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Microbiology Exam 1
N/A
203
Biology
Undergraduate 4
09/22/2014

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Cards

Term
All electron donors use the same dehydrogenase complex.
Definition
False
Term
A typical prokaryotic nucleoid contains multiple linear chromosomes.
Definition
False
Term
Darkfield microscopy is optimal for visualizing unstained microbial cells that are particularly small
Definition
True
Term
Pure culture technique requires microbe isolation, microbe transfer and pasteurization
Definition
False
Term
Koch's postulates define the familial inheritance patterns sufficient to prove the genetic origin of a disease.
Definition
False
Term
Complex media enhance or discourage the growth of particular microbes
Definition
False
Term
Gram-negative cells have an outer membrane external to their peptidoglycan layer
Definition
True
Term
Protein molecule function can be changed by denaturation
Definition
True
Term
Reverse transcription is an exception to the Central Dogma of Biology
Definition
True
Term
Chlorophyll uses reversible oxidation and reduction of a centralized iron atom to carry electrons
Definition
False
Term
Plate counts can be used to enumerate dead microbial cells within a culture
Definition
False
Term
Fermentation produces end products at a much lower average redox level than the substrates
Definition
False
Term
Some prokaryotic cells secrete a protein s-layer to establish biofilms
Definition
False
Term
Antibiotic resistance is not a concern when treating tuberculosis
Definition
False
Term
Microbes represent around 20% of the Earth's biomass
Definition
False
Term
In a batch culture, primary microbial metabolites are generated during the balanced/exponential growth phase
Definition
True
Term
The most common method for liquid sterilization is filtration.
Definition
False
Term
The total magnification power of a compound microscope is the product of the ocular and objective lens magnifications.
Definition
True
Term
Approximately 70% of a typical prokaryotic cell is water
Definition
True
Term
Photoautotrophs oxidize chemical compounds for energy.
Definition
False
Term
Type I and Type II reaction centers accept electrons from cytochrome C or plastocyanin to return chlorophyll to its ground state.
Definition
True
Term
A photosynthetic reaction center is efficient at absorbing light in the absence of pigment antenna
Definition
False
Term
The reverse TCA cycle is used by autotrophic bacteria for nitrogen fixation
Definition
False
Term
Most prokaryotic cells divide using binary fission
Definition
True
Term
Proteins macromolecules are polymers of amino acids linked by peptide bonds
Definition
True
Term
Replication of the bacterial chromosome begins at the ori site.
Definition
True
Term
Cyclic photophosphorylation is only used during autotrophic growth
Definition
False
Term
Antoni van Leeuwenhoek's simple microscope offered high quality images with less distortion
Definition
True
Term
Chemoautotrophy allows some microbes to occupy unique ecological niches
Definition
True
Term
Protein molecules at the tip of pili are termed adhesins
Definition
True
Term
A typical prokaryotic cell has more than ten thousand ribosomes present in the cytoplasm
Definition
True
Term
All electron transport chains have a dehydrogenase protein complex
Definition
True
Term
Biological safety cabinets use pressurized steam to sterilize incoming air and prevent contaminated air from escaping
Definition
False
Term
Transmission electron microscopy (TEM) permits 3-D visualization of specimens without the use of computer software
Definition
False
Term
Prokaryotic cells in ocean surface water use proteorhodopsins to make energy from colored light
Definition
True
Term
Ionic potential is generated from reduced nucleotides such as NADH
Definition
True
Term
Expression of the nitrogenase operon is regulated so nitrogen fixation is only initiated when other nitrogen sources are available.
Definition
False
Term
Most prokaryotic environments have higher solute concentrations than the cytoplasms of prokaryotes that live in them
Definition
False
Term
Shadow-casting SEM generates 3D images of internal cell structures
Definition
False
Term
Prokaryotic organisms come in a variety of different shapes and arrangements
Definition
True
Term
The smallest unit of life is a cell.
Definition
True
Term
The growth temperatures of thermophiles span a range between 40°C and 80°C.
