Term
What is the shape of Ecoli |
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Definition
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Term
Microscope that shows dark objects in a brighter field |
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Definition
A brightfield compound microscope |
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Term
What are the basic parts of a microscope? |
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Definition
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base
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stage
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arm
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body tube
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condenser
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iris diaphragm
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objective lenses
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ocular lens
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coarse adjustment knob
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fine adjustment knob
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Term
What are the 4 possible objective lense on a normal compound microscope and what are their powers? |
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Definition
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4 x - scanning power
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10 x - low power
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40 x - high power
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100 x - immersion oil
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Term
Refers to the ability of lenses to reveal fine detail or 2 points distinctly separated |
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Definition
resolution or resolving power |
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Term
A magnifying lense with 10 x power and part of the eyepiece |
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Definition
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Term
The total magnification obtained when the 4x (scanning) lens is in place is? |
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Definition
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Term
The total magnification obtained when the 10 x (low power) lens is in place ? |
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Definition
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Term
A culture that only contains one kind of microorganism |
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Definition
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Term
A differential stain to be use to differentiate between microorganisms |
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Definition
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Term
What are the 4 Gram staining regents used? |
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Definition
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Crystal violet
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Iodine
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Decolorizing agent
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safran
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Term
What color appears on a gram negative organism ? |
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Definition
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Term
What color does a gram positive organism appears
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Definition
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Term
Why is agar preferable to gelatin as a solidifying agent in culture media? |
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Definition
agar has no nutritional value so bacteria cannot feed off of it. The agar remains solid until it is melted at 100 degrees celcius and then cooled at 40 degrees celcius where it remains solid. Solid agar is better for bacteria to grow because microbes can't degrade it. |
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Term
What can enhance resolving power? |
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Definition
1) To shorten the distance from the objective lens and the ocular lense. Smaller wavelengths of light improve resolving power. |
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Term
The main functions of a microscope |
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Definition
Resolution and Magnification of a compound microscope |
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Term
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Definition
They refer to the ability of a microscope to distinguish between two objects. |
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