Term
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Definition
| all living cells are destroyed or removed |
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Term
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Definition
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Term
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Definition
| treatment of an object or surface to make it safe to handle |
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Term
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Definition
| killing, inhibition, or removal of pathogens |
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Term
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Definition
| normally for inanimate objects |
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Term
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Definition
| used on tissues to prevent infection |
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Term
| Factors that affect death rate |
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Definition
Microbial load
Population Composition
Length of Exposure
Concentration of antimicrobial agent
Local environment |
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Term
| Two examples of population composition |
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Definition
Vegetative cells vs endospores
Young cells vs old cells |
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Term
| What is the most commonly used method of sterilization? |
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Definition
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Term
| Temperatures above maximum lead to |
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Definition
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Term
| What kind of heat has more penetrating power? |
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Definition
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Term
| What are two types of heat sterilization |
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Definition
| autoclaving, pasteurization |
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Term
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Definition
| time required to reduce the population by one log order at a given temperature |
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Term
| Relationship between D and temperature is |
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Definition
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Term
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Definition
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Term
| Autoclave is basically steam |
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Definition
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Term
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Definition
| increases the temperature at which water boils |
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Term
| Water under two atmospheric pressures boils at what temperature? |
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Definition
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Term
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Definition
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Term
| Do beverages get sterilized? |
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Definition
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Term
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Definition
Heat liquid while retaining flavor and food value
Reduces growth of spoilage organisms
Prevent transmission of milk-borne pathogens |
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Term
| Two types of pasteurization |
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Definition
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Term
|
Definition
| Exposure at 71.6 degrees for 15 seconds (flash) |
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Term
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Definition
| 63-66 degrees celcius for 30 minutes (vat) |
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Term
| Does pasteurization kill endospores? |
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Definition
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Term
|
Definition
| incinerator (Bunsen Burner) |
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Term
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Definition
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Term
|
Definition
| heat-resistant metals and glass |
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Term
| Hospitals used incineration |
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Definition
| to destroy infectious materials |
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Term
| What dry heat can kill endospores? |
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Definition
| Dry oven (150-180°C) for 2-4 hours |
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Term
| Lethality of dry oven is due to |
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Definition
| oxidation of key molecules and/or dehydration of the cell |
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Term
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Definition
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Term
|
Definition
| thymine-thymine dimers which are mutagenic |
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Term
| UV light does not penetrate |
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Definition
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Term
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Definition
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Term
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Definition
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Term
| Reacts with adjacent molecules, causing |
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Definition
| aberrations and breakage of DNA |
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Term
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Definition
| amount needed to reduce the number by a log value |
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Term
| Decimal reduction is given in |
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Definition
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Term
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Definition
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Term
| Killing (lethal) dose for radiation sterilization is |
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Definition
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Term
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Definition
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Term
| Filtration is effective at sterilizing |
|
Definition
| liquids that are heat sensitive |
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Term
| Different materials used for filtration |
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Definition
| Glass fibers, paper, cellulose acetate |
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Term
|
Definition
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Term
|
Definition
| depth, membrane, nucleopore |
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Term
| Two types of depth filters |
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Definition
Paper , Borosilicate (bigger substances get trapped)
HEPA (high efficiency particulate air) filter |
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Term
| Two types of membrane filters |
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Definition
Cellulose acetate, Cellulose
nitrate |
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Term
|
Definition
Polycarbonate film treated
with nuclear radiation |
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Term
| Nucleopore filters are used |
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Definition
| Isolate specimens for scanning electron microscopy |
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Term
| Chemical agents need rapid action at |
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Definition
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Term
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Definition
| Broad-spectrum without being toxic to animals |
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