Definition
True
Term
Escherichia coli (E. coli) is a model system for understanding prokaryotic growth, gene regulation and core metabolism.
Definition
True
Term
Photophosphorylation and respiration generate energy in the absence of ATP synthase
Definition
False
Term
The Mycobacterium envelope has a thick capsule that contains polysaccharides, proteins and mycolic acid
Definition
True
Term
Prokaryotic inclusions are used for motility or the storage of metabolites
Definition
True
Term
The most common archaeal envelope resembles the Gram-negative envelope
Definition
False
Term
Facultative anaerobes cannot grow in the presence of oxygen
Definition
False
Term
The transpeptidation reaction in peptidoglycan synthesis is blocked by beta-lactam antibiotics
Definition
True
Term
Fluorophores are illuminated by an emitter wavelength of light from a microscope lamp.
Definition
False
Term
Twitching motility requires the attachment and retraction of type 3 pili.
Definition
False
Term
Almost all prokaryotic cells have a nuclear membrane around their nucleoid
Definition
False
Term
An intact cell membrane is necessary for energy generation via respiration
Definition
True
Term
Retinal-based photophosphorlyation requires only a single intermembrane protein complex to pump protons
Definition
True
Term
Anaerobic chambers are required for cultivating microaerophilic microbes
Definition
False
Term
Batch culture growth passes through two distinct phases - growth phase and death phase
Definition
False
Term
The total magnification of a compound microscope is twice the magnification of the objective lens.
Definition
False
Term
Fermentative microbes make ATP via substrate-level phosphorylation
Definition
True
Term
RNA molecules are created from DNA template sequences via the process of translation
Definition
False
Term
The most common CO2 fixation pathway is the Ljungdahl-Wood pathway
Definition
False
Term
All archaeal envelopes contain a rigid pseudopeptidoglycan wall
Definition
False
Term
Phase-contrast and darkfield microscopy both allow visualization of living organisms.
Definition
True
Term
DNA gyrase is involved in prokaryotic genome supercoiling and replication
Definition
True
Term
The nitrogenase enzyme requires 16 moles of ATP to fix one mole of dinitrogen
Definition
True
Term
Cardinal growth temperatures for each microbial species or strain can vary dependent on growth conditions
Definition
False
Term
A primary function of the prokaryotic cell wall is to prevent uncontrolled cytoplasmic expansion
Definition
True
Term
Arrangements of prokaryotic cocci can include individual cells, chains and clusters
Definition
True
Term
Methanogenesis is a form of anaerobic respiration found in both eukaryotic and prokaryotic organisms.
Definition
False
Term
Transpeptidation connects peptidoglycan chains to create a sacculus structure in gram-negative bacteria.
Definition
True
Term
Microbial fermentations generate energy in the form of ATP
Definition
True
Term
Phycobilins are light-harvesting complexes located within chlorosomes
Definition
False
Term
Due to spontaneous mutations, a pure culture can develop many subpopulations that vary from the progenitor cells in nucleotide sequence
Definition
True
Term
Bioreactors in chemostat mode maintain constant nutrient concentrations
Definition
True
Term
The coupling of transcription and translation contributes to gene regulation in prokaryotes
Definition
True
Term
Protein synthesis is performed in polymerase complexes.
Definition
False
Term
Tuberculosis is caused by Mycobacterium tuberculosis
Definition
True
Term
Fluorescent microscopy relies on linking molecules to compounds called fluorophores.
Definition
True
Term
Transmission electron microscopy (TEM) requires substantial fixation and staining of specimens
Definition
True
Term
Prokaryotic inclusions are used exclusively for nutrient storage
Definition
False
Term
Gram-positive cells have an outer membrane to protect their peptidoglycan layer
Definition
False
Term
Chemoautotrophs utilize organic carbon compounds for biosynthesis.
Definition
False
Term
Bacillus subtilis endosporulation is a good model system for understanding developmental biology.
