what is its pH indicator? |
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Definition
| Urease differentiates between urea metabolizers (pink). Its pH indicator is phenol red. |
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