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Microbiology Exam #2 Review
Metabolism, Nutrition, and Genetics
79
Microbiology
Undergraduate 1
02/21/2015

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Term
cytoplasm
Definition
Glycolysis takes place in the _______
Term
Glycolysis
Definition
Glucose is broken into two pyruvates through an oxidation-reduction reaction during which glucose is oxidized and NAD is reduced to form NADH.
Term
2 pyruvates
2 ATP
2 NADH
Definition
Glycolysis produces:
Term
Phosphorylation
Definition
ATP is formed through ________ of ADP.
Term
Fermentation
Definition
The conversion of pyruvate to alcohols and acids
Term
Pasteurization
Definition
Heating food to between 55-60C to get rid of bacteria and then cover it up.
Term
Matrix of the mitochondria
Definition
The Krebs cycle occurs in the _________.
Term
Acetate
Definition
Two carbons formed from the decarboxylation of the three carbon pyruvate in the Krebs cycle.
Term
Acetyl CoA
Definition
Transfers electrons to organize acids in the Krebs cycle
Term
2 ATP
6 NADH
2 FADH
2 CO2
Definition
The Krebs cycle produces:
Term
cristae
Definition
The electron transport chain takes place in the _________.
Term
Electron transport chain
Definition
2 FADH and 8 NADH attach to the cristae in order to detach energy from their electrons
Term
32 ATP
Definition
The electron transport chain produces:
Term
36 ATP
Definition
one molecule of glucose produces:
Term
oxygen
Definition
_______ is the final receptor of electrons
Term
culture
Definition
Growth of organism outside the body is called _______.
Term
Lag phase
Definition
intense activity preparing for population growth, but no increase in population.
Term
Log phase
Definition
Logarithmic or exponential increase in population.
Term
Stationary phase
Definition
Period of equilibrium where microbial deaths balance the production of new cells.
Term
Death phase
Definition
Population is decreasing at a logarithmic rate.
Term
J-curve
Definition
Lag phase and Log phase is called the ______.
Term
S-curve
Definition
Stationary and death phase is called the ______.
Term
Microbial growth
Definition
Growth in number not size microbes.
Term
Broth
Definition
liquid media
Term
Discrete colony
Definition
result of microbial growth; an aggregation of cells arising from a single parent cell
Term
Chemically defined media
Definition
Growth of chemoautotrophs and photoautotrophs; microbiological assays (used in research).
Term
Complex media
Definition
Growth of most chemo-heterotrophic organisms; can be formed by food.
Term
Reducing media
Definition
Growth of obligate anaerobes; (sodium thioglycolate)
Term
Selective media
Definition
Suppression of unwanted microbes; encouraging desired microbes.
Term
Differential media
Definition
Differentiation of colonies of desired microbes from others.
Term
Enrichment media
Definition
Similar to selective media but designed to increase the number of desired microbes to detectable levels.
Term
Autotrophs
Definition
organisms that use inorganic carbon sources to create energy containing organic molecules; plants are a prime example.
Term
Heterotrophs
Definition
Organisms that catabolize food sources from organic molecules; animals
Term
Photoautotrophs
Definition
Plants, algae, and cyanobacteria that use H20 to reduce CO2, producing O2 as a byproduct.
Term
Photoheterotrophs
Definition
Green non-sulfur and purple non-sulfur bacteria; rare.
Term
Chemoautotrophs
Definition
Hydrogen, sulfur, and nitrifying bacteria.
Term
most animals, fungi, protozoa, and many bacteria
Definition
What organisms undergo aerobic respiration?
Term
some animals and bacteria
Definition
What organisms undergo anaerobic respiration?
Term
Chemoheterotrophs
Definition
The most common organisms that undergo aerobic or anaerobic respiration
Term
Facultative anaerobes
Definition
can maintain life via fermentation or anaerobic respiration or by aerobic respiration; (commonly infect humans).
Term
Aerotolerant anaerobes
Definition
Does not use aerobic metabolism but contains some enzymes that detoxify oxygen's poisonous forms
Term
Microaerophiles
Definition
microbes that require oxygen within levels of 2-10%
Term
Nitrogen fixation
Definition
The reduction of nitrogen gas to ammonia; essential to life on earth.
Term
760mmHg
Definition
Atmospheric pressure
Term
Psychrophiles
Definition
organisms that grow optimally at around 10C
Term
Mesophiles
Definition
Microorganisms that grow optimally at 35-40C; can grow in human bodies.
Term
Thermophiles
Definition
microorganisms that grow optimally at 65-70C
Term
Hyperthermophiles
Definition
microorganisms that grow optimally at 95-100C
Term
DNA
Definition
A-T-C-G
Term
RNA
Definition
A-U-C-G
Term
36
Definition
Respiration produces _______ ATP from one molecule of glucose.
Term
water and CO2
Definition
The two waste products of respiration are ________ and _______.
Term
Genome
Definition
the entire genetic complement of an organism; nucleotides and genes.
Term
Prokaryotic genome
Definition
DNA not in nucleus, nucleoid that is found in cytoplasm, 1 DNA, circular DNA, and contained in two structures; chromosome and plasmid.
Term
Eukaryotic genome
Definition
DNA in nucleus, 2 or more DNA, linear DNA.
Term
Homologues and autosomes (similar)
Definition
Chromosomes 1-22 in a human karyotype are ________
Term
non-homologous or heterologous
Definition
Chromosome 23 in a male human karyotype is ________
Term
Chromosome 23 in human male
Definition
XY
Term
Chromosome 23 in human females
Definition
XX
Term
semi-conservative
Definition
DNA replication is __________
Term
Interphase
Definition
DNA replication occurs during the ________ of mitosis
Term
Prophase
Definition
The cell loses the nuclear membrane during the _________ in mitosis.
Term
Metaphase
Definition
Chromosomes align in the middle during the ________ of mitosis
Term
Anaphase
Definition
The distribution of chromosomes in the upper and lower portion of the cell occurs during the ______ in mitosis.
Term
Telophase
Definition
Two new nuclear membranes are formed and cytokinesis begins to take place by invagination of cell membrane; this occurs in the _______ of mitosis.
Term
Barophile
Definition
bacteria that lives in certain pressures such as hydrostatic pressure
Term
colony forming unit
Definition
CFU
Term
Lungs
Definition
Sputum comes from the _______.
Term
Spermatogonia
Definition
Immature male sex cells
Term
Oogonia
Definition
immature female sex cell
Term
Spermatozoa
Definition
mature haploid male sex cell with tails
Term
Egg
Definition
Mature haploid female sex cell.
Term
Independent assortment
Definition
DNA is exchanged with random homologous chromosomes; you never know where each chromosome is going to end up.
Term
Transfer RNA (tRNA)
Definition
picks up all of the amino acids needed to form the proteins and goes into the rough ER.
Term
Ribosomal RNA (rRNA)
Definition
Prepares the ribosome which is where the cell prepares proteins.
Term
Messenger RNA (mRNA)
Definition
Initiates protein synthesis.
Term
mRNA, tRNA, and rRNA
Definition
Transcription produces ______
Term
proteins
Definition
Translation produces ______.
Term
Embryo
Definition
Formed when a spermatozoa enters the egg and fertilizes creating a diploid cell.
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