Definition
True
Term
All microbial growth media are considered either selective or differential media
Definition
False
Term
Freeze-etch EM allows better 3-D resolution of specimen surface features
Definition
False
Term
Biological safety cabinets use HEPA filters to prevent contaminated air from escaping
Definition
True
Term
Plants and animals arose on Earth over three billion years ago
Definition
False
Term
Chlorophyll uses reversible oxidation and reduction of a centralized magnesium ion to carry electrons
Definition
True
Term
Polysaccharides are polymers of sugars linked by phosphodiester bonds
Definition
False
Term
Type I photosystems use ferredoxin as an electron carrier
Definition
True
Term
Autoclaving sterilizes materials using pressurized steam.
Definition
True
Term
Joseph Lister developed post-surgical disinfection methods to prevent microbial infections
Definition
True
Term
The reduction of nitrate to nitrogen gas (N2) is a form of anaerobic respiration
Definition
True
Term
Most prokaryotic cells divide using multiple fission.
Definition
FALSE
Term
Bioreactors in chemostat mode are optimal for harvesting microbial cells or cell products.
Definition
False
Term
Prokaryotic life appeared on Earth over two billion years before plant and animal life.
Definition
True
Term
DNA gyrase is involved in prokaryotic genome supercoiling but not genome replication.
Definition
False
Term
In nitrogen fixation, ferredoxin reduces the nitrogenase two subunit
Definition
False
Term
Gliding motility can involve a slime extrusion mechanism or caterpillar mechanism for movement.
Definition
True
Term
All known electron transport chains require oxygen as a terminal electron acceptor.
Definition
False
Term
Bunsen burners are used for sterilization in microbial transfer techniques.
Definition
True
Term
Obligate anaerobes are killed in the presence of small amounts of oxygen.
Definition
True
Term
Transmission electron microscopy (TEM) requires substantial fixation and staining of specimens.
Definition
True
Term
Magnetosomes help bacteria distinguish north from south.
Definition
True
Term
The Planctomyces envelope has a protein layer located between two phospholipid membranes
Definition
True
Term
The most common archaeal envelope is the S-layer envelope
Definition
True
Term
The use of different electron donors requires the production of different dehydrogenase complexes
Definition
True
Term
Methanogenesis is a form of anaerobic respiration exclusive to archaea.
Definition
True
Term
The Gram-positive envelope is comprised of a single phospholipid cell membrane with multiple external peptidoglycan layers.
Definition
True
Term
A pure culture is a clonal population of genetically identical cells
Definition
False
Term
Microbial fermentations generate energy in the form of ionic potential
Definition
False
Term
Arrangements of spiral prokaryotes include individual cells, chains or clusters
Definition
False
Term
Type I reaction centers accept electrons from cytochrome C, while Type II reaction centers accept electrons from ferredoxin.
Definition
False
Term
Cyanobacterial photophosphorylation uses plastocyanin to return chlorophyll to a ground state
Definition
True
Term
The ability to distinguish between two tiny, close objects is called resolution.
Definition
True
Term
Tuberculosis is caused by Streptobacterium tuberculosis
Definition
False
Term
Peptidoglycan has cross-links formed by peptides
Definition
True
Term
Microbial growth media are considered either complex or chemically defined media
Definition
True
Term
Antoni van Leeuwenhoek discovered microbes in the 1700s after creating a high quality glass lens
Definition
True
Term
Proteorhodopsins perform photophosphorylaton using chlorophyll instead of retinal.
Definition
False
Term
Saccharomyces cerevisiae endosporulation is a good model system for studying prokaryotic developmental biology.
Definition
False
Term
The most common CO2 fixation pathway is the Calvin Benson cycle.
