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study of the diversity of organisms and their relationships |
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What does systematics link? |
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the science of biological classification |
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determining that a particular isolate belongs to a recognized taxon |
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assignment of names to the taxonomic group |
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What did taxonomy used to rely on? |
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Now what approach is used in taxonomy? |
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What are the three parts of polyphasic |
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Phenotypic Genotypic Phylogenetic |
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What are the five parts of phenotypic analysis? |
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Motility, Morphology, Metabolism, Physiology, and Cell Chemistry |
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colony morphology, Gram reaction, cell size and shape |
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Nonmotile, gliding, swimming, swarming motility |
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Utilization of C, N2 or S compounds, fermentation of sugars, N2 fixation |
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Temperature, pH and salt ranges for growth, response to O2, production of extracellular enzymes |
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Fatty acids, polar lipids |
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FAME fatty acid methyl ester |
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variation in type and proportion of fatty acids present in membrane lipids |
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Why does FAME require rigid standardization? |
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because FAME profiles can vary as a function of temperature, growth phase, and growth medium |
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What does genotypic analysis provide? |
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Information at the DNA level |
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What are the four types of genotypic analysis? |
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DNA-DNA hybridization DNA profiling methods Multilocus Profiling GC ratios |
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focuses on identifying species within a genus |
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Focuses on identifying strains within a species |
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Focuses on multiple genes |
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Percentage of guanine plus cytosine bp in the genome |
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complation of both the phenotypic and genotypic info Based on analysis of Individual genes – 16S rRNA Multiple genes Whole genome |
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What percent is the same species when comparing DNA-DNA hybridization |
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What percent is the same species in 16s RNA gene sequence identity |
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The International Code of Nomenclature of Bacteria |
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Taxonomic outline of the prokaryotes |
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Second edition of Bergey’s Manual of Systematic Bacteriology |
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compendium of information on all recognized species of bacteria |
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provides detailed information on the enrichment, isolation, and culture of the many groups of Bacteria and Archaea |
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