Definition
True
Term
The internal osmotic pressure of most prokaryotic cells is much lower than the osmotic pressure of the surrounding environment
Definition
False
Term
DNA and RNA macromolecules are polymers of nucleoside bases linked by glycosidic bonds
Definition
False
Term
Noncyclic photophosphorylation requires a steady supply of electron donors
Definition
True
Term
Prokaryotic osmoregulation involves maintaining a higher solute concentration in the cytosol than the external environment
Definition
True
Term
Biosafety levels range from BSL4 for nonpathogenic microbes up to BSL1 for serious health threats
Definition
False
Term
All microbes are prokaryotic
Definition
False
Term
Fluorophores are illuminated by an exciter wavelength of light from a microscope lamp
Definition
True
Term
A typical prokaryotic cell has only a few ribosomes present in the cytoplasm
Definition
False
Term
Most prokaryotic environments are more dilute than the cytoplasms of prokaryotes that live in them
Definition
True
Term
Ionic potential is used in most energy-requiring biosynthesis reactions
Definition
False
Term
Proteins macromolecules are polymers of amino acids linked by peptide bonds
Definition
True
Term
Chlorophyll-based photosynthesis cycles through excitation, oxidation, and reduction states
Definition
True
Term
The reduction of nitrate to nitrogen gas (N2) is called denitrification
Definition
True
Term
The stability of the dinitrogen bond makes nitrogen fixation an energetically expensive process
Definition
True
Term
Immersion oil is used on light microscopes to increase the refractive index between the lens and a specimen.
Definition
True
Term
Some prokaryotic cells can secrete capsular polysaccharides to establish biofilms
Definition
True
Term
Darkfield microscopy is optimal for visualizing unstained microbial cells that are particularly small
Definition
True
Term
Scanning electron microscopy (SEM) requires fixation and dehydration prior to coating
Definition
True
Term
Transmission electron microscopy removes the need for fixation and staining of specimens to provide better, more accurate images
Definition
False
Term
Respiration requires both an initial electron donor and final electron acceptor
Definition
True
Term
Chemoheterotrophs fix carbon dioxide for use in biosynthesis
Definition
FALSE (chemoautotrophs)
Term
Proteins represent about 62% of the macromolecular weight of an average E. coli cell
Definition
True
Term
All archaeal envelopes contain a rigid pseudopeptidoglycan wall
Definition
FALSE (s-layers do not)
Term
In chemoautotrophs, oxidation of electron donors often occurs in the cytoplasm
Definition
FALSE (perriplasm)
Term
The Planctomyces envelope has a protein layer external to its cell envelope
Definition
FALSE (in the middle)
Term
The reverse TCA cycle is used by autotrophic bacteria for nitrogen fixation
Definition
FALSE (carbon)
Term
Prokaryotic cells lack mitochondria and/or chloroplasts
Definition
True
Term
Type II noncyclic photophosphorylation requires reverse electron transport to fix carbon dioxide
Definition
True
Term
Robert Koch developed enrichment media for the growth and isolation of infectious disease agents
Definition
True
Term
Polyamines neutralize the charge of DNA allowing compaction of the DNA within the nucleoid
Definition
True
Term
Peptidoglycan has cross-links formed by peptides
Definition
True
Term
Gas vesicles provide nutrients to aquatic prokaryotic cells
Definition
FALSE - Acidocalcisomes
Term
Septation during binary fission is dependent on a ring of Min proteins at the equator of the cell after DNA replication
Definition
False
Term
Chemiosomotic potential can be converted to ATP by an enzyme called ATP interconvertase
Definition
False
Term
Generation time can be measured using spectrophotometry
Definition
True
Term
Type II reaction centers use quinone reductases to reduce quinone to quinol
Definition
True
Term
Antoni van Leeuwenhoek discovered microbes in the early 1700s after creating a high quality compound microscope
Definition
False
Term
Many chemoheterotrophs use fermentation to generate ATP when oxygen is absent
Definition
True
Term
The Central Dogma of Biology describes the transfer of genetic information as: RNA to DNA to protein
Definition
False
Term
A typical prokaryotic nucleoid contains a single, circular chromosome
Definition
True
Term
All archaeal envelopes contain a rigid pseudopeptidoglycan wall
Definition
False
Term
Type I photosystems use quinone as an electron carrier
Definition
FALSE- (Type 1 uses ferrodoxin as a electron acceptor and cytochrome-C gives 2 electrons )
Term
Flagella rotate by the entry of ions through the basal body
Definition
True
Term
Chemically defined media are composed of exact amounts of purified compounds
Definition
True
Term
Anaerobic chambers are required for cultivating microaerophilic microbes
Definition
False
Term
Haloarchaea photosynthesize using carotenoids instead of bacteriorhodopsins
Definition
False
Term
Robert Koch developed post-surgical disinfection methods to prevent microbial infections
Definition
False
Term
Macromolecules comprise 87% of the dry weight of an average E. coli cell
Definition
True
Term
Protein synthesis is performed in ribosome complexes.
Definition
True
Term
DNA and RNA are polymers of nucleotides connected by phosphodiester bonds
Definition
True
Term
The coupling of transcription and translation slows the genetic response to environmental change
Definition
False more rapid
Term
In nitrogen fixation, ferredoxin reduces the nitrogenase one subunit
Definition
True
Term
Interdivision time can be measured using spectrophotometry
Definition
FALSE (interdivision should be generation)
Term
A pure culture of microbes is descended from one or more similar cells
Definition
True
Term
Capsules form outside the cell envelope of some bacteria
Definition
True
Term
Cardinal growth temperatures are intrinsic to each microbial species or strain
Definition
True
Term
The similarity of central metabolism pathways across organisms is evidence against universal descent from a common ancestor
Definition
False (for)
Term
Peptidoglycan has cross-links formed by glycan molecules
Definition
False
Term
Teichoic acids are present in the peptidoglycan of Gram-positive organisms
Definition
True
Term
In chemoautotrophs, oxidation of electron donors often occurs in the periplasm
Definition
True
Term
The periplasm of a prokaryotic cell is a dense suspension of ribosomes.
Definition
False
Term
Sterilization of media by an autoclave led to the development of Tyndalization
Definition
False
Term
Oxidase-negative organisms lack both a cytochrome oxidase and a quinol oxidase
Definition
False
Term
Brightfield microscopy is optimal for visualizing unstained microbial cells that are particularly small
Definition
False (stained)
Term
Bioreactors in batch culture mode mimic the growth of most organisms in nature
Definition
False
Term
Green sulfur bacteria have pigment antenna called phycobilins
Definition
False (chlorosomes)
Term
Gas vesicles provide buoyancy to aquatic prokaryotic cells
Definition
True
Term
Louis Pasteur’s swan-neck flask experiments helped prove Spontaneous Generation
Definition
False (Germ Theory)
Term
Archaeal flagella are identical in structure to bacterial flagella
Definition
False
Term
Salmonellosis can be controlled by the proper storage, handling and cooking of raw fish
Definition
False (chicken)
Term
Mesophiles are organisms that prefer growth under conditions of high extracellular solute concentrations
Definition
False
Term
Bacterial cytokinesis is initiated by the MreB protein
Definition
False
Term
Cyclic Type I/II photophosphorylation uses water as an electron donor
Definition
False (non cyclic)
Term
The most common method for liquid sterilization is autoclaving
Definition
True
Term
A typical prokaryotic nucleoid contains a single, circular chromosome
Definition
True
Term
Two of the three domains of cellular life on Earth are exclusively microbial
Definition
True
Term
Prokaryotic organisms contain sterols in their cell membranes
Definition
False
Term
Biosafety levels range from BSL1 for nonpathogenic microbes to BSL4 for serious health threats
Definition
True
Term
An intact cell membrane is not used for energy generation by respiration
Definition
False
Term
The small molecules of a prokaryotic cell are in constant flux
Definition
True
Term
MreB is a prokaryotic cytoskeleton protein expressed in rod-shaped microbes
Definition
True
Term
Since chemoautotrophs use electrons for carbon fixation, they produce less ATP than chemoheterotrophs
Definition
